Insights into metazoan evolution from <it>alvinella pompejana </it>cDNAs
<p>Abstract</p> <p>Background</p> <p><it>Alvinella pompejana </it>is a representative of Annelids, a key phylum for evo-devo studies that is still poorly studied at the sequence level. <it>A. pompejana </it>inhabits deep-sea hydrothermal vents an...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-11-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/11/634 |
| _version_ | 1818385938329370624 |
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| author | Thierry Jean-Claude Tanguy Arnaud Shillito Bruce Segurens Béatrice Rees Jean-François Perrodou Emmanuel Moras Dino Mary Jean Leize-Wagner Emmanuelle Lallier François Hourdez Stéphane Knoops Bernard Gaill Françoise Higuet Dominique Da Silva Corinne Busso Didier Brélivet Yann Jollivet Didier Boutet Isabelle Gagnière Nicolas Weissenbach Jean Wincker Patrick Zal Franck Poch Olivier Lecompte Odile |
| author_facet | Thierry Jean-Claude Tanguy Arnaud Shillito Bruce Segurens Béatrice Rees Jean-François Perrodou Emmanuel Moras Dino Mary Jean Leize-Wagner Emmanuelle Lallier François Hourdez Stéphane Knoops Bernard Gaill Françoise Higuet Dominique Da Silva Corinne Busso Didier Brélivet Yann Jollivet Didier Boutet Isabelle Gagnière Nicolas Weissenbach Jean Wincker Patrick Zal Franck Poch Olivier Lecompte Odile |
| author_sort | Thierry Jean-Claude |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Alvinella pompejana </it>is a representative of Annelids, a key phylum for evo-devo studies that is still poorly studied at the sequence level. <it>A. pompejana </it>inhabits deep-sea hydrothermal vents and is currently known as one of the most thermotolerant Eukaryotes in marine environments, withstanding the largest known chemical and thermal ranges (from 5 to 105°C). This tube-dwelling worm forms dense colonies on the surface of hydrothermal chimneys and can withstand long periods of hypo/anoxia and long phases of exposure to hydrogen sulphides. <it>A. pompejana </it>specifically inhabits chimney walls of hydrothermal vents on the East Pacific Rise. To survive, <it>Alvinella </it>has developed numerous adaptations at the physiological and molecular levels, such as an increase in the thermostability of proteins and protein complexes. It represents an outstanding model organism for studying adaptation to harsh physicochemical conditions and for isolating stable macromolecules resistant to high temperatures.</p> <p>Results</p> <p>We have constructed four full length enriched cDNA libraries to investigate the biology and evolution of this intriguing animal. Analysis of more than 75,000 high quality reads led to the identification of 15,858 transcripts and 9,221 putative protein sequences. Our annotation reveals a good coverage of most animal pathways and networks with a prevalence of transcripts involved in oxidative stress resistance, detoxification, anti-bacterial defence, and heat shock protection. <it>Alvinella </it>proteins seem to show a slow evolutionary rate and a higher similarity with proteins from Vertebrates compared to proteins from Arthropods or Nematodes. Their composition shows enrichment in positively charged amino acids that might contribute to their thermostability. The gene content of <it>Alvinella </it>reveals that an important pool of genes previously considered to be specific to Deuterostomes were in fact already present in the last common ancestor of the Bilaterian animals, but have been secondarily lost in model invertebrates. This pool is enriched in glycoproteins that play a key role in intercellular communication, hormonal regulation and immunity.</p> <p>Conclusions</p> <p>Our study starts to unravel the gene content and sequence evolution of a deep-sea annelid, revealing key features in eukaryote adaptation to extreme environmental conditions and highlighting the proximity of Annelids and Vertebrates.</p> |
| first_indexed | 2024-12-14T03:46:06Z |
| format | Article |
| id | doaj.art-6ac9f552ed9c4b68afbabe06b641e371 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-14T03:46:06Z |
| publishDate | 2010-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-6ac9f552ed9c4b68afbabe06b641e3712022-12-21T23:18:21ZengBMCBMC Genomics1471-21642010-11-0111163410.1186/1471-2164-11-634Insights into metazoan evolution from <it>alvinella pompejana </it>cDNAsThierry Jean-ClaudeTanguy ArnaudShillito BruceSegurens BéatriceRees Jean-FrançoisPerrodou EmmanuelMoras DinoMary JeanLeize-Wagner EmmanuelleLallier FrançoisHourdez StéphaneKnoops BernardGaill FrançoiseHiguet DominiqueDa Silva CorinneBusso DidierBrélivet YannJollivet DidierBoutet IsabelleGagnière NicolasWeissenbach JeanWincker PatrickZal FranckPoch OlivierLecompte Odile<p>Abstract</p> <p>Background</p> <p><it>Alvinella pompejana </it>is a representative of Annelids, a key phylum for evo-devo studies that is still poorly studied at the sequence level. <it>A. pompejana </it>inhabits deep-sea hydrothermal vents and is currently known as one of the most thermotolerant Eukaryotes in marine environments, withstanding the largest known chemical and thermal ranges (from 5 to 105°C). This tube-dwelling worm forms dense colonies on the surface of hydrothermal chimneys and can withstand long periods of hypo/anoxia and long phases of exposure to hydrogen sulphides. <it>A. pompejana </it>specifically inhabits chimney walls of hydrothermal vents on the East Pacific Rise. To survive, <it>Alvinella </it>has developed numerous adaptations at the physiological and molecular levels, such as an increase in the thermostability of proteins and protein complexes. It represents an outstanding model organism for studying adaptation to harsh physicochemical conditions and for isolating stable macromolecules resistant to high temperatures.</p> <p>Results</p> <p>We have constructed four full length enriched cDNA libraries to investigate the biology and evolution of this intriguing animal. Analysis of more than 75,000 high quality reads led to the identification of 15,858 transcripts and 9,221 putative protein sequences. Our annotation reveals a good coverage of most animal pathways and networks with a prevalence of transcripts involved in oxidative stress resistance, detoxification, anti-bacterial defence, and heat shock protection. <it>Alvinella </it>proteins seem to show a slow evolutionary rate and a higher similarity with proteins from Vertebrates compared to proteins from Arthropods or Nematodes. Their composition shows enrichment in positively charged amino acids that might contribute to their thermostability. The gene content of <it>Alvinella </it>reveals that an important pool of genes previously considered to be specific to Deuterostomes were in fact already present in the last common ancestor of the Bilaterian animals, but have been secondarily lost in model invertebrates. This pool is enriched in glycoproteins that play a key role in intercellular communication, hormonal regulation and immunity.</p> <p>Conclusions</p> <p>Our study starts to unravel the gene content and sequence evolution of a deep-sea annelid, revealing key features in eukaryote adaptation to extreme environmental conditions and highlighting the proximity of Annelids and Vertebrates.</p>http://www.biomedcentral.com/1471-2164/11/634 |
| spellingShingle | Thierry Jean-Claude Tanguy Arnaud Shillito Bruce Segurens Béatrice Rees Jean-François Perrodou Emmanuel Moras Dino Mary Jean Leize-Wagner Emmanuelle Lallier François Hourdez Stéphane Knoops Bernard Gaill Françoise Higuet Dominique Da Silva Corinne Busso Didier Brélivet Yann Jollivet Didier Boutet Isabelle Gagnière Nicolas Weissenbach Jean Wincker Patrick Zal Franck Poch Olivier Lecompte Odile Insights into metazoan evolution from <it>alvinella pompejana </it>cDNAs BMC Genomics |
| title | Insights into metazoan evolution from <it>alvinella pompejana </it>cDNAs |
| title_full | Insights into metazoan evolution from <it>alvinella pompejana </it>cDNAs |
| title_fullStr | Insights into metazoan evolution from <it>alvinella pompejana </it>cDNAs |
| title_full_unstemmed | Insights into metazoan evolution from <it>alvinella pompejana </it>cDNAs |
| title_short | Insights into metazoan evolution from <it>alvinella pompejana </it>cDNAs |
| title_sort | insights into metazoan evolution from it alvinella pompejana it cdnas |
| url | http://www.biomedcentral.com/1471-2164/11/634 |
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