A Novel Nutraceutical Formulation Can Improve Motor Activity and Decrease the Stress Level in a Murine Model of Middle-Age Animals
Ageing is a genetically programmed physiological process that is modulated by numerous environmental factors, associated with decreasing physiological function, decreasing reproductive rate and increasing age-related mortality rate. Maintaining mobility performance and physical function in the elder...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/10/4/624 |
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| author | Dimitris Tsoukalas Ovidiu Zlatian Mihaela Mitroi Elisavet Renieri Aristidis Tsatsakis Boris Nikolaevich Izotov Florin Burada Simona Sosoi Emilia Burada Ana Maria Buga Ion Rogoveanu Anca Oana Docea Daniela Calina |
| author_facet | Dimitris Tsoukalas Ovidiu Zlatian Mihaela Mitroi Elisavet Renieri Aristidis Tsatsakis Boris Nikolaevich Izotov Florin Burada Simona Sosoi Emilia Burada Ana Maria Buga Ion Rogoveanu Anca Oana Docea Daniela Calina |
| author_sort | Dimitris Tsoukalas |
| collection | DOAJ |
| description | Ageing is a genetically programmed physiological process that is modulated by numerous environmental factors, associated with decreasing physiological function, decreasing reproductive rate and increasing age-related mortality rate. Maintaining mobility performance and physical function in the elderly is the main objective of the successful ageing concept. In this study, we aimed to evaluate the beneficial effect of a novel nutraceutical formulation containing <i>Centella asiatica</i> L. extract, vitamin C, zinc and vitamin D3 (as cholecalciferol) on motor activity and anxiety with the use of a murine model of old animals, as a means of providing proof for clinical use in the elderly, for enhancing physical strength and improving life quality. Eighteen Sprague Dawley 18 months old male rats were divided into three groups and received corn oil (the control group) or 1 capsule/kg bw Reverse supplement (treatment group 1) or 2 capsules/kg bw Reverse supplement (treatment group 2), for a period of 3 months. The Reverse supplement (Natural Doctor S.A, Athens, Greece) contains 9 mg <i>Centella asiatica</i> L. extract, vitamin C (200 mg as magnesium ascorbate), zinc (5 mg as zinc citrate), vitamin D3 (50 µg as cholecalciferol) per capsule. Before and after the treatment, the motor function and behavioral changes for anxiety and depression were evaluated using the open-field test, elevated plus-maze test and rotarod test. The supplementation with Reverse (Natural Doctor S.A) supplement can improve the locomotor activity in old rats in a dose-dependent manner, as demonstrated by an increase in the latency to leave from the middle square, in the number of rearings in the open field test, in the time spent in the open arms and time spent in the center in the elevated plus-maze test and the latency to all in all three consecutive trials in the rotarod test. Stress also decreased significantly in a dose-dependent manner, following the treatment with Reverse supplement, as was demonstrated by the decrease in the number of groomings at the open field test and time spent in the dark and the number of groomings at the elevated plus-maze test. |
| first_indexed | 2024-03-09T05:20:09Z |
| format | Article |
| id | doaj.art-6ac8434d067a4868857935e32efc5513 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-09T05:20:09Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-6ac8434d067a4868857935e32efc55132023-12-03T12:41:34ZengMDPI AGJournal of Clinical Medicine2077-03832021-02-0110462410.3390/jcm10040624A Novel Nutraceutical Formulation Can Improve Motor Activity and Decrease the Stress Level in a Murine Model of Middle-Age AnimalsDimitris Tsoukalas0Ovidiu Zlatian1Mihaela Mitroi2Elisavet Renieri3Aristidis Tsatsakis4Boris Nikolaevich Izotov5Florin Burada6Simona Sosoi7Emilia Burada8Ana Maria Buga9Ion Rogoveanu10Anca Oana Docea11Daniela Calina12Department of Clinical Pharmacy, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Microbiology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaENT Department, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaLaboratory of Toxicology and Forensic Sciences, Medical School, University of Crete, 71003 Heraklion, GreeceLaboratory of Toxicology and Forensic Sciences, Medical School, University of Crete, 71003 Heraklion, GreeceDepartment of Analytical and Forensic Medical Toxicology, Sechenov University, 119991 Moscow, RussiaHuman Genomics Laboratory, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaHuman Genomics Laboratory, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Physiology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Biochemistry, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Internal Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Toxicology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Clinical Pharmacy, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaAgeing is a genetically programmed physiological process that is modulated by numerous environmental factors, associated with decreasing physiological function, decreasing reproductive rate and increasing age-related mortality rate. Maintaining mobility performance and physical function in the elderly is the main objective of the successful ageing concept. In this study, we aimed to evaluate the beneficial effect of a novel nutraceutical formulation containing <i>Centella asiatica</i> L. extract, vitamin C, zinc and vitamin D3 (as cholecalciferol) on motor activity and anxiety with the use of a murine model of old animals, as a means of providing proof for clinical use in the elderly, for enhancing physical strength and improving life quality. Eighteen Sprague Dawley 18 months old male rats were divided into three groups and received corn oil (the control group) or 1 capsule/kg bw Reverse supplement (treatment group 1) or 2 capsules/kg bw Reverse supplement (treatment group 2), for a period of 3 months. The Reverse supplement (Natural Doctor S.A, Athens, Greece) contains 9 mg <i>Centella asiatica</i> L. extract, vitamin C (200 mg as magnesium ascorbate), zinc (5 mg as zinc citrate), vitamin D3 (50 µg as cholecalciferol) per capsule. Before and after the treatment, the motor function and behavioral changes for anxiety and depression were evaluated using the open-field test, elevated plus-maze test and rotarod test. The supplementation with Reverse (Natural Doctor S.A) supplement can improve the locomotor activity in old rats in a dose-dependent manner, as demonstrated by an increase in the latency to leave from the middle square, in the number of rearings in the open field test, in the time spent in the open arms and time spent in the center in the elevated plus-maze test and the latency to all in all three consecutive trials in the rotarod test. Stress also decreased significantly in a dose-dependent manner, following the treatment with Reverse supplement, as was demonstrated by the decrease in the number of groomings at the open field test and time spent in the dark and the number of groomings at the elevated plus-maze test.https://www.mdpi.com/2077-0383/10/4/624<i>Centella asiatica</i> L.vitamin Czincvitamin Danxiety levelsmotor performance |
| spellingShingle | Dimitris Tsoukalas Ovidiu Zlatian Mihaela Mitroi Elisavet Renieri Aristidis Tsatsakis Boris Nikolaevich Izotov Florin Burada Simona Sosoi Emilia Burada Ana Maria Buga Ion Rogoveanu Anca Oana Docea Daniela Calina A Novel Nutraceutical Formulation Can Improve Motor Activity and Decrease the Stress Level in a Murine Model of Middle-Age Animals Journal of Clinical Medicine <i>Centella asiatica</i> L. vitamin C zinc vitamin D anxiety levels motor performance |
| title | A Novel Nutraceutical Formulation Can Improve Motor Activity and Decrease the Stress Level in a Murine Model of Middle-Age Animals |
| title_full | A Novel Nutraceutical Formulation Can Improve Motor Activity and Decrease the Stress Level in a Murine Model of Middle-Age Animals |
| title_fullStr | A Novel Nutraceutical Formulation Can Improve Motor Activity and Decrease the Stress Level in a Murine Model of Middle-Age Animals |
| title_full_unstemmed | A Novel Nutraceutical Formulation Can Improve Motor Activity and Decrease the Stress Level in a Murine Model of Middle-Age Animals |
| title_short | A Novel Nutraceutical Formulation Can Improve Motor Activity and Decrease the Stress Level in a Murine Model of Middle-Age Animals |
| title_sort | novel nutraceutical formulation can improve motor activity and decrease the stress level in a murine model of middle age animals |
| topic | <i>Centella asiatica</i> L. vitamin C zinc vitamin D anxiety levels motor performance |
| url | https://www.mdpi.com/2077-0383/10/4/624 |
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