Characterization and Comparison of Steroidal Glycosides from <i>Polygonatum</i> Species by High-Performance Liquid Chromatography–Electrospray Ionization Mass Spectrometry
<i>Polygonatum</i> species have been used as traditional medicines and functional foods in Asia and Europe since ancient times. In this study, a fast and simple method based on liquid chromatography coupled with electrospray ionization mass spectrometry (LC-ESI-MS) was developed to syste...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/2/705 |
| _version_ | 1797438318319763456 |
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| author | Danyang Liu Takashi Kikuchi Wei Li |
| author_facet | Danyang Liu Takashi Kikuchi Wei Li |
| author_sort | Danyang Liu |
| collection | DOAJ |
| description | <i>Polygonatum</i> species have been used as traditional medicines and functional foods in Asia and Europe since ancient times. In this study, a fast and simple method based on liquid chromatography coupled with electrospray ionization mass spectrometry (LC-ESI-MS) was developed to systematically analyze and identify the steroidal glycosides in four major <i>Polygonatum</i> species distributed in Japan, including <i>P. odoratum</i>, <i>P. falcatum</i>, <i>P. macranthum</i>, and <i>P. sibiricum</i>. As a result, 31 steroidal glycosides were tentatively identified, including 18 known and 13 previously unreported glycosides. Their structures were identified by the interpretation of chromatographic behavior and ESI-MS fragmentation patterns. The identification of 31 steroidal glycosides was indicative of a common biogenetic pathway in <i>Polygonatum</i> species. Our study disclosed the chemical profiling of steroidal glycosides in the plants of <i>Polygonatum</i> species, which will benefit better phytochemotaxonomical and phytochemical understanding and quality control for their medicinal usage. |
| first_indexed | 2024-03-09T11:34:56Z |
| format | Article |
| id | doaj.art-6abda27a02ec4e7598b83266af4fed76 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T11:34:56Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6abda27a02ec4e7598b83266af4fed762023-11-30T23:43:37ZengMDPI AGMolecules1420-30492023-01-0128270510.3390/molecules28020705Characterization and Comparison of Steroidal Glycosides from <i>Polygonatum</i> Species by High-Performance Liquid Chromatography–Electrospray Ionization Mass SpectrometryDanyang Liu0Takashi Kikuchi1Wei Li2Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, Chiba, JapanFaculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, Chiba, JapanFaculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, Chiba, Japan<i>Polygonatum</i> species have been used as traditional medicines and functional foods in Asia and Europe since ancient times. In this study, a fast and simple method based on liquid chromatography coupled with electrospray ionization mass spectrometry (LC-ESI-MS) was developed to systematically analyze and identify the steroidal glycosides in four major <i>Polygonatum</i> species distributed in Japan, including <i>P. odoratum</i>, <i>P. falcatum</i>, <i>P. macranthum</i>, and <i>P. sibiricum</i>. As a result, 31 steroidal glycosides were tentatively identified, including 18 known and 13 previously unreported glycosides. Their structures were identified by the interpretation of chromatographic behavior and ESI-MS fragmentation patterns. The identification of 31 steroidal glycosides was indicative of a common biogenetic pathway in <i>Polygonatum</i> species. Our study disclosed the chemical profiling of steroidal glycosides in the plants of <i>Polygonatum</i> species, which will benefit better phytochemotaxonomical and phytochemical understanding and quality control for their medicinal usage.https://www.mdpi.com/1420-3049/28/2/705HPLC-ESI-MS<i>Polygonatum</i> speciessteroidal glycosides |
| spellingShingle | Danyang Liu Takashi Kikuchi Wei Li Characterization and Comparison of Steroidal Glycosides from <i>Polygonatum</i> Species by High-Performance Liquid Chromatography–Electrospray Ionization Mass Spectrometry Molecules HPLC-ESI-MS <i>Polygonatum</i> species steroidal glycosides |
| title | Characterization and Comparison of Steroidal Glycosides from <i>Polygonatum</i> Species by High-Performance Liquid Chromatography–Electrospray Ionization Mass Spectrometry |
| title_full | Characterization and Comparison of Steroidal Glycosides from <i>Polygonatum</i> Species by High-Performance Liquid Chromatography–Electrospray Ionization Mass Spectrometry |
| title_fullStr | Characterization and Comparison of Steroidal Glycosides from <i>Polygonatum</i> Species by High-Performance Liquid Chromatography–Electrospray Ionization Mass Spectrometry |
| title_full_unstemmed | Characterization and Comparison of Steroidal Glycosides from <i>Polygonatum</i> Species by High-Performance Liquid Chromatography–Electrospray Ionization Mass Spectrometry |
| title_short | Characterization and Comparison of Steroidal Glycosides from <i>Polygonatum</i> Species by High-Performance Liquid Chromatography–Electrospray Ionization Mass Spectrometry |
| title_sort | characterization and comparison of steroidal glycosides from i polygonatum i species by high performance liquid chromatography electrospray ionization mass spectrometry |
| topic | HPLC-ESI-MS <i>Polygonatum</i> species steroidal glycosides |
| url | https://www.mdpi.com/1420-3049/28/2/705 |
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