Agmatine inhibits nuclear factor-κB nuclear translocation in acute spinal cord compression injury rat model
Secondary damage after acute spinal cord compression injury (SCCI) exacerbates initial insult. Nuclear factor kappa-B (NF-κB)-p65 activation is involved in SCCI deleterious effects. Agmatine (Agm) showed neuroprotection against various CNS injuries. However, Agm impact on NF-κB signaling in acute SC...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2016-09-01
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| Series: | Alexandria Journal of Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2090506815000706 |
| _version_ | 1818669509604540416 |
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| author | Doaa M. Samy Passainte S. Hassan Cherine A. Ismail Mona A. Hady Mohamed A. Eshra |
| author_facet | Doaa M. Samy Passainte S. Hassan Cherine A. Ismail Mona A. Hady Mohamed A. Eshra |
| author_sort | Doaa M. Samy |
| collection | DOAJ |
| description | Secondary damage after acute spinal cord compression injury (SCCI) exacerbates initial insult. Nuclear factor kappa-B (NF-κB)-p65 activation is involved in SCCI deleterious effects. Agmatine (Agm) showed neuroprotection against various CNS injuries. However, Agm impact on NF-κB signaling in acute SCCI remains to be investigated. The present study compared the effectiveness of Agm therapy and decompression laminectomy (DL) in functional recovery, oxidative stress, inflammatory and apoptotic responses, and modulation of NF-κB activation in acute SCCI rat model. Rats were either sham-operated or subjected to SCCI at T8–9, using 2-Fr. catheter. SCCI rats were randomly treated with DL at T8–9, intraperitoneal Agm (100 mg/kg/day), combined (DL/Agm) treatment or saline (n = 16/group). After 28-days of neurological follow-up, spinal cords were either subjected to biochemical measurement of oxidative stress and inflammatory markers or histopathology and immuno-histochemistry for NF-κB-p65 and caspase-3 expression (n = 8/group). Agm was comparable to DL in facilitating neurological functions recovery, reducing inflammation (TNF-α/interleukin-6), and apoptosis. Agm was distinctive in combating oxidative stress. Agm neuroprotective effects were paralleled with inhibition of NF-κB-p65 nuclear translocation. Combined pharmacological and surgical interventions were proved superior in functional recovery. In conclusion, present research suggested a new mechanism for Agm neuroprotection in rats SCCI through inhibition of NF-κB activation. |
| first_indexed | 2024-12-17T06:53:21Z |
| format | Article |
| id | doaj.art-6ac4b973b852473ebb47843c51a592a8 |
| institution | Directory Open Access Journal |
| issn | 2090-5068 |
| language | English |
| last_indexed | 2024-12-17T06:53:21Z |
| publishDate | 2016-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Alexandria Journal of Medicine |
| spelling | doaj.art-6ac4b973b852473ebb47843c51a592a82022-12-21T21:59:31ZengTaylor & Francis GroupAlexandria Journal of Medicine2090-50682016-09-0152325126010.1016/j.ajme.2015.09.001Agmatine inhibits nuclear factor-κB nuclear translocation in acute spinal cord compression injury rat modelDoaa M. Samy0Passainte S. Hassan1Cherine A. Ismail2Mona A. Hady3Mohamed A. Eshra4Department of Medical Physiology, Faculty of Medicine, Alexandria University, Alexandria, EgyptDepartment of Medical Physiology, Faculty of Medicine, Alexandria University, Alexandria, EgyptDepartment of Clinical Pharmacology, Faculty of Medicine, Alexandria University, Alexandria, EgyptDepartment of Pathology, Faculty of Medicine, Alexandria University, Alexandria, EgyptDepartment of Neurosurgery, Faculty of Medicine, Alexandria Main University Hospital, Alexandria, EgyptSecondary damage after acute spinal cord compression injury (SCCI) exacerbates initial insult. Nuclear factor kappa-B (NF-κB)-p65 activation is involved in SCCI deleterious effects. Agmatine (Agm) showed neuroprotection against various CNS injuries. However, Agm impact on NF-κB signaling in acute SCCI remains to be investigated. The present study compared the effectiveness of Agm therapy and decompression laminectomy (DL) in functional recovery, oxidative stress, inflammatory and apoptotic responses, and modulation of NF-κB activation in acute SCCI rat model. Rats were either sham-operated or subjected to SCCI at T8–9, using 2-Fr. catheter. SCCI rats were randomly treated with DL at T8–9, intraperitoneal Agm (100 mg/kg/day), combined (DL/Agm) treatment or saline (n = 16/group). After 28-days of neurological follow-up, spinal cords were either subjected to biochemical measurement of oxidative stress and inflammatory markers or histopathology and immuno-histochemistry for NF-κB-p65 and caspase-3 expression (n = 8/group). Agm was comparable to DL in facilitating neurological functions recovery, reducing inflammation (TNF-α/interleukin-6), and apoptosis. Agm was distinctive in combating oxidative stress. Agm neuroprotective effects were paralleled with inhibition of NF-κB-p65 nuclear translocation. Combined pharmacological and surgical interventions were proved superior in functional recovery. In conclusion, present research suggested a new mechanism for Agm neuroprotection in rats SCCI through inhibition of NF-κB activation.http://www.sciencedirect.com/science/article/pii/S2090506815000706Spinal cord injuryAgmatineNuclear factor kappa BOxidative stressInflammationApoptosis |
| spellingShingle | Doaa M. Samy Passainte S. Hassan Cherine A. Ismail Mona A. Hady Mohamed A. Eshra Agmatine inhibits nuclear factor-κB nuclear translocation in acute spinal cord compression injury rat model Alexandria Journal of Medicine Spinal cord injury Agmatine Nuclear factor kappa B Oxidative stress Inflammation Apoptosis |
| title | Agmatine inhibits nuclear factor-κB nuclear translocation in acute spinal cord compression injury rat model |
| title_full | Agmatine inhibits nuclear factor-κB nuclear translocation in acute spinal cord compression injury rat model |
| title_fullStr | Agmatine inhibits nuclear factor-κB nuclear translocation in acute spinal cord compression injury rat model |
| title_full_unstemmed | Agmatine inhibits nuclear factor-κB nuclear translocation in acute spinal cord compression injury rat model |
| title_short | Agmatine inhibits nuclear factor-κB nuclear translocation in acute spinal cord compression injury rat model |
| title_sort | agmatine inhibits nuclear factor κb nuclear translocation in acute spinal cord compression injury rat model |
| topic | Spinal cord injury Agmatine Nuclear factor kappa B Oxidative stress Inflammation Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S2090506815000706 |
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