A 6-step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model
Introduction: Organotypic Hippocampal Brain Slices (OHBS) provide a better alternative to in vivo models to scrutinize Traumatic Brain Injury (TBI). We followed a well-established TBI protocol but noticed that several factors might influence the results in such a set-up. Here, we describe a structur...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Iran University of Medical Sciences
2019-09-01
|
| Series: | Basic and Clinical Neuroscience |
| Subjects: | |
| Online Access: | http://bcn.iums.ac.ir/article-1-1019-en.html |
| _version_ | 1797280647132217344 |
|---|---|
| author | Linda Grüßer Rosmarie Blaumeiser-Debarry Rolf Rossaint Matthias Krings Benedikt Kremer Anke Höllig Mark Coburn |
| author_facet | Linda Grüßer Rosmarie Blaumeiser-Debarry Rolf Rossaint Matthias Krings Benedikt Kremer Anke Höllig Mark Coburn |
| author_sort | Linda Grüßer |
| collection | DOAJ |
| description | Introduction: Organotypic Hippocampal Brain Slices (OHBS) provide a better alternative to in vivo models to scrutinize Traumatic Brain Injury (TBI). We followed a well-established TBI protocol but noticed that several factors might influence the results in such a set-up. Here, we describe a structured approach to generate more comparable results and discuss why specific eligibility criteria should be applied.
Methods: We defined necessary checkpoints and developed inclusion and exclusion criteria that took into consideration the observed variation in such a model. Objective measures include the identification and exclusion of pre-damaged slices and outliers. In this study, six steps were outlined.
Results: A six-step approach to enhance comparability is proposed and summarized in a flowchart. We applied the suggested measures to data derived from our TBI-experiments, examining the impact of three different interventions in 1459 OHBS. Our exemplary results show that more precise findings are ensured through equal requirements set for all slices.
Conclusion: Results in a TBI experiment on OHBS should be critically analyzed as inhomogeneity may occur. In other words, a structured approach to comparing the results should be followed to ensure achieving more precise findings. Further research is recommended to confirm and further develop this framework. |
| first_indexed | 2024-03-07T16:43:17Z |
| format | Article |
| id | doaj.art-6ad701086da34040bd55a496e0baa51b |
| institution | Directory Open Access Journal |
| issn | 2008-126X 2228-7442 |
| language | English |
| last_indexed | 2024-03-07T16:43:17Z |
| publishDate | 2019-09-01 |
| publisher | Iran University of Medical Sciences |
| record_format | Article |
| series | Basic and Clinical Neuroscience |
| spelling | doaj.art-6ad701086da34040bd55a496e0baa51b2024-03-03T07:15:18ZengIran University of Medical SciencesBasic and Clinical Neuroscience2008-126X2228-74422019-09-01105485496A 6-step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury ModelLinda Grüßer0Rosmarie Blaumeiser-Debarry1Rolf Rossaint2Matthias Krings3Benedikt Kremer4Anke Höllig5Mark Coburn6 Department of Anesthesiology, RWTH Aachen University Hospital, Aachen, Germany. Department of Anesthesiology, RWTH Aachen University Hospital, Aachen, Germany. Department of Anesthesiology, RWTH Aachen University Hospital, Aachen, Germany. Department of Anesthesiology, RWTH Aachen University Hospital, Aachen, Germany. Department of Neurosurgery, RWTH Aachen University Hospital, Pauwelsstr. 30, 52074 Aachen, Germany Department of Neurosurgery, RWTH Aachen University Hospital, Aachen, Germany. Department of Anesthesiology, RWTH Aachen University Hospital, Aachen, Germany. Introduction: Organotypic Hippocampal Brain Slices (OHBS) provide a better alternative to in vivo models to scrutinize Traumatic Brain Injury (TBI). We followed a well-established TBI protocol but noticed that several factors might influence the results in such a set-up. Here, we describe a structured approach to generate more comparable results and discuss why specific eligibility criteria should be applied. Methods: We defined necessary checkpoints and developed inclusion and exclusion criteria that took into consideration the observed variation in such a model. Objective measures include the identification and exclusion of pre-damaged slices and outliers. In this study, six steps were outlined. Results: A six-step approach to enhance comparability is proposed and summarized in a flowchart. We applied the suggested measures to data derived from our TBI-experiments, examining the impact of three different interventions in 1459 OHBS. Our exemplary results show that more precise findings are ensured through equal requirements set for all slices. Conclusion: Results in a TBI experiment on OHBS should be critically analyzed as inhomogeneity may occur. In other words, a structured approach to comparing the results should be followed to ensure achieving more precise findings. Further research is recommended to confirm and further develop this framework.http://bcn.iums.ac.ir/article-1-1019-en.htmlorganotypic hippocampal brain slicesin vitro modeltraumatic brain injury (tbi)propidium iodide (pi)eligibilityframework |
| spellingShingle | Linda Grüßer Rosmarie Blaumeiser-Debarry Rolf Rossaint Matthias Krings Benedikt Kremer Anke Höllig Mark Coburn A 6-step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model Basic and Clinical Neuroscience organotypic hippocampal brain slices in vitro model traumatic brain injury (tbi) propidium iodide (pi) eligibility framework |
| title | A 6-step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model |
| title_full | A 6-step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model |
| title_fullStr | A 6-step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model |
| title_full_unstemmed | A 6-step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model |
| title_short | A 6-step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model |
| title_sort | 6 step approach to gain higher quality results from organotypic hippocampal brain slices in a traumatic brain injury model |
| topic | organotypic hippocampal brain slices in vitro model traumatic brain injury (tbi) propidium iodide (pi) eligibility framework |
| url | http://bcn.iums.ac.ir/article-1-1019-en.html |
| work_keys_str_mv | AT lindagrußer a6stepapproachtogainhigherqualityresultsfromorganotypichippocampalbrainslicesinatraumaticbraininjurymodel AT rosmarieblaumeiserdebarry a6stepapproachtogainhigherqualityresultsfromorganotypichippocampalbrainslicesinatraumaticbraininjurymodel AT rolfrossaint a6stepapproachtogainhigherqualityresultsfromorganotypichippocampalbrainslicesinatraumaticbraininjurymodel AT matthiaskrings a6stepapproachtogainhigherqualityresultsfromorganotypichippocampalbrainslicesinatraumaticbraininjurymodel AT benediktkremer a6stepapproachtogainhigherqualityresultsfromorganotypichippocampalbrainslicesinatraumaticbraininjurymodel AT ankehollig a6stepapproachtogainhigherqualityresultsfromorganotypichippocampalbrainslicesinatraumaticbraininjurymodel AT markcoburn a6stepapproachtogainhigherqualityresultsfromorganotypichippocampalbrainslicesinatraumaticbraininjurymodel AT lindagrußer 6stepapproachtogainhigherqualityresultsfromorganotypichippocampalbrainslicesinatraumaticbraininjurymodel AT rosmarieblaumeiserdebarry 6stepapproachtogainhigherqualityresultsfromorganotypichippocampalbrainslicesinatraumaticbraininjurymodel AT rolfrossaint 6stepapproachtogainhigherqualityresultsfromorganotypichippocampalbrainslicesinatraumaticbraininjurymodel AT matthiaskrings 6stepapproachtogainhigherqualityresultsfromorganotypichippocampalbrainslicesinatraumaticbraininjurymodel AT benediktkremer 6stepapproachtogainhigherqualityresultsfromorganotypichippocampalbrainslicesinatraumaticbraininjurymodel AT ankehollig 6stepapproachtogainhigherqualityresultsfromorganotypichippocampalbrainslicesinatraumaticbraininjurymodel AT markcoburn 6stepapproachtogainhigherqualityresultsfromorganotypichippocampalbrainslicesinatraumaticbraininjurymodel |