Melatonin Supplementation Decreases Hypertrophic Obesity and Inflammation Induced by High-Fat Diet in Mice
Obesity results from critical periods of positive energy balance characterized by caloric intake greater than energy expenditure. This disbalance promotes adipose tissue dysfunction which is related to other comorbidities. Melatonin is a low-cost therapeutic agent and studies indicate that its use m...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-11-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fendo.2019.00750/full |
| _version_ | 1818321732065296384 |
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| author | Talita da Silva Mendes de Farias Maysa Mariana Cruz Roberta Cavalcante da Cunha de Sa Ilenia Severi Ilenia Severi Jessica Perugini Jessica Perugini Martina Senzacqua Martina Senzacqua Suzete Maria Cerutti Suzete Maria Cerutti Antonio Giordano Antonio Giordano Saverio Cinti Saverio Cinti Maria Isabel Cardoso Alonso-Vale Maria Isabel Cardoso Alonso-Vale |
| author_facet | Talita da Silva Mendes de Farias Maysa Mariana Cruz Roberta Cavalcante da Cunha de Sa Ilenia Severi Ilenia Severi Jessica Perugini Jessica Perugini Martina Senzacqua Martina Senzacqua Suzete Maria Cerutti Suzete Maria Cerutti Antonio Giordano Antonio Giordano Saverio Cinti Saverio Cinti Maria Isabel Cardoso Alonso-Vale Maria Isabel Cardoso Alonso-Vale |
| author_sort | Talita da Silva Mendes de Farias |
| collection | DOAJ |
| description | Obesity results from critical periods of positive energy balance characterized by caloric intake greater than energy expenditure. This disbalance promotes adipose tissue dysfunction which is related to other comorbidities. Melatonin is a low-cost therapeutic agent and studies indicate that its use may improve obesity-related disorders. To evaluate if the melatonin is efficient in delaying or even blocking the damages caused by excessive ingestion of a high-fat diet (HFD) in mice, as well as improving the inflammatory profile triggered by obesity herein, male C57BL/6 mice of 8 weeks were induced to obesity by a HFD and treated for 10 weeks with melatonin. The results demonstrate that melatonin supplementation attenuated serum triglyceride levels and total and LDL cholesterol and prevented body mass gain through a decreased lipogenesis rate and increased lipolytic capacity in white adipocytes, with a concomitant increment in oxygen consumption and Pgc1a and Prdm16 expression. Altogether, these effects prevented adipocyte hypertrophy caused by HFD and reflected in decreased adiposity. Finally, melatonin supplementation reduced the crown-like-structure (CLS) formation, characteristic of the inflammatory process by macrophage infiltration into white adipose tissue of obese subjects, as well as decreased the gene expression of inflammation-related factors, such as leptin and MCP1. Thus, the melatonin can be considered a potential therapeutic agent to attenuate the metabolic and inflammatory disorders triggered by obesity. |
| first_indexed | 2024-12-13T10:45:34Z |
| format | Article |
| id | doaj.art-6ad90cdd28ba4fc89b4d24d9e9257096 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-13T10:45:34Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-6ad90cdd28ba4fc89b4d24d9e92570962022-12-21T23:50:13ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922019-11-011010.3389/fendo.2019.00750494332Melatonin Supplementation Decreases Hypertrophic Obesity and Inflammation Induced by High-Fat Diet in MiceTalita da Silva Mendes de Farias0Maysa Mariana Cruz1Roberta Cavalcante da Cunha de Sa2Ilenia Severi3Ilenia Severi4Jessica Perugini5Jessica Perugini6Martina Senzacqua7Martina Senzacqua8Suzete Maria Cerutti9Suzete Maria Cerutti10Antonio Giordano11Antonio Giordano12Saverio Cinti13Saverio Cinti14Maria Isabel Cardoso Alonso-Vale15Maria Isabel Cardoso Alonso-Vale16Post-graduate Program in Chemical Biology, Institute of Environmental Sciences, Chemical and Pharmaceutical, Universidade Federal de São Paulo-UNIFESP, Diadema, BrazilPost-graduate Program in Chemical Biology, Institute of Environmental Sciences, Chemical and Pharmaceutical, Universidade Federal de São Paulo-UNIFESP, Diadema, BrazilPost-graduate Program in Chemical Biology, Institute of Environmental Sciences, Chemical and Pharmaceutical, Universidade Federal de São Paulo-UNIFESP, Diadema, BrazilDepartment of Experimental and Clinical Medicine, University of Ancona (Politecnica Delle Marche), Ancona, ItalyCenter of Obesity, University of Ancona (Politecnica Delle Marche), Ancona, ItalyDepartment of Experimental and Clinical Medicine, University of Ancona (Politecnica Delle Marche), Ancona, ItalyCenter of Obesity, University of Ancona (Politecnica Delle Marche), Ancona, ItalyDepartment of Experimental and Clinical Medicine, University of Ancona (Politecnica Delle Marche), Ancona, ItalyCenter of Obesity, University of Ancona (Politecnica Delle Marche), Ancona, ItalyPost-graduate Program in Chemical Biology, Institute of Environmental Sciences, Chemical and Pharmaceutical, Universidade Federal de São Paulo-UNIFESP, Diadema, BrazilDepartment of Biological Sciences, Institute of Environmental Sciences, Chemical and Pharmaceutical, Universidade Federal de São Paulo-UNIFESP, Diadema, BrazilDepartment of Experimental and Clinical Medicine, University of Ancona (Politecnica Delle Marche), Ancona, ItalyCenter of Obesity, University of Ancona (Politecnica Delle Marche), Ancona, ItalyDepartment of Experimental and Clinical Medicine, University of Ancona (Politecnica Delle Marche), Ancona, ItalyCenter of Obesity, University of Ancona (Politecnica Delle Marche), Ancona, ItalyPost-graduate Program in Chemical Biology, Institute of Environmental Sciences, Chemical and Pharmaceutical, Universidade Federal de São Paulo-UNIFESP, Diadema, BrazilDepartment of Biological Sciences, Institute of Environmental Sciences, Chemical and Pharmaceutical, Universidade Federal de São Paulo-UNIFESP, Diadema, BrazilObesity results from critical periods of positive energy balance characterized by caloric intake greater than energy expenditure. This disbalance promotes adipose tissue dysfunction which is related to other comorbidities. Melatonin is a low-cost therapeutic agent and studies indicate that its use may improve obesity-related disorders. To evaluate if the melatonin is efficient in delaying or even blocking the damages caused by excessive ingestion of a high-fat diet (HFD) in mice, as well as improving the inflammatory profile triggered by obesity herein, male C57BL/6 mice of 8 weeks were induced to obesity by a HFD and treated for 10 weeks with melatonin. The results demonstrate that melatonin supplementation attenuated serum triglyceride levels and total and LDL cholesterol and prevented body mass gain through a decreased lipogenesis rate and increased lipolytic capacity in white adipocytes, with a concomitant increment in oxygen consumption and Pgc1a and Prdm16 expression. Altogether, these effects prevented adipocyte hypertrophy caused by HFD and reflected in decreased adiposity. Finally, melatonin supplementation reduced the crown-like-structure (CLS) formation, characteristic of the inflammatory process by macrophage infiltration into white adipose tissue of obese subjects, as well as decreased the gene expression of inflammation-related factors, such as leptin and MCP1. Thus, the melatonin can be considered a potential therapeutic agent to attenuate the metabolic and inflammatory disorders triggered by obesity.https://www.frontiersin.org/article/10.3389/fendo.2019.00750/fullsubcutaneous fatcytokinesinflammationtriacylglycerolcholesterolbody weight reduction |
| spellingShingle | Talita da Silva Mendes de Farias Maysa Mariana Cruz Roberta Cavalcante da Cunha de Sa Ilenia Severi Ilenia Severi Jessica Perugini Jessica Perugini Martina Senzacqua Martina Senzacqua Suzete Maria Cerutti Suzete Maria Cerutti Antonio Giordano Antonio Giordano Saverio Cinti Saverio Cinti Maria Isabel Cardoso Alonso-Vale Maria Isabel Cardoso Alonso-Vale Melatonin Supplementation Decreases Hypertrophic Obesity and Inflammation Induced by High-Fat Diet in Mice Frontiers in Endocrinology subcutaneous fat cytokines inflammation triacylglycerol cholesterol body weight reduction |
| title | Melatonin Supplementation Decreases Hypertrophic Obesity and Inflammation Induced by High-Fat Diet in Mice |
| title_full | Melatonin Supplementation Decreases Hypertrophic Obesity and Inflammation Induced by High-Fat Diet in Mice |
| title_fullStr | Melatonin Supplementation Decreases Hypertrophic Obesity and Inflammation Induced by High-Fat Diet in Mice |
| title_full_unstemmed | Melatonin Supplementation Decreases Hypertrophic Obesity and Inflammation Induced by High-Fat Diet in Mice |
| title_short | Melatonin Supplementation Decreases Hypertrophic Obesity and Inflammation Induced by High-Fat Diet in Mice |
| title_sort | melatonin supplementation decreases hypertrophic obesity and inflammation induced by high fat diet in mice |
| topic | subcutaneous fat cytokines inflammation triacylglycerol cholesterol body weight reduction |
| url | https://www.frontiersin.org/article/10.3389/fendo.2019.00750/full |
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