Near-infrared-responsive silver-capped magnetic nanoclusters for cancer therapy
Aim: Near-infrared (NIR)-based photothermal therapy (PTT) has been proposed as a prospective adjuvant to traditional chemotherapy. The present work aims to study the impact of silver-coated magnetic nanoparticles as a PTT agent against multiple cancer cell lines. Materials and Methods: Silver-coated...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2020-01-01
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| Series: | Journal of Radiation and Cancer Research |
| Subjects: | |
| Online Access: | http://www.journalrcr.org/article.asp?issn=2588-9273;year=2020;volume=11;issue=2;spage=45;epage=51;aulast=Tewari |
| _version_ | 1818139422215897088 |
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| author | Amit Tewari Ruby Gupta Deepika Sharma |
| author_facet | Amit Tewari Ruby Gupta Deepika Sharma |
| author_sort | Amit Tewari |
| collection | DOAJ |
| description | Aim: Near-infrared (NIR)-based photothermal therapy (PTT) has been proposed as a prospective adjuvant to traditional chemotherapy. The present work aims to study the impact of silver-coated magnetic nanoparticles as a PTT agent against multiple cancer cell lines. Materials and Methods: Silver-coated magnetic nanoclusters (Ag-MNCs) were synthesized by a modified method and characterized using X-ray diffraction, transmission electron microscopy, Fourier-transform infrared spectroscopy, and ultraviolet–visible absorption spectra. Its effect as an agent for NIR-based PTT was assessed on four different human cell lines, namely glioblastoma cell line U-87 MG, osteosarcoma MG-63, lung carcinoma A549, and triple-negative breast cancer cell line MDA-MB-231 by irradiation with 750 nm NIR laser for 10 min. Cellular damage was assessed in terms of MTT and cell cycle analysis and visualized by confocal microscopy. Results: The Ag-MNCs were successfully generated and exhibited excellent hyperthermic rise when exposed to NIR laser. A reduction of more than 60% of the cells was observed in the MTT assay. Confocal microscopy also confirmed significant nuclear damage to cells exposed to PTT in the presence of Ag-MNCs. Conclusion: Our results confirm that the Ag-MNCs have an excellent hyperthermic profile and as the test results indicate that it can be utilized as an agent for NIR-based PTT against various types of cancer cells. |
| first_indexed | 2024-12-11T10:27:50Z |
| format | Article |
| id | doaj.art-6ad43a4b655240169160bd668bb057d3 |
| institution | Directory Open Access Journal |
| issn | 2588-9273 2468-9203 |
| language | English |
| last_indexed | 2024-12-11T10:27:50Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Radiation and Cancer Research |
| spelling | doaj.art-6ad43a4b655240169160bd668bb057d32022-12-22T01:11:05ZengWolters Kluwer Medknow PublicationsJournal of Radiation and Cancer Research2588-92732468-92032020-01-01112455110.4103/jrcr.jrcr_19_20Near-infrared-responsive silver-capped magnetic nanoclusters for cancer therapyAmit TewariRuby GuptaDeepika SharmaAim: Near-infrared (NIR)-based photothermal therapy (PTT) has been proposed as a prospective adjuvant to traditional chemotherapy. The present work aims to study the impact of silver-coated magnetic nanoparticles as a PTT agent against multiple cancer cell lines. Materials and Methods: Silver-coated magnetic nanoclusters (Ag-MNCs) were synthesized by a modified method and characterized using X-ray diffraction, transmission electron microscopy, Fourier-transform infrared spectroscopy, and ultraviolet–visible absorption spectra. Its effect as an agent for NIR-based PTT was assessed on four different human cell lines, namely glioblastoma cell line U-87 MG, osteosarcoma MG-63, lung carcinoma A549, and triple-negative breast cancer cell line MDA-MB-231 by irradiation with 750 nm NIR laser for 10 min. Cellular damage was assessed in terms of MTT and cell cycle analysis and visualized by confocal microscopy. Results: The Ag-MNCs were successfully generated and exhibited excellent hyperthermic rise when exposed to NIR laser. A reduction of more than 60% of the cells was observed in the MTT assay. Confocal microscopy also confirmed significant nuclear damage to cells exposed to PTT in the presence of Ag-MNCs. Conclusion: Our results confirm that the Ag-MNCs have an excellent hyperthermic profile and as the test results indicate that it can be utilized as an agent for NIR-based PTT against various types of cancer cells.http://www.journalrcr.org/article.asp?issn=2588-9273;year=2020;volume=11;issue=2;spage=45;epage=51;aulast=Tewari human cell linesmagnetic nanoclustersnear infraredphotothermal therapysilver |
| spellingShingle | Amit Tewari Ruby Gupta Deepika Sharma Near-infrared-responsive silver-capped magnetic nanoclusters for cancer therapy Journal of Radiation and Cancer Research human cell lines magnetic nanoclusters near infrared photothermal therapy silver |
| title | Near-infrared-responsive silver-capped magnetic nanoclusters for cancer therapy |
| title_full | Near-infrared-responsive silver-capped magnetic nanoclusters for cancer therapy |
| title_fullStr | Near-infrared-responsive silver-capped magnetic nanoclusters for cancer therapy |
| title_full_unstemmed | Near-infrared-responsive silver-capped magnetic nanoclusters for cancer therapy |
| title_short | Near-infrared-responsive silver-capped magnetic nanoclusters for cancer therapy |
| title_sort | near infrared responsive silver capped magnetic nanoclusters for cancer therapy |
| topic | human cell lines magnetic nanoclusters near infrared photothermal therapy silver |
| url | http://www.journalrcr.org/article.asp?issn=2588-9273;year=2020;volume=11;issue=2;spage=45;epage=51;aulast=Tewari |
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