Rodent Models of Spondyloarthritis Have Decreased White and Bone Marrow Adipose Tissue Depots
Bone marrow adipose tissue (BMAT) has recently been recognized as a distinct fat depot with endocrine functions. However, if and how it is regulated by chronic inflammation remains unknown. Here, we investigate the amount of white fat and BMAT in HLA-B27 transgenic rats and curdlan-challenged SKG mi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.665208/full |
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| author | Giulia Furesi Ingrid Fert Ingrid Fert Ingrid Fert Marie Beaufrère Marie Beaufrère Marie Beaufrère Luiza M. Araujo Luiza M. Araujo Luiza M. Araujo Simon Glatigny Simon Glatigny Simon Glatigny Ulrike Baschant Malte von Bonin Lorenz C. Hofbauer Nicole J. Horwood Maxime Breban Maxime Breban Maxime Breban Martina Rauner |
| author_facet | Giulia Furesi Ingrid Fert Ingrid Fert Ingrid Fert Marie Beaufrère Marie Beaufrère Marie Beaufrère Luiza M. Araujo Luiza M. Araujo Luiza M. Araujo Simon Glatigny Simon Glatigny Simon Glatigny Ulrike Baschant Malte von Bonin Lorenz C. Hofbauer Nicole J. Horwood Maxime Breban Maxime Breban Maxime Breban Martina Rauner |
| author_sort | Giulia Furesi |
| collection | DOAJ |
| description | Bone marrow adipose tissue (BMAT) has recently been recognized as a distinct fat depot with endocrine functions. However, if and how it is regulated by chronic inflammation remains unknown. Here, we investigate the amount of white fat and BMAT in HLA-B27 transgenic rats and curdlan-challenged SKG mice, two well-established models of chronic inflammatory spondyloarthritis (SpA). Subcutaneous and gonadal white adipose tissue and BMAT was reduced by 65-70% and by up to 90% in both experimental models. Consistently, B27 rats had a 2-3-fold decrease in the serum concentrations of the adipocyte-derived cytokines adiponectin and leptin as well as a 2-fold lower concentration of triglycerides. The bone marrow of B27 rats was further characterized by higher numbers of neutrophils, lower numbers of erythroblast precursors, and higher numbers of IL-17 producing CD4+ T cells. IL-17 concentration was also increased in the serum of B27 rats. Using a cell culture model, we show that high levels of IL-17 in the serum of B27 rats negatively impacted adipogenesis (-76%), an effect that was reversed in the presence of neutralizing anti-IL-17 antibody. In summary, these findings show BMAT is severely reduced in two experimental models of chronic inflammatory SpA and suggest that IL-17 is involved in this process. |
| first_indexed | 2024-12-22T15:35:51Z |
| format | Article |
| id | doaj.art-6aca9b998d074ef8b43c5c6fe40f1150 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-22T15:35:51Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-6aca9b998d074ef8b43c5c6fe40f11502022-12-21T18:21:16ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-06-011210.3389/fimmu.2021.665208665208Rodent Models of Spondyloarthritis Have Decreased White and Bone Marrow Adipose Tissue DepotsGiulia Furesi0Ingrid Fert1Ingrid Fert2Ingrid Fert3Marie Beaufrère4Marie Beaufrère5Marie Beaufrère6Luiza M. Araujo7Luiza M. Araujo8Luiza M. Araujo9Simon Glatigny10Simon Glatigny11Simon Glatigny12Ulrike Baschant13Malte von Bonin14Lorenz C. Hofbauer15Nicole J. Horwood16Maxime Breban17Maxime Breban18Maxime Breban19Martina Rauner20Department of Medicine III & Center for Healthy Aging, Technische Universität Dresden, Dresden, GermanyLaboratoire Infection et inflammation, UMR U1173 INSERM/Université de Versailles-Saint-Quentin-Paris-Saclay, Montigny-le-Bretonneux, FranceLaboratoire d’Excellence Inflamex, Université Paris Descartes, Paris, FranceService de Rhumatologie, Hôpital Ambroise Paré, AP-HP, Boulogne, FranceLaboratoire Infection et inflammation, UMR U1173 INSERM/Université de Versailles-Saint-Quentin-Paris-Saclay, Montigny-le-Bretonneux, FranceLaboratoire d’Excellence Inflamex, Université Paris Descartes, Paris, FranceService de Rhumatologie, Hôpital Ambroise Paré, AP-HP, Boulogne, FranceLaboratoire Infection et inflammation, UMR U1173 INSERM/Université de Versailles-Saint-Quentin-Paris-Saclay, Montigny-le-Bretonneux, FranceLaboratoire d’Excellence Inflamex, Université Paris Descartes, Paris, FranceService de Rhumatologie, Hôpital Ambroise Paré, AP-HP, Boulogne, FranceLaboratoire Infection et inflammation, UMR U1173 INSERM/Université de Versailles-Saint-Quentin-Paris-Saclay, Montigny-le-Bretonneux, FranceLaboratoire d’Excellence Inflamex, Université Paris Descartes, Paris, FranceService de Rhumatologie, Hôpital Ambroise Paré, AP-HP, Boulogne, FranceDepartment of Medicine III & Center for Healthy Aging, Technische Universität Dresden, Dresden, GermanyDepartment of Medicine I, Technische Universität Dresden, Dresden, GermanyDepartment of Medicine III & Center for Healthy Aging, Technische Universität Dresden, Dresden, GermanyNorwich Medical School, University of East Anglia, Norwich, United KingdomLaboratoire Infection et inflammation, UMR U1173 INSERM/Université de Versailles-Saint-Quentin-Paris-Saclay, Montigny-le-Bretonneux, FranceLaboratoire d’Excellence Inflamex, Université Paris Descartes, Paris, FranceService de Rhumatologie, Hôpital Ambroise Paré, AP-HP, Boulogne, FranceDepartment of Medicine III & Center for Healthy Aging, Technische Universität Dresden, Dresden, GermanyBone marrow adipose tissue (BMAT) has recently been recognized as a distinct fat depot with endocrine functions. However, if and how it is regulated by chronic inflammation remains unknown. Here, we investigate the amount of white fat and BMAT in HLA-B27 transgenic rats and curdlan-challenged SKG mice, two well-established models of chronic inflammatory spondyloarthritis (SpA). Subcutaneous and gonadal white adipose tissue and BMAT was reduced by 65-70% and by up to 90% in both experimental models. Consistently, B27 rats had a 2-3-fold decrease in the serum concentrations of the adipocyte-derived cytokines adiponectin and leptin as well as a 2-fold lower concentration of triglycerides. The bone marrow of B27 rats was further characterized by higher numbers of neutrophils, lower numbers of erythroblast precursors, and higher numbers of IL-17 producing CD4+ T cells. IL-17 concentration was also increased in the serum of B27 rats. Using a cell culture model, we show that high levels of IL-17 in the serum of B27 rats negatively impacted adipogenesis (-76%), an effect that was reversed in the presence of neutralizing anti-IL-17 antibody. In summary, these findings show BMAT is severely reduced in two experimental models of chronic inflammatory SpA and suggest that IL-17 is involved in this process.https://www.frontiersin.org/articles/10.3389/fimmu.2021.665208/fullspondyloarthritisHLA-B27 transgenic ratSKG mousebone marrow fatIL-17 |
| spellingShingle | Giulia Furesi Ingrid Fert Ingrid Fert Ingrid Fert Marie Beaufrère Marie Beaufrère Marie Beaufrère Luiza M. Araujo Luiza M. Araujo Luiza M. Araujo Simon Glatigny Simon Glatigny Simon Glatigny Ulrike Baschant Malte von Bonin Lorenz C. Hofbauer Nicole J. Horwood Maxime Breban Maxime Breban Maxime Breban Martina Rauner Rodent Models of Spondyloarthritis Have Decreased White and Bone Marrow Adipose Tissue Depots Frontiers in Immunology spondyloarthritis HLA-B27 transgenic rat SKG mouse bone marrow fat IL-17 |
| title | Rodent Models of Spondyloarthritis Have Decreased White and Bone Marrow Adipose Tissue Depots |
| title_full | Rodent Models of Spondyloarthritis Have Decreased White and Bone Marrow Adipose Tissue Depots |
| title_fullStr | Rodent Models of Spondyloarthritis Have Decreased White and Bone Marrow Adipose Tissue Depots |
| title_full_unstemmed | Rodent Models of Spondyloarthritis Have Decreased White and Bone Marrow Adipose Tissue Depots |
| title_short | Rodent Models of Spondyloarthritis Have Decreased White and Bone Marrow Adipose Tissue Depots |
| title_sort | rodent models of spondyloarthritis have decreased white and bone marrow adipose tissue depots |
| topic | spondyloarthritis HLA-B27 transgenic rat SKG mouse bone marrow fat IL-17 |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.665208/full |
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