Bioactivities of Mealworm (<i>Alphitobius diaperinus</i> L.) Larvae Hydrolysates Obtained from Artichoke (<i>Cynara scolymus</i> L.) Proteases
In this study, we aimed to obtain hydrolysates with bioactive peptides from mealworm (<i>Alphitobius diaperinus</i> L.) larvae using an artichoke (<i>Cynara scolymus</i> L.) enzyme extract. Two types of substrates were used: the raw larvae flour (LF) and its protein extract (...
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MDPI AG
2022-04-01
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| Online Access: | https://www.mdpi.com/2079-7737/11/5/631 |
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| author | Luis Tejada Laura Buendía-Moreno Irene Hernández Adela Abellán José María Cayuela Eva Salazar Estefanía Bueno-Gavilá |
| author_facet | Luis Tejada Laura Buendía-Moreno Irene Hernández Adela Abellán José María Cayuela Eva Salazar Estefanía Bueno-Gavilá |
| author_sort | Luis Tejada |
| collection | DOAJ |
| description | In this study, we aimed to obtain hydrolysates with bioactive peptides from mealworm (<i>Alphitobius diaperinus</i> L.) larvae using an artichoke (<i>Cynara scolymus</i> L.) enzyme extract. Two types of substrates were used: the raw larvae flour (LF) and its protein extract (PE). The hydrolysis yield, considering the peptide concentration of the hydrolysates, was higher in PE hydrolysates than in LF hydrolysates (6.39 ± 0.59 vs. 3.02 ± 0.06 mg/mL, respectively). However, LF showed a higher antioxidant activity against the DPPH radical than PE (59.10 ± 1.42 vs. 18.79 ± 0.81 µM Trolox Eq/mg peptides, respectively). Regarding the inhibitory activity of angiotensin-I-converting enzyme (ACE), an IC<sub>50</sub> value of 111.33 ± 21.3 µg peptides/mL was observed in the PE. The identification of the peptide sequence of both hydrolysates was conducted, and LF and its PE presented 404 and 116 peptides, respectively, most with low molecular weight (<3 kDa), high percentage of hydrophobic amino acids, and typical characteristics of well-known antioxidant and ACE-inhibitory peptides. Furthermore, the potential bioactivity of the sequences identified was searched in the BIOPEP database. Considering the antioxidant and ACE-inhibitory activities, LF hydrolysates contained a larger number of sequences with potential bioactivity than PE hydrolysates. |
| first_indexed | 2024-03-10T03:19:27Z |
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| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T03:19:27Z |
| publishDate | 2022-04-01 |
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| spelling | doaj.art-6ad9a10b34274a29b1d0a8dad6cf67fe2023-11-23T10:06:13ZengMDPI AGBiology2079-77372022-04-0111563110.3390/biology11050631Bioactivities of Mealworm (<i>Alphitobius diaperinus</i> L.) Larvae Hydrolysates Obtained from Artichoke (<i>Cynara scolymus</i> L.) ProteasesLuis Tejada0Laura Buendía-Moreno1Irene Hernández2Adela Abellán3José María Cayuela4Eva Salazar5Estefanía Bueno-Gavilá6Department of Human Nutrition and Food Technology, Universidad Católica de Murcia UCAM Campus de los Jerónimos, 30107 Murcia, SpainDepartment of Human Nutrition and Food Technology, Universidad Católica de Murcia UCAM Campus de los Jerónimos, 30107 Murcia, SpainDepartment of Human Nutrition and Food Technology, Universidad Católica de Murcia UCAM Campus de los Jerónimos, 30107 Murcia, SpainDepartment of Human Nutrition and Food Technology, Universidad Católica de Murcia UCAM Campus de los Jerónimos, 30107 Murcia, SpainDepartment of Human Nutrition and Food Technology, Universidad Católica de Murcia UCAM Campus de los Jerónimos, 30107 Murcia, SpainDepartment of Human Nutrition and Food Technology, Universidad Católica de Murcia UCAM Campus de los Jerónimos, 30107 Murcia, SpainDepartment of Human Nutrition and Food Technology, Universidad Católica de Murcia UCAM Campus de los Jerónimos, 30107 Murcia, SpainIn this study, we aimed to obtain hydrolysates with bioactive peptides from mealworm (<i>Alphitobius diaperinus</i> L.) larvae using an artichoke (<i>Cynara scolymus</i> L.) enzyme extract. Two types of substrates were used: the raw larvae flour (LF) and its protein extract (PE). The hydrolysis yield, considering the peptide concentration of the hydrolysates, was higher in PE hydrolysates than in LF hydrolysates (6.39 ± 0.59 vs. 3.02 ± 0.06 mg/mL, respectively). However, LF showed a higher antioxidant activity against the DPPH radical than PE (59.10 ± 1.42 vs. 18.79 ± 0.81 µM Trolox Eq/mg peptides, respectively). Regarding the inhibitory activity of angiotensin-I-converting enzyme (ACE), an IC<sub>50</sub> value of 111.33 ± 21.3 µg peptides/mL was observed in the PE. The identification of the peptide sequence of both hydrolysates was conducted, and LF and its PE presented 404 and 116 peptides, respectively, most with low molecular weight (<3 kDa), high percentage of hydrophobic amino acids, and typical characteristics of well-known antioxidant and ACE-inhibitory peptides. Furthermore, the potential bioactivity of the sequences identified was searched in the BIOPEP database. Considering the antioxidant and ACE-inhibitory activities, LF hydrolysates contained a larger number of sequences with potential bioactivity than PE hydrolysates.https://www.mdpi.com/2079-7737/11/5/631<i>Alphitobius diaperinus</i> L. larvaeangiotensin-I-converting enzyme (ACE) inhibitorantioxidantartichokebioactive peptide |
| spellingShingle | Luis Tejada Laura Buendía-Moreno Irene Hernández Adela Abellán José María Cayuela Eva Salazar Estefanía Bueno-Gavilá Bioactivities of Mealworm (<i>Alphitobius diaperinus</i> L.) Larvae Hydrolysates Obtained from Artichoke (<i>Cynara scolymus</i> L.) Proteases Biology <i>Alphitobius diaperinus</i> L. larvae angiotensin-I-converting enzyme (ACE) inhibitor antioxidant artichoke bioactive peptide |
| title | Bioactivities of Mealworm (<i>Alphitobius diaperinus</i> L.) Larvae Hydrolysates Obtained from Artichoke (<i>Cynara scolymus</i> L.) Proteases |
| title_full | Bioactivities of Mealworm (<i>Alphitobius diaperinus</i> L.) Larvae Hydrolysates Obtained from Artichoke (<i>Cynara scolymus</i> L.) Proteases |
| title_fullStr | Bioactivities of Mealworm (<i>Alphitobius diaperinus</i> L.) Larvae Hydrolysates Obtained from Artichoke (<i>Cynara scolymus</i> L.) Proteases |
| title_full_unstemmed | Bioactivities of Mealworm (<i>Alphitobius diaperinus</i> L.) Larvae Hydrolysates Obtained from Artichoke (<i>Cynara scolymus</i> L.) Proteases |
| title_short | Bioactivities of Mealworm (<i>Alphitobius diaperinus</i> L.) Larvae Hydrolysates Obtained from Artichoke (<i>Cynara scolymus</i> L.) Proteases |
| title_sort | bioactivities of mealworm i alphitobius diaperinus i l larvae hydrolysates obtained from artichoke i cynara scolymus i l proteases |
| topic | <i>Alphitobius diaperinus</i> L. larvae angiotensin-I-converting enzyme (ACE) inhibitor antioxidant artichoke bioactive peptide |
| url | https://www.mdpi.com/2079-7737/11/5/631 |
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