GnRH I and II up-regulate MMP-26 expression through the JNK pathway in human cytotrophoblasts
<p>Abstract</p> <p>Background</p> <p>Matrix metalloproteinase-26 (MMP-26), one of the main mediators of extracellular matrix (ECM) degradation, has been shown to exist in trophoblasts of human placenta and to play a role in trophoblast cell invasion. However, little is...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-01-01
|
| Series: | Reproductive Biology and Endocrinology |
| Online Access: | http://www.rbej.com/content/8/1/5 |
| _version_ | 1818823471770107904 |
|---|---|
| author | Wu Xiao-qiu Li Yu-xia Cao Bin Liu Jing Wang Yan-ling |
| author_facet | Wu Xiao-qiu Li Yu-xia Cao Bin Liu Jing Wang Yan-ling |
| author_sort | Wu Xiao-qiu |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Matrix metalloproteinase-26 (MMP-26), one of the main mediators of extracellular matrix (ECM) degradation, has been shown to exist in trophoblasts of human placenta and to play a role in trophoblast cell invasion. However, little is known about the regulation of MMP-26 expression in human trophoblasts. Recently, gonadotropin-releasing hormone I (GnRH I) and GnRH II have been shown to regulate the expression of MMP-2, MMP-9/tissue inhibitor of metalloproteinases 1 (TIMP-1), and urokinase plasminogen activator (uPA)/plasminogen activator inhibitor (PAI) in human trophoblasts, suggesting that these two hormones may work as paracrine and/or autocrine regulators in modulating the activities of various protease systems at the feto-maternal interface. In this study, we determined the regulatory effects of GnRH I and GnRH II on the expression of MMP-26 in human immortalized cytotrophoblast-like cell line, B6Tert-1.</p> <p>Methods</p> <p>Real-time PCR was used to quantify mRNA levels of MMP-26 in human trophoblast-like cell line, B6Tert-1 and primary cultured cytotrophoblasts. Western blotting was used to characterize the expression of MMP-26 and the phosphorylation of c-Jun NH2-terminal kinase (JNK) and extracellular signal-regulated kinase 1/2 (ERK1/2) in B6Tert-1 cells after treatment with GnRH I and GnRH II.</p> <p>Results</p> <p>We found that GnRH I increased MMP-26 expression in B6Tert-1 cells after 12 h of treatment at both the mRNA and protein level, while GnRH II increased MMP-26 expression beginning at 3 h of treatment. Treatment of GnRH I at 1 nM resulted in maximal increase of MMP-26 mRNA and protein levels, whereas GnRH II treatment at a concentration of 100 nM was required to induce maximal increase in MMP-26 expression. In addition, we demonstrated that the activation of JNK, but not ERK1/2, was required for GnRH I and II-stimulated MMP-26 production in B6Tert-1 cells and primary cytotrophoblasts.</p> <p>Conclusions</p> <p>These novel findings indicated that GnRH I and II could up-regulate MMP-26 expression through the JNK signaling pathway in human trophoblast-like/trophoblast cells.</p> |
| first_indexed | 2024-12-18T23:40:31Z |
| format | Article |
| id | doaj.art-6ac721e67634438298b69e905fa7fac5 |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-12-18T23:40:31Z |
| publishDate | 2010-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-6ac721e67634438298b69e905fa7fac52022-12-21T20:47:24ZengBMCReproductive Biology and Endocrinology1477-78272010-01-0181510.1186/1477-7827-8-5GnRH I and II up-regulate MMP-26 expression through the JNK pathway in human cytotrophoblastsWu Xiao-qiuLi Yu-xiaCao BinLiu JingWang Yan-ling<p>Abstract</p> <p>Background</p> <p>Matrix metalloproteinase-26 (MMP-26), one of the main mediators of extracellular matrix (ECM) degradation, has been shown to exist in trophoblasts of human placenta and to play a role in trophoblast cell invasion. However, little is known about the regulation of MMP-26 expression in human trophoblasts. Recently, gonadotropin-releasing hormone I (GnRH I) and GnRH II have been shown to regulate the expression of MMP-2, MMP-9/tissue inhibitor of metalloproteinases 1 (TIMP-1), and urokinase plasminogen activator (uPA)/plasminogen activator inhibitor (PAI) in human trophoblasts, suggesting that these two hormones may work as paracrine and/or autocrine regulators in modulating the activities of various protease systems at the feto-maternal interface. In this study, we determined the regulatory effects of GnRH I and GnRH II on the expression of MMP-26 in human immortalized cytotrophoblast-like cell line, B6Tert-1.</p> <p>Methods</p> <p>Real-time PCR was used to quantify mRNA levels of MMP-26 in human trophoblast-like cell line, B6Tert-1 and primary cultured cytotrophoblasts. Western blotting was used to characterize the expression of MMP-26 and the phosphorylation of c-Jun NH2-terminal kinase (JNK) and extracellular signal-regulated kinase 1/2 (ERK1/2) in B6Tert-1 cells after treatment with GnRH I and GnRH II.</p> <p>Results</p> <p>We found that GnRH I increased MMP-26 expression in B6Tert-1 cells after 12 h of treatment at both the mRNA and protein level, while GnRH II increased MMP-26 expression beginning at 3 h of treatment. Treatment of GnRH I at 1 nM resulted in maximal increase of MMP-26 mRNA and protein levels, whereas GnRH II treatment at a concentration of 100 nM was required to induce maximal increase in MMP-26 expression. In addition, we demonstrated that the activation of JNK, but not ERK1/2, was required for GnRH I and II-stimulated MMP-26 production in B6Tert-1 cells and primary cytotrophoblasts.</p> <p>Conclusions</p> <p>These novel findings indicated that GnRH I and II could up-regulate MMP-26 expression through the JNK signaling pathway in human trophoblast-like/trophoblast cells.</p>http://www.rbej.com/content/8/1/5 |
| spellingShingle | Wu Xiao-qiu Li Yu-xia Cao Bin Liu Jing Wang Yan-ling GnRH I and II up-regulate MMP-26 expression through the JNK pathway in human cytotrophoblasts Reproductive Biology and Endocrinology |
| title | GnRH I and II up-regulate MMP-26 expression through the JNK pathway in human cytotrophoblasts |
| title_full | GnRH I and II up-regulate MMP-26 expression through the JNK pathway in human cytotrophoblasts |
| title_fullStr | GnRH I and II up-regulate MMP-26 expression through the JNK pathway in human cytotrophoblasts |
| title_full_unstemmed | GnRH I and II up-regulate MMP-26 expression through the JNK pathway in human cytotrophoblasts |
| title_short | GnRH I and II up-regulate MMP-26 expression through the JNK pathway in human cytotrophoblasts |
| title_sort | gnrh i and ii up regulate mmp 26 expression through the jnk pathway in human cytotrophoblasts |
| url | http://www.rbej.com/content/8/1/5 |
| work_keys_str_mv | AT wuxiaoqiu gnrhiandiiupregulatemmp26expressionthroughthejnkpathwayinhumancytotrophoblasts AT liyuxia gnrhiandiiupregulatemmp26expressionthroughthejnkpathwayinhumancytotrophoblasts AT caobin gnrhiandiiupregulatemmp26expressionthroughthejnkpathwayinhumancytotrophoblasts AT liujing gnrhiandiiupregulatemmp26expressionthroughthejnkpathwayinhumancytotrophoblasts AT wangyanling gnrhiandiiupregulatemmp26expressionthroughthejnkpathwayinhumancytotrophoblasts |