Establishment and Functional Characterization of Murine Monoclonal Antibodies Recognizing Neuritin
Neuritin represents a neurotrophic factor that is not only important in neuronal development and plasticity but also impacts endothelial angiogenesis, cell migration, tumor growth and the production of antibodies by B cells. We established monoclonal mouse anti-mouse neuritin antibodies by immunizin...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | Antibodies |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4468/12/2/28 |
| _version_ | 1797596387775348736 |
|---|---|
| author | Georgia Papadogianni Inga Ravens Ahmed Hassan Andrew Flatley Regina Feederle Günter Bernhardt Hristo Georgiev |
| author_facet | Georgia Papadogianni Inga Ravens Ahmed Hassan Andrew Flatley Regina Feederle Günter Bernhardt Hristo Georgiev |
| author_sort | Georgia Papadogianni |
| collection | DOAJ |
| description | Neuritin represents a neurotrophic factor that is not only important in neuronal development and plasticity but also impacts endothelial angiogenesis, cell migration, tumor growth and the production of antibodies by B cells. We established monoclonal mouse anti-mouse neuritin antibodies by immunizing knock-out mice with two different neuritin-derived peptides. Because neuritin is well conserved between species, these new monoclonal antibodies recognize the neuritin of a wide variety of species, including human. Moreover, they not only recognize specifically surface-bound neuritin expressed by murine follicular regulatory T cells but also the block binding of recombinant neuritin to germinal center B cells. This suggests that these newly generated tools will be of great use in studying neuritin expression and function. |
| first_indexed | 2024-03-11T02:50:40Z |
| format | Article |
| id | doaj.art-6ac788f9b6564733be1bd03bbf52e882 |
| institution | Directory Open Access Journal |
| issn | 2073-4468 |
| language | English |
| last_indexed | 2024-03-11T02:50:40Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibodies |
| spelling | doaj.art-6ac788f9b6564733be1bd03bbf52e8822023-11-18T09:01:48ZengMDPI AGAntibodies2073-44682023-04-011222810.3390/antib12020028Establishment and Functional Characterization of Murine Monoclonal Antibodies Recognizing NeuritinGeorgia Papadogianni0Inga Ravens1Ahmed Hassan2Andrew Flatley3Regina Feederle4Günter Bernhardt5Hristo Georgiev6Institute of Immunology, Hannover Medical School, 30625 Hannover, GermanyInstitute of Immunology, Hannover Medical School, 30625 Hannover, GermanyInstitute of Immunology, Hannover Medical School, 30625 Hannover, GermanyMonoclonal Antibody Core Facility, Helmholtz Center Munich, Research Center for Environmental Health (GmbH), 85764 Neuherberg, GermanyMonoclonal Antibody Core Facility, Helmholtz Center Munich, Research Center for Environmental Health (GmbH), 85764 Neuherberg, GermanyInstitute of Immunology, Hannover Medical School, 30625 Hannover, GermanyInstitute of Immunology, Hannover Medical School, 30625 Hannover, GermanyNeuritin represents a neurotrophic factor that is not only important in neuronal development and plasticity but also impacts endothelial angiogenesis, cell migration, tumor growth and the production of antibodies by B cells. We established monoclonal mouse anti-mouse neuritin antibodies by immunizing knock-out mice with two different neuritin-derived peptides. Because neuritin is well conserved between species, these new monoclonal antibodies recognize the neuritin of a wide variety of species, including human. Moreover, they not only recognize specifically surface-bound neuritin expressed by murine follicular regulatory T cells but also the block binding of recombinant neuritin to germinal center B cells. This suggests that these newly generated tools will be of great use in studying neuritin expression and function.https://www.mdpi.com/2073-4468/12/2/28neuritinblocking antibodyflow cytometryELISAmonoclonal antibody |
| spellingShingle | Georgia Papadogianni Inga Ravens Ahmed Hassan Andrew Flatley Regina Feederle Günter Bernhardt Hristo Georgiev Establishment and Functional Characterization of Murine Monoclonal Antibodies Recognizing Neuritin Antibodies neuritin blocking antibody flow cytometry ELISA monoclonal antibody |
| title | Establishment and Functional Characterization of Murine Monoclonal Antibodies Recognizing Neuritin |
| title_full | Establishment and Functional Characterization of Murine Monoclonal Antibodies Recognizing Neuritin |
| title_fullStr | Establishment and Functional Characterization of Murine Monoclonal Antibodies Recognizing Neuritin |
| title_full_unstemmed | Establishment and Functional Characterization of Murine Monoclonal Antibodies Recognizing Neuritin |
| title_short | Establishment and Functional Characterization of Murine Monoclonal Antibodies Recognizing Neuritin |
| title_sort | establishment and functional characterization of murine monoclonal antibodies recognizing neuritin |
| topic | neuritin blocking antibody flow cytometry ELISA monoclonal antibody |
| url | https://www.mdpi.com/2073-4468/12/2/28 |
| work_keys_str_mv | AT georgiapapadogianni establishmentandfunctionalcharacterizationofmurinemonoclonalantibodiesrecognizingneuritin AT ingaravens establishmentandfunctionalcharacterizationofmurinemonoclonalantibodiesrecognizingneuritin AT ahmedhassan establishmentandfunctionalcharacterizationofmurinemonoclonalantibodiesrecognizingneuritin AT andrewflatley establishmentandfunctionalcharacterizationofmurinemonoclonalantibodiesrecognizingneuritin AT reginafeederle establishmentandfunctionalcharacterizationofmurinemonoclonalantibodiesrecognizingneuritin AT gunterbernhardt establishmentandfunctionalcharacterizationofmurinemonoclonalantibodiesrecognizingneuritin AT hristogeorgiev establishmentandfunctionalcharacterizationofmurinemonoclonalantibodiesrecognizingneuritin |