Evaluation of the anti-oxidant effect of ascorbic acid on apoptosis and proliferation of germinal epithelium cells of rat testis following malathion-induced toxicity
<em><strong>Objective(s):</strong></em> The aim of this study was to determine the protective role of ascorbic acid on apoptosis and proliferation of spermatogonia and primary spermatocyte cells after malathion administration as an organophosphate pesticide in rat testis.<...
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| Format: | Article |
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Mashhad University of Medical Sciences
2020-05-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_15197_a5800745ded60349e8efaa480e17158c.pdf |
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| author | Faezeh Ghorbani-Taherdehi Mohammad Reza Nikravesh Mahdi Jalali Alireza Fazel Mahmoud Gorji Valokola |
| author_facet | Faezeh Ghorbani-Taherdehi Mohammad Reza Nikravesh Mahdi Jalali Alireza Fazel Mahmoud Gorji Valokola |
| author_sort | Faezeh Ghorbani-Taherdehi |
| collection | DOAJ |
| description | <em><strong>Objective(s):</strong></em> The aim of this study was to determine the protective role of ascorbic acid on apoptosis and proliferation of spermatogonia and primary spermatocyte cells after malathion administration as an organophosphate pesticide in rat testis.<br /><em><strong>Materials and Methods:</strong></em> Thirty male Wistar rats were randomly divided into five groups of 6 rats each, including control (no intervention), sham (normal saline 0.09%), malathion (50 mg/kg), malathion plus ascorbic acid (50 mg/kg and 200 mg/kg, respectively), and ascorbic acid (200 mg/kg) groups. Malathion and ascorbic acid were administrated via intraperitoneal injection once per day and seven times per week. After 6 weeks, animals were sacrificed, and testis tissue was used for evaluation of apoptosis and proliferation of germinal epithelium cells using the TUNEL and PCNA staining techniques. <br /><em><strong>Results:</strong></em> The results of TUNEL staining showed that the numbers of apoptotic cells in spermatogonia and primary spermatocyte cells were significantly increased in the malathion 50 mg/kg group vs control group (P<0.001). Co-administration of malathion 50 mg/kg and ascorbic acid 200 mg/kg significantly decreased the apoptotic cells in both cell types in comparison with malathion 50 mg/kg group (<em>P<</em>0.001). The results of PCNA staining revealed that the proliferation of these cells was significantly decreased in malathion 50 mg/kg group vs control group (<em>P<</em>0.001), and malathion 50 mg/kg plus ascorbic acid 200 mg/kg administration increased the proliferation of cells compared with malathion 50 mg/kg group (<em>P<</em>0.001).<br /><em><strong>Conclusion:</strong></em> The results provide evidence that ascorbic acid showed preventive effects on malathion-induced toxicity in male rat testis. |
| first_indexed | 2024-04-12T20:36:01Z |
| format | Article |
| id | doaj.art-6ac1d9373c82402fa7071dc2556bf2e0 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-12T20:36:01Z |
| publishDate | 2020-05-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-6ac1d9373c82402fa7071dc2556bf2e02022-12-22T03:17:34ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742020-05-0123556957510.22038/ijbms.2020.35952.860815197Evaluation of the anti-oxidant effect of ascorbic acid on apoptosis and proliferation of germinal epithelium cells of rat testis following malathion-induced toxicityFaezeh Ghorbani-Taherdehi0Mohammad Reza Nikravesh1Mahdi Jalali2Alireza Fazel3Mahmoud Gorji Valokola4Department of Anatomy and Cell Biology, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Anatomy and Cell Biology, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Anatomy and Cell Biology, Mashhad University of Medical Sciences, Mashhad, IranMicroanatomy Research Center, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran<em><strong>Objective(s):</strong></em> The aim of this study was to determine the protective role of ascorbic acid on apoptosis and proliferation of spermatogonia and primary spermatocyte cells after malathion administration as an organophosphate pesticide in rat testis.<br /><em><strong>Materials and Methods:</strong></em> Thirty male Wistar rats were randomly divided into five groups of 6 rats each, including control (no intervention), sham (normal saline 0.09%), malathion (50 mg/kg), malathion plus ascorbic acid (50 mg/kg and 200 mg/kg, respectively), and ascorbic acid (200 mg/kg) groups. Malathion and ascorbic acid were administrated via intraperitoneal injection once per day and seven times per week. After 6 weeks, animals were sacrificed, and testis tissue was used for evaluation of apoptosis and proliferation of germinal epithelium cells using the TUNEL and PCNA staining techniques. <br /><em><strong>Results:</strong></em> The results of TUNEL staining showed that the numbers of apoptotic cells in spermatogonia and primary spermatocyte cells were significantly increased in the malathion 50 mg/kg group vs control group (P<0.001). Co-administration of malathion 50 mg/kg and ascorbic acid 200 mg/kg significantly decreased the apoptotic cells in both cell types in comparison with malathion 50 mg/kg group (<em>P<</em>0.001). The results of PCNA staining revealed that the proliferation of these cells was significantly decreased in malathion 50 mg/kg group vs control group (<em>P<</em>0.001), and malathion 50 mg/kg plus ascorbic acid 200 mg/kg administration increased the proliferation of cells compared with malathion 50 mg/kg group (<em>P<</em>0.001).<br /><em><strong>Conclusion:</strong></em> The results provide evidence that ascorbic acid showed preventive effects on malathion-induced toxicity in male rat testis.http://ijbms.mums.ac.ir/article_15197_a5800745ded60349e8efaa480e17158c.pdfapoptosisascorbic acidmalathionpcnarattunel technique |
| spellingShingle | Faezeh Ghorbani-Taherdehi Mohammad Reza Nikravesh Mahdi Jalali Alireza Fazel Mahmoud Gorji Valokola Evaluation of the anti-oxidant effect of ascorbic acid on apoptosis and proliferation of germinal epithelium cells of rat testis following malathion-induced toxicity Iranian Journal of Basic Medical Sciences apoptosis ascorbic acid malathion pcna rat tunel technique |
| title | Evaluation of the anti-oxidant effect of ascorbic acid on apoptosis and proliferation of germinal epithelium cells of rat testis following malathion-induced toxicity |
| title_full | Evaluation of the anti-oxidant effect of ascorbic acid on apoptosis and proliferation of germinal epithelium cells of rat testis following malathion-induced toxicity |
| title_fullStr | Evaluation of the anti-oxidant effect of ascorbic acid on apoptosis and proliferation of germinal epithelium cells of rat testis following malathion-induced toxicity |
| title_full_unstemmed | Evaluation of the anti-oxidant effect of ascorbic acid on apoptosis and proliferation of germinal epithelium cells of rat testis following malathion-induced toxicity |
| title_short | Evaluation of the anti-oxidant effect of ascorbic acid on apoptosis and proliferation of germinal epithelium cells of rat testis following malathion-induced toxicity |
| title_sort | evaluation of the anti oxidant effect of ascorbic acid on apoptosis and proliferation of germinal epithelium cells of rat testis following malathion induced toxicity |
| topic | apoptosis ascorbic acid malathion pcna rat tunel technique |
| url | http://ijbms.mums.ac.ir/article_15197_a5800745ded60349e8efaa480e17158c.pdf |
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