Effect of Polycyclic Aromatic Hydrocarbons Exposure on Sperm DNA in Idiopathic Male Infertility
Background. Biological mechanisms contribute to the relationship between polycyclic aromatic hydrocarbon (PAH) exposure and infertility in males by altering semen quality. Objectives. The aim of the present study was to evaluate the impact of PAHs on male infertility using the sperm chromatin disper...
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| Format: | Article |
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Pure Earth
2019-01-01
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| Series: | Journal of Health and Pollution |
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| _version_ | 1818338045123887104 |
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| author | Aziza A. Saad Tarek Hussein Amany El-Sikaily Mohamed A. Abdel-Mohsen El-Hassan Mokhamer Amany I. Youssef Jihan Mohammed |
| author_facet | Aziza A. Saad Tarek Hussein Amany El-Sikaily Mohamed A. Abdel-Mohsen El-Hassan Mokhamer Amany I. Youssef Jihan Mohammed |
| author_sort | Aziza A. Saad |
| collection | DOAJ |
| description | Background. Biological mechanisms contribute to the relationship between polycyclic aromatic hydrocarbon (PAH) exposure and infertility in males by altering semen quality.
Objectives. The aim of the present study was to evaluate the impact of PAHs on male infertility using the sperm chromatin dispersion test (Halo sperm assay).
Methods. Sixty-six (66) infertile males under 45 years of age were examined for the determination of urinary metabolite and oxidative stress by measuring lipid peroxidation and antioxidant activity of glutathione and glutathione-s-transferase, as well as hormonal activity of follicle stimulating hormone (FSH), testosterone and prolactin and semen quality.
Results. There was an increased level of urinary metabolite of 1-hydroxy pyrene, 1-hydroxy naphthalene and 2-hydroxy naphthalene in the urine of the infertile group. In addition, elevated concentrations of malondialdehyde coincided with a decreased level of antioxidants, leading to oxidative stress in the infertile group. Semen samples showed 30% sperm deoxyribonucleic acid (DNA) fragmentation.
Conclusions. The data provide strong evidence of a statistical threshold for semen samples containing 30% sperm DNA fragmentation resulting in a reduced level of pregnancy success.
Participant consent. Obtained
Ethics Approval. Study approval was given by the ethics committee of Alexandria University (United States Department of Health and Human Services, institutional review board registration (IRB), IORG0008812 Medical Research Institute, expires 4/8/2019, OMB No: 0990-0279).
Competing Interests. The authors declare no competing financial interests. |
| first_indexed | 2024-12-13T15:04:52Z |
| format | Article |
| id | doaj.art-6ac9bcc7236e491faeb982b8e620d0cd |
| institution | Directory Open Access Journal |
| issn | 2156-9614 2156-9614 |
| language | English |
| last_indexed | 2024-12-13T15:04:52Z |
| publishDate | 2019-01-01 |
| publisher | Pure Earth |
| record_format | Article |
| series | Journal of Health and Pollution |
| spelling | doaj.art-6ac9bcc7236e491faeb982b8e620d0cd2022-12-21T23:41:02ZengPure EarthJournal of Health and Pollution2156-96142156-96142019-01-0192111110.5696/2156-9614-9.21.1903092156-9614-9-21-190309Effect of Polycyclic Aromatic Hydrocarbons Exposure on Sperm DNA in Idiopathic Male InfertilityAziza A. Saad0Tarek Hussein1Amany El-Sikaily2Mohamed A. Abdel-Mohsen3El-Hassan Mokhamer4Amany I. Youssef5Jihan Mohammed6Applied Medical Chemistry, Medical Research Institute, Alexandria University, Alexandria, EgyptDermatology, Venereology and Andrology Department, Faculty of Medicine, Alexandria University, Alexandria, EgyptMarine Pollution Department, Marine Environment Division, National Institute of Oceanography and Fisheries, Alexandria, EgyptApplied Medical Chemistry, Medical Research Institute, Alexandria University, Alexandria, EgyptMolecular Biology lecturer, Zoology Department, Damnhour University, Damanhur, EgyptApplied Medical Chemistry, Medical Research Institute, Alexandria University, Alexandria, EgyptApplied Medical Chemistry, Medical Research Institute, Alexandria University, Alexandria, EgyptBackground. Biological mechanisms contribute to the relationship between polycyclic aromatic hydrocarbon (PAH) exposure and infertility in males by altering semen quality. Objectives. The aim of the present study was to evaluate the impact of PAHs on male infertility using the sperm chromatin dispersion test (Halo sperm assay). Methods. Sixty-six (66) infertile males under 45 years of age were examined for the determination of urinary metabolite and oxidative stress by measuring lipid peroxidation and antioxidant activity of glutathione and glutathione-s-transferase, as well as hormonal activity of follicle stimulating hormone (FSH), testosterone and prolactin and semen quality. Results. There was an increased level of urinary metabolite of 1-hydroxy pyrene, 1-hydroxy naphthalene and 2-hydroxy naphthalene in the urine of the infertile group. In addition, elevated concentrations of malondialdehyde coincided with a decreased level of antioxidants, leading to oxidative stress in the infertile group. Semen samples showed 30% sperm deoxyribonucleic acid (DNA) fragmentation. Conclusions. The data provide strong evidence of a statistical threshold for semen samples containing 30% sperm DNA fragmentation resulting in a reduced level of pregnancy success. Participant consent. Obtained Ethics Approval. Study approval was given by the ethics committee of Alexandria University (United States Department of Health and Human Services, institutional review board registration (IRB), IORG0008812 Medical Research Institute, expires 4/8/2019, OMB No: 0990-0279). Competing Interests. The authors declare no competing financial interests.PAHurinary metabolitesperm DNA fragmentationsemen |
| spellingShingle | Aziza A. Saad Tarek Hussein Amany El-Sikaily Mohamed A. Abdel-Mohsen El-Hassan Mokhamer Amany I. Youssef Jihan Mohammed Effect of Polycyclic Aromatic Hydrocarbons Exposure on Sperm DNA in Idiopathic Male Infertility Journal of Health and Pollution PAH urinary metabolite sperm DNA fragmentation semen |
| title | Effect of Polycyclic Aromatic Hydrocarbons Exposure on Sperm DNA in Idiopathic Male Infertility |
| title_full | Effect of Polycyclic Aromatic Hydrocarbons Exposure on Sperm DNA in Idiopathic Male Infertility |
| title_fullStr | Effect of Polycyclic Aromatic Hydrocarbons Exposure on Sperm DNA in Idiopathic Male Infertility |
| title_full_unstemmed | Effect of Polycyclic Aromatic Hydrocarbons Exposure on Sperm DNA in Idiopathic Male Infertility |
| title_short | Effect of Polycyclic Aromatic Hydrocarbons Exposure on Sperm DNA in Idiopathic Male Infertility |
| title_sort | effect of polycyclic aromatic hydrocarbons exposure on sperm dna in idiopathic male infertility |
| topic | PAH urinary metabolite sperm DNA fragmentation semen |
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