Characterization of the AP2/ERF Transcription Factor Family and Expression Profiling of DREB Subfamily under Cold and Osmotic Stresses in <i>Ammopiptanthus nanus</i>
APETALA2/ethylene-responsive factor (AP2/ERF) is one of the largest transcription factor (TF) families in plants, which play important roles in regulating plant growth, development, and response to environmental stresses. <i>Ammopiptanthus nanus</i>, an unusual evergreen broad-leaved shr...
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MDPI AG
2020-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/4/455 |
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| author | Shilin Cao Ying Wang Xuting Li Fei Gao Jinchao Feng Yijun Zhou |
| author_facet | Shilin Cao Ying Wang Xuting Li Fei Gao Jinchao Feng Yijun Zhou |
| author_sort | Shilin Cao |
| collection | DOAJ |
| description | APETALA2/ethylene-responsive factor (AP2/ERF) is one of the largest transcription factor (TF) families in plants, which play important roles in regulating plant growth, development, and response to environmental stresses. <i>Ammopiptanthus nanus</i>, an unusual evergreen broad-leaved shrub in the arid region in the northern temperate zone, demonstrates a strong tolerance to low temperature and drought stresses, and AP2/ERF transcription factors may contribute to the stress tolerance of <i>A. nanus</i>. In the current study, 174 AP2/ERF family members were identified from the <i>A. nanus</i> genome, and they were divided into five subfamilies, including 92 ERF members, 55 dehydration-responsive element binding (DREB) members, 24 AP2 members, 2 RAV members, and 1 Soloist member. Compared with the other leguminous plants, <i>A. nanus</i> has more members of the DREB subfamily and the B1 group of the ERF subfamily, and gene expansion in the AP2/ERF family is primarily driven by tandem and segmental duplications. Promoter analysis showed that many stress-related <i>cis</i>-acting elements existed in promoter regions of the DREB genes, implying that MYB, ICE1, and WRKY transcription factors regulate the expression of DREB genes in <i>A. nanus</i>. Expression profiling revealed that the majority of DREB members were responsive to osmotic and cold stresses, and several DREB genes such as <i>EVM0023336.1</i> and <i>EVM0013392.1</i> were highly induced by cold stress, which may play important roles in cold response in <i>A. nanus</i>. This study provided important data for understanding the evolution and functions of AP2/ERF and DREB transcription factors in <i>A. nanus</i>. |
| first_indexed | 2024-03-10T20:41:02Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-10T20:41:02Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-6acffc4320e0404aa004d2914dacb5c42023-11-19T20:42:04ZengMDPI AGPlants2223-77472020-04-019445510.3390/plants9040455Characterization of the AP2/ERF Transcription Factor Family and Expression Profiling of DREB Subfamily under Cold and Osmotic Stresses in <i>Ammopiptanthus nanus</i>Shilin Cao0Ying Wang1Xuting Li2Fei Gao3Jinchao Feng4Yijun Zhou5College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, ChinaCollege of Life and Environmental Sciences, Minzu University of China, Beijing 100081, ChinaCollege of Life and Environmental Sciences, Minzu University of China, Beijing 100081, ChinaCollege of Life and Environmental Sciences, Minzu University of China, Beijing 100081, ChinaCollege of Life and Environmental Sciences, Minzu University of China, Beijing 100081, ChinaCollege of Life and Environmental Sciences, Minzu University of China, Beijing 100081, ChinaAPETALA2/ethylene-responsive factor (AP2/ERF) is one of the largest transcription factor (TF) families in plants, which play important roles in regulating plant growth, development, and response to environmental stresses. <i>Ammopiptanthus nanus</i>, an unusual evergreen broad-leaved shrub in the arid region in the northern temperate zone, demonstrates a strong tolerance to low temperature and drought stresses, and AP2/ERF transcription factors may contribute to the stress tolerance of <i>A. nanus</i>. In the current study, 174 AP2/ERF family members were identified from the <i>A. nanus</i> genome, and they were divided into five subfamilies, including 92 ERF members, 55 dehydration-responsive element binding (DREB) members, 24 AP2 members, 2 RAV members, and 1 Soloist member. Compared with the other leguminous plants, <i>A. nanus</i> has more members of the DREB subfamily and the B1 group of the ERF subfamily, and gene expansion in the AP2/ERF family is primarily driven by tandem and segmental duplications. Promoter analysis showed that many stress-related <i>cis</i>-acting elements existed in promoter regions of the DREB genes, implying that MYB, ICE1, and WRKY transcription factors regulate the expression of DREB genes in <i>A. nanus</i>. Expression profiling revealed that the majority of DREB members were responsive to osmotic and cold stresses, and several DREB genes such as <i>EVM0023336.1</i> and <i>EVM0013392.1</i> were highly induced by cold stress, which may play important roles in cold response in <i>A. nanus</i>. This study provided important data for understanding the evolution and functions of AP2/ERF and DREB transcription factors in <i>A. nanus</i>.https://www.mdpi.com/2223-7747/9/4/455Ammopiptanthus nanusAP2/ERFtranscription factorgene familyosmotic stresscold stress |
| spellingShingle | Shilin Cao Ying Wang Xuting Li Fei Gao Jinchao Feng Yijun Zhou Characterization of the AP2/ERF Transcription Factor Family and Expression Profiling of DREB Subfamily under Cold and Osmotic Stresses in <i>Ammopiptanthus nanus</i> Plants Ammopiptanthus nanus AP2/ERF transcription factor gene family osmotic stress cold stress |
| title | Characterization of the AP2/ERF Transcription Factor Family and Expression Profiling of DREB Subfamily under Cold and Osmotic Stresses in <i>Ammopiptanthus nanus</i> |
| title_full | Characterization of the AP2/ERF Transcription Factor Family and Expression Profiling of DREB Subfamily under Cold and Osmotic Stresses in <i>Ammopiptanthus nanus</i> |
| title_fullStr | Characterization of the AP2/ERF Transcription Factor Family and Expression Profiling of DREB Subfamily under Cold and Osmotic Stresses in <i>Ammopiptanthus nanus</i> |
| title_full_unstemmed | Characterization of the AP2/ERF Transcription Factor Family and Expression Profiling of DREB Subfamily under Cold and Osmotic Stresses in <i>Ammopiptanthus nanus</i> |
| title_short | Characterization of the AP2/ERF Transcription Factor Family and Expression Profiling of DREB Subfamily under Cold and Osmotic Stresses in <i>Ammopiptanthus nanus</i> |
| title_sort | characterization of the ap2 erf transcription factor family and expression profiling of dreb subfamily under cold and osmotic stresses in i ammopiptanthus nanus i |
| topic | Ammopiptanthus nanus AP2/ERF transcription factor gene family osmotic stress cold stress |
| url | https://www.mdpi.com/2223-7747/9/4/455 |
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