Development, physicochemical characterization and in-vitro biocompatibility study of dromedary camel dentine derived hydroxyapatite for bone repair
This study aimed to produce hydroxyapatite from the dentine portion of camel teeth using a defatting and deproteinizing procedure and characterize its physicochemical and biocompatibility properties. Biowaste such as waste camel teeth is a valuable source of hydroxyapatite, the main inorganic consti...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2023-08-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/15711.pdf |
| _version_ | 1797425823814254592 |
|---|---|
| author | Zohaib Khurshid Mohammed Farhan A. Alfarhan Yasmin Bayan Javed Mazher Necdet Adanir George J. Dias Paul R. Cooper Jithendra Ratnayake |
| author_facet | Zohaib Khurshid Mohammed Farhan A. Alfarhan Yasmin Bayan Javed Mazher Necdet Adanir George J. Dias Paul R. Cooper Jithendra Ratnayake |
| author_sort | Zohaib Khurshid |
| collection | DOAJ |
| description | This study aimed to produce hydroxyapatite from the dentine portion of camel teeth using a defatting and deproteinizing procedure and characterize its physicochemical and biocompatibility properties. Biowaste such as waste camel teeth is a valuable source of hydroxyapatite, the main inorganic constituent of human bone and teeth which is frequently used as bone grafts in the biomedical field. Fourier Transform infrared (FTIR), and micro-Raman spectroscopy confirmed the functional groups as-sociated with hydroxyapatite. X-ray diffraction (XRD) studies showed camel dentine-derived hydroxyapatite (CDHA) corresponded with hydroxyapatite spectra. Scanning electron micros-copy (SEM) demonstrated the presence of dentinal tubules measuring from 1.69–2.91 µm. The inorganic phases of CDHA were primarily constituted of calcium and phosphorus, with trace levels of sodium, magnesium, potassium, and strontium, according to energy dispersive X-ray analysis (EDX) and inductively coupled plasma mass spectrometry (ICP-MS). After 28 days of incubation in simulated body fluid (SBF), the pH of the CDHA scaffold elevated to 9.2. in-vitro biocompatibility studies showed that the CDHA enabled Saos-2 cells to proliferate and express the bone marker osteonectin after 14 days of culture. For applications such as bone augmentation and filling bone gaps, CDHA offers a promising material. However, to evaluate the clinical feasibility of the CDHA, further in-vivo studies are required. |
| first_indexed | 2024-03-09T08:21:42Z |
| format | Article |
| id | doaj.art-6acf3bdb6fef430499270745e47ddebd |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:21:42Z |
| publishDate | 2023-08-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-6acf3bdb6fef430499270745e47ddebd2023-12-02T21:42:54ZengPeerJ Inc.PeerJ2167-83592023-08-0111e1571110.7717/peerj.15711Development, physicochemical characterization and in-vitro biocompatibility study of dromedary camel dentine derived hydroxyapatite for bone repairZohaib Khurshid0Mohammed Farhan A. Alfarhan1Yasmin Bayan2Javed Mazher3Necdet Adanir4George J. Dias5Paul R. Cooper6Jithendra Ratnayake7Department of Prosthodontics and Dental Implantology, College of Dentistry, King Faisal University, Al-Ahsa, Saudi ArabiaDepartment of Surgery, College of Medicine, King Faisal University, Al-Ahsa, Saudi ArabiaDepartment of Oral Science, Faculty of Dentistry, University of Otago, Dunedin, New ZealandDepartment of Physics, College of Science, King Faisal University, Al-Ahsa, Saudi ArabiaDepartment of Restorative Dentistry, College of Dentistry, King Faisal University, Al-Ahsa, Saudi ArabiaDepartment of Anatomy, University of Otago, Dunedin, New ZealandDepartment of Oral Science, Faculty of Dentistry, University of Otago, Dunedin, New ZealandDepartment of Oral Science, Faculty of Dentistry, University of Otago, Dunedin, New ZealandThis study aimed to produce hydroxyapatite from the dentine portion of camel teeth using a defatting and deproteinizing procedure and characterize its physicochemical and biocompatibility properties. Biowaste such as waste camel teeth is a valuable source of hydroxyapatite, the main inorganic constituent of human bone and teeth which is frequently used as bone grafts in the biomedical field. Fourier Transform infrared (FTIR), and micro-Raman spectroscopy confirmed the functional groups as-sociated with hydroxyapatite. X-ray diffraction (XRD) studies showed camel dentine-derived hydroxyapatite (CDHA) corresponded with hydroxyapatite spectra. Scanning electron micros-copy (SEM) demonstrated the presence of dentinal tubules measuring from 1.69–2.91 µm. The inorganic phases of CDHA were primarily constituted of calcium and phosphorus, with trace levels of sodium, magnesium, potassium, and strontium, according to energy dispersive X-ray analysis (EDX) and inductively coupled plasma mass spectrometry (ICP-MS). After 28 days of incubation in simulated body fluid (SBF), the pH of the CDHA scaffold elevated to 9.2. in-vitro biocompatibility studies showed that the CDHA enabled Saos-2 cells to proliferate and express the bone marker osteonectin after 14 days of culture. For applications such as bone augmentation and filling bone gaps, CDHA offers a promising material. However, to evaluate the clinical feasibility of the CDHA, further in-vivo studies are required.https://peerj.com/articles/15711.pdfToothDentineCamelBone cementHydroxyapatiteDental graft |
| spellingShingle | Zohaib Khurshid Mohammed Farhan A. Alfarhan Yasmin Bayan Javed Mazher Necdet Adanir George J. Dias Paul R. Cooper Jithendra Ratnayake Development, physicochemical characterization and in-vitro biocompatibility study of dromedary camel dentine derived hydroxyapatite for bone repair PeerJ Tooth Dentine Camel Bone cement Hydroxyapatite Dental graft |
| title | Development, physicochemical characterization and in-vitro biocompatibility study of dromedary camel dentine derived hydroxyapatite for bone repair |
| title_full | Development, physicochemical characterization and in-vitro biocompatibility study of dromedary camel dentine derived hydroxyapatite for bone repair |
| title_fullStr | Development, physicochemical characterization and in-vitro biocompatibility study of dromedary camel dentine derived hydroxyapatite for bone repair |
| title_full_unstemmed | Development, physicochemical characterization and in-vitro biocompatibility study of dromedary camel dentine derived hydroxyapatite for bone repair |
| title_short | Development, physicochemical characterization and in-vitro biocompatibility study of dromedary camel dentine derived hydroxyapatite for bone repair |
| title_sort | development physicochemical characterization and in vitro biocompatibility study of dromedary camel dentine derived hydroxyapatite for bone repair |
| topic | Tooth Dentine Camel Bone cement Hydroxyapatite Dental graft |
| url | https://peerj.com/articles/15711.pdf |
| work_keys_str_mv | AT zohaibkhurshid developmentphysicochemicalcharacterizationandinvitrobiocompatibilitystudyofdromedarycameldentinederivedhydroxyapatiteforbonerepair AT mohammedfarhanaalfarhan developmentphysicochemicalcharacterizationandinvitrobiocompatibilitystudyofdromedarycameldentinederivedhydroxyapatiteforbonerepair AT yasminbayan developmentphysicochemicalcharacterizationandinvitrobiocompatibilitystudyofdromedarycameldentinederivedhydroxyapatiteforbonerepair AT javedmazher developmentphysicochemicalcharacterizationandinvitrobiocompatibilitystudyofdromedarycameldentinederivedhydroxyapatiteforbonerepair AT necdetadanir developmentphysicochemicalcharacterizationandinvitrobiocompatibilitystudyofdromedarycameldentinederivedhydroxyapatiteforbonerepair AT georgejdias developmentphysicochemicalcharacterizationandinvitrobiocompatibilitystudyofdromedarycameldentinederivedhydroxyapatiteforbonerepair AT paulrcooper developmentphysicochemicalcharacterizationandinvitrobiocompatibilitystudyofdromedarycameldentinederivedhydroxyapatiteforbonerepair AT jithendraratnayake developmentphysicochemicalcharacterizationandinvitrobiocompatibilitystudyofdromedarycameldentinederivedhydroxyapatiteforbonerepair |