Endophytic Streptomyces sp. NEAU-ZSY13 from the leaf of Perilla frutescens, as a promising broad-spectrum biocontrol agent against soil-borne diseases
Soil-borne diseases cause significant economic losses in global agricultural production. These diseases are challenging to control due to the invasion of multiple pathogens into host plants, and traditional chemical control methods often yield unsatisfactory results. In this study, we isolated and i...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1243610/full |
| _version_ | 1797737583777677312 |
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| author | Zhiyan Wang Zhiyan Wang Congting Gao Congting Gao Jingquan Yang Rui Du Fanli Zeng Hui Bing Banghua Xia Yue Shen Chongxi Liu Chongxi Liu |
| author_facet | Zhiyan Wang Zhiyan Wang Congting Gao Congting Gao Jingquan Yang Rui Du Fanli Zeng Hui Bing Banghua Xia Yue Shen Chongxi Liu Chongxi Liu |
| author_sort | Zhiyan Wang |
| collection | DOAJ |
| description | Soil-borne diseases cause significant economic losses in global agricultural production. These diseases are challenging to control due to the invasion of multiple pathogens into host plants, and traditional chemical control methods often yield unsatisfactory results. In this study, we isolated and identified an endophytic Streptomyces, designated as NEAU-ZSY13, from the leaf of Perilla frutescens. This isolate exhibited broad-spectrum antifungal activity against 17 soil-borne phytopathogenic fungi, with Bipolaris sorokiniana being the most prominent. Additionally, it displayed strong antibacterial activity against the soil-borne phytopathogenic bacterium Ralstonia solanacearum. To assess its biocontrol potential, the isolate was utilized to produce a biofertilizer through solid-state fermentation. The fermentation conditions were optimized using response surface methodology to maximize the spore production. The results revealed that more abundant spores were produced with a 1:2 ratio of vermicompost to wheat bran, 60% water content, 20% inoculation amount and 28°C. Subsequent pot experiments demonstrated that the application of the biofertilizer with a spore concentration of 108 CFU/g soil effectively suppressed the occurrence of tomato bacterial wilt caused by R. solanacearum and wheat root rot caused by B. sorokiniana, with biocontrol efficacies of 72.2 and 78.3%, respectively. Chemical analysis of NEAU-ZSY13 extracts, using nuclear magnetic resonance spectrometry and mass analysis, identified niphimycin C and niphimycin A as the primary active constituents. These compounds exhibited high activity against R. solanacearum (EC50 of 3.6 and 2.4 μg mL−1) and B. sorokiniana (EC50 of 3.9 and 3.4 μg mL−1). In conclusion, this study demonstrates the potential of Streptomyces sp. NEAU-ZSY13 as a biofertilizer for the control of soil-borne diseases. |
| first_indexed | 2024-03-12T13:30:26Z |
| format | Article |
| id | doaj.art-6ad408c5d94e4cce996d56aff3cc6358 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T13:30:26Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-6ad408c5d94e4cce996d56aff3cc63582023-08-24T12:07:19ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-08-011410.3389/fmicb.2023.12436101243610Endophytic Streptomyces sp. NEAU-ZSY13 from the leaf of Perilla frutescens, as a promising broad-spectrum biocontrol agent against soil-borne diseasesZhiyan Wang0Zhiyan Wang1Congting Gao2Congting Gao3Jingquan Yang4Rui Du5Fanli Zeng6Hui Bing7Banghua Xia8Yue Shen9Chongxi Liu10Chongxi Liu11Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin, ChinaDepartment of Molecular Pharmacology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, ChinaKey Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin, ChinaHebei Technology Innovation Center for Green Management of Soil-borne Diseases, Baoding University, Baoding, ChinaKey Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin, ChinaKey Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin, ChinaHebei Technology Innovation Center for Green Management of Soil-borne Diseases, Baoding University, Baoding, ChinaSoil-borne diseases cause significant economic losses in global agricultural production. These diseases are challenging to control due to the invasion of multiple pathogens into host plants, and traditional chemical control methods often yield unsatisfactory results. In this study, we isolated and identified an endophytic Streptomyces, designated as NEAU-ZSY13, from the leaf of Perilla frutescens. This isolate exhibited broad-spectrum antifungal activity against 17 soil-borne phytopathogenic fungi, with Bipolaris sorokiniana being the most prominent. Additionally, it displayed strong antibacterial activity against the soil-borne phytopathogenic bacterium Ralstonia solanacearum. To assess its biocontrol potential, the isolate was utilized to produce a biofertilizer through solid-state fermentation. The fermentation conditions were optimized using response surface methodology to maximize the spore production. The results revealed that more abundant spores were produced with a 1:2 ratio of vermicompost to wheat bran, 60% water content, 20% inoculation amount and 28°C. Subsequent pot experiments demonstrated that the application of the biofertilizer with a spore concentration of 108 CFU/g soil effectively suppressed the occurrence of tomato bacterial wilt caused by R. solanacearum and wheat root rot caused by B. sorokiniana, with biocontrol efficacies of 72.2 and 78.3%, respectively. Chemical analysis of NEAU-ZSY13 extracts, using nuclear magnetic resonance spectrometry and mass analysis, identified niphimycin C and niphimycin A as the primary active constituents. These compounds exhibited high activity against R. solanacearum (EC50 of 3.6 and 2.4 μg mL−1) and B. sorokiniana (EC50 of 3.9 and 3.4 μg mL−1). In conclusion, this study demonstrates the potential of Streptomyces sp. NEAU-ZSY13 as a biofertilizer for the control of soil-borne diseases.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1243610/fullsoil-borne diseasesendophytic actinobacteriabroad-spectrum antimicrobial activitybiocontrolniphimycin |
| spellingShingle | Zhiyan Wang Zhiyan Wang Congting Gao Congting Gao Jingquan Yang Rui Du Fanli Zeng Hui Bing Banghua Xia Yue Shen Chongxi Liu Chongxi Liu Endophytic Streptomyces sp. NEAU-ZSY13 from the leaf of Perilla frutescens, as a promising broad-spectrum biocontrol agent against soil-borne diseases Frontiers in Microbiology soil-borne diseases endophytic actinobacteria broad-spectrum antimicrobial activity biocontrol niphimycin |
| title | Endophytic Streptomyces sp. NEAU-ZSY13 from the leaf of Perilla frutescens, as a promising broad-spectrum biocontrol agent against soil-borne diseases |
| title_full | Endophytic Streptomyces sp. NEAU-ZSY13 from the leaf of Perilla frutescens, as a promising broad-spectrum biocontrol agent against soil-borne diseases |
| title_fullStr | Endophytic Streptomyces sp. NEAU-ZSY13 from the leaf of Perilla frutescens, as a promising broad-spectrum biocontrol agent against soil-borne diseases |
| title_full_unstemmed | Endophytic Streptomyces sp. NEAU-ZSY13 from the leaf of Perilla frutescens, as a promising broad-spectrum biocontrol agent against soil-borne diseases |
| title_short | Endophytic Streptomyces sp. NEAU-ZSY13 from the leaf of Perilla frutescens, as a promising broad-spectrum biocontrol agent against soil-borne diseases |
| title_sort | endophytic streptomyces sp neau zsy13 from the leaf of perilla frutescens as a promising broad spectrum biocontrol agent against soil borne diseases |
| topic | soil-borne diseases endophytic actinobacteria broad-spectrum antimicrobial activity biocontrol niphimycin |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1243610/full |
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