Isaria cicadae Miquel prevents intestinal fibrosis by activating transforming growth factor-β1 signaling to regulate the balance between matrix metalloproteinases and tissue inhibitors of metalloproteinase 1 in mice with Crohn’s disease
We aim at exploring the potential therapeutic effect and mechanisms of artificial Isaria cicadae Miquel (IcM) in Crohn’s disease (CD)-related intestinal fibrosis. The Crohn's fibrosis model was induced by Trinitrobenzene sulphonic acid (TNBS). Mice were divided into control group, TNBS control...
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Elsevier
2022-01-01
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Series: | Journal of Functional Foods |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464621005247 |
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author | Hui Wang Zhe Yang Kang Chen Ruilian Yu Li Xu Gaohong Lv |
author_facet | Hui Wang Zhe Yang Kang Chen Ruilian Yu Li Xu Gaohong Lv |
author_sort | Hui Wang |
collection | DOAJ |
description | We aim at exploring the potential therapeutic effect and mechanisms of artificial Isaria cicadae Miquel (IcM) in Crohn’s disease (CD)-related intestinal fibrosis. The Crohn's fibrosis model was induced by Trinitrobenzene sulphonic acid (TNBS). Mice were divided into control group, TNBS control group, and IcM treatment group (H, M, D). After four weeks, the entire length of the colon and serum were collected, and Hematoxylin and Eosin staining and Masson’s collagen trichrome staining were performed for determining the colon histological score. The contents of TGF-β1, CTGF, and IFN-γ were determined by ELISA. FN and α-SAM were evaluated by Western blotting; further, MMP-1, MMP-3, and TIMP-1 mRNA were determined by qRT-PCR. We observed that IcM prevents intestinal fibrosis in mice with CD. In addition, IcM was capable of significantly down-regulating the expression of major fibrosis markers (⍺-SMA and fiber-fibroin) and key fibrogenic molecules TGF-β1 and CTGF, and promoting the expression of IFN-γ, which has antifibrotic effects to maintain MMP/TIMP balance. |
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institution | Directory Open Access Journal |
issn | 1756-4646 |
language | English |
last_indexed | 2024-12-17T23:43:06Z |
publishDate | 2022-01-01 |
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series | Journal of Functional Foods |
spelling | doaj.art-6ad78384075c472a80678c9e77cad0ef2022-12-21T21:28:22ZengElsevierJournal of Functional Foods1756-46462022-01-0188104875Isaria cicadae Miquel prevents intestinal fibrosis by activating transforming growth factor-β1 signaling to regulate the balance between matrix metalloproteinases and tissue inhibitors of metalloproteinase 1 in mice with Crohn’s diseaseHui Wang0Zhe Yang1Kang Chen2Ruilian Yu3Li Xu4Gaohong Lv5School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCorresponding author.; School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaWe aim at exploring the potential therapeutic effect and mechanisms of artificial Isaria cicadae Miquel (IcM) in Crohn’s disease (CD)-related intestinal fibrosis. The Crohn's fibrosis model was induced by Trinitrobenzene sulphonic acid (TNBS). Mice were divided into control group, TNBS control group, and IcM treatment group (H, M, D). After four weeks, the entire length of the colon and serum were collected, and Hematoxylin and Eosin staining and Masson’s collagen trichrome staining were performed for determining the colon histological score. The contents of TGF-β1, CTGF, and IFN-γ were determined by ELISA. FN and α-SAM were evaluated by Western blotting; further, MMP-1, MMP-3, and TIMP-1 mRNA were determined by qRT-PCR. We observed that IcM prevents intestinal fibrosis in mice with CD. In addition, IcM was capable of significantly down-regulating the expression of major fibrosis markers (⍺-SMA and fiber-fibroin) and key fibrogenic molecules TGF-β1 and CTGF, and promoting the expression of IFN-γ, which has antifibrotic effects to maintain MMP/TIMP balance.http://www.sciencedirect.com/science/article/pii/S1756464621005247IcMTNBSCrohn's diseaseIntestinal fibrosisTransforming growth factor-β1Extracellular matrix |
spellingShingle | Hui Wang Zhe Yang Kang Chen Ruilian Yu Li Xu Gaohong Lv Isaria cicadae Miquel prevents intestinal fibrosis by activating transforming growth factor-β1 signaling to regulate the balance between matrix metalloproteinases and tissue inhibitors of metalloproteinase 1 in mice with Crohn’s disease Journal of Functional Foods IcM TNBS Crohn's disease Intestinal fibrosis Transforming growth factor-β1 Extracellular matrix |
title | Isaria cicadae Miquel prevents intestinal fibrosis by activating transforming growth factor-β1 signaling to regulate the balance between matrix metalloproteinases and tissue inhibitors of metalloproteinase 1 in mice with Crohn’s disease |
title_full | Isaria cicadae Miquel prevents intestinal fibrosis by activating transforming growth factor-β1 signaling to regulate the balance between matrix metalloproteinases and tissue inhibitors of metalloproteinase 1 in mice with Crohn’s disease |
title_fullStr | Isaria cicadae Miquel prevents intestinal fibrosis by activating transforming growth factor-β1 signaling to regulate the balance between matrix metalloproteinases and tissue inhibitors of metalloproteinase 1 in mice with Crohn’s disease |
title_full_unstemmed | Isaria cicadae Miquel prevents intestinal fibrosis by activating transforming growth factor-β1 signaling to regulate the balance between matrix metalloproteinases and tissue inhibitors of metalloproteinase 1 in mice with Crohn’s disease |
title_short | Isaria cicadae Miquel prevents intestinal fibrosis by activating transforming growth factor-β1 signaling to regulate the balance between matrix metalloproteinases and tissue inhibitors of metalloproteinase 1 in mice with Crohn’s disease |
title_sort | isaria cicadae miquel prevents intestinal fibrosis by activating transforming growth factor β1 signaling to regulate the balance between matrix metalloproteinases and tissue inhibitors of metalloproteinase 1 in mice with crohn s disease |
topic | IcM TNBS Crohn's disease Intestinal fibrosis Transforming growth factor-β1 Extracellular matrix |
url | http://www.sciencedirect.com/science/article/pii/S1756464621005247 |
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