Standardization of in-house polymerase chain reaction for the identification of Mycobacterium tuberculosis at the reference tropical disease hospital in the State of Goiás, Brazil
This study compares smear, growth in Lowenstein-Jensen medium, and in-house polymerase chain reaction (PCR) techniques for the detection of Mycobacterium tuberculosis. A total of 72 specimens from 72 patients with clinical symptoms of tuberculosis, including 70 sputum and two bronchial aspirate samp...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2004-06-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000400012 |
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| author | Márcia Alves Vasconcelos Rodrigues Álvaro Bisol Serafini Marieta de Souza Pereira Thathiane Dias da Silva Marcelo Fouad Rabahi Suely Lemes de Alves André Kipnis |
| author_facet | Márcia Alves Vasconcelos Rodrigues Álvaro Bisol Serafini Marieta de Souza Pereira Thathiane Dias da Silva Marcelo Fouad Rabahi Suely Lemes de Alves André Kipnis |
| author_sort | Márcia Alves Vasconcelos Rodrigues |
| collection | DOAJ |
| description | This study compares smear, growth in Lowenstein-Jensen medium, and in-house polymerase chain reaction (PCR) techniques for the detection of Mycobacterium tuberculosis. A total of 72 specimens from 72 patients with clinical symptoms of tuberculosis, including 70 sputum and two bronchial aspirate samples, were tested in parallel by smear, culture, and in-house PCR techniques. From these, 48 (66.6%) were negative by the 3 methods, 2 (2.8%) were smear positive and negative by culture and in-house PCR, 11 (15.3%) were both smear and culture negative, and in-house PCR positive, 7 (9.7%) were positive by the 3 methods, 2 (2.8%) were positive by smear and culture, and negative by PCR, 2 (2.8%) were positive by culture and PCR, but smear negative. After the resolution of discrepancies in PCR results, the sensitivity and specificity for in-house PCR technique to M. tuberculosis relative to the culture, were 81.8% and 81.9%, respectively. These results confirm that this method, in-house PCR, may be a sensitive and specific technique for M. tuberculosis detection, occurring in both positive and negative smear and negative cultures. |
| first_indexed | 2024-03-12T08:11:58Z |
| format | Article |
| id | doaj.art-6ada84a146e44cba81f1b5ff1944c9e2 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:11:58Z |
| publishDate | 2004-06-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-6ada84a146e44cba81f1b5ff1944c9e22023-09-02T19:06:58ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602004-06-0199441541910.1590/S0074-02762004000400012Standardization of in-house polymerase chain reaction for the identification of Mycobacterium tuberculosis at the reference tropical disease hospital in the State of Goiás, BrazilMárcia Alves Vasconcelos RodriguesÁlvaro Bisol SerafiniMarieta de Souza PereiraThathiane Dias da SilvaMarcelo Fouad RabahiSuely Lemes de AlvesAndré KipnisThis study compares smear, growth in Lowenstein-Jensen medium, and in-house polymerase chain reaction (PCR) techniques for the detection of Mycobacterium tuberculosis. A total of 72 specimens from 72 patients with clinical symptoms of tuberculosis, including 70 sputum and two bronchial aspirate samples, were tested in parallel by smear, culture, and in-house PCR techniques. From these, 48 (66.6%) were negative by the 3 methods, 2 (2.8%) were smear positive and negative by culture and in-house PCR, 11 (15.3%) were both smear and culture negative, and in-house PCR positive, 7 (9.7%) were positive by the 3 methods, 2 (2.8%) were positive by smear and culture, and negative by PCR, 2 (2.8%) were positive by culture and PCR, but smear negative. After the resolution of discrepancies in PCR results, the sensitivity and specificity for in-house PCR technique to M. tuberculosis relative to the culture, were 81.8% and 81.9%, respectively. These results confirm that this method, in-house PCR, may be a sensitive and specific technique for M. tuberculosis detection, occurring in both positive and negative smear and negative cultures.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000400012Mycobacterium tuberculosispolymerase chain reactiontuberculosisculturesmear |
| spellingShingle | Márcia Alves Vasconcelos Rodrigues Álvaro Bisol Serafini Marieta de Souza Pereira Thathiane Dias da Silva Marcelo Fouad Rabahi Suely Lemes de Alves André Kipnis Standardization of in-house polymerase chain reaction for the identification of Mycobacterium tuberculosis at the reference tropical disease hospital in the State of Goiás, Brazil Memorias do Instituto Oswaldo Cruz Mycobacterium tuberculosis polymerase chain reaction tuberculosis culture smear |
| title | Standardization of in-house polymerase chain reaction for the identification of Mycobacterium tuberculosis at the reference tropical disease hospital in the State of Goiás, Brazil |
| title_full | Standardization of in-house polymerase chain reaction for the identification of Mycobacterium tuberculosis at the reference tropical disease hospital in the State of Goiás, Brazil |
| title_fullStr | Standardization of in-house polymerase chain reaction for the identification of Mycobacterium tuberculosis at the reference tropical disease hospital in the State of Goiás, Brazil |
| title_full_unstemmed | Standardization of in-house polymerase chain reaction for the identification of Mycobacterium tuberculosis at the reference tropical disease hospital in the State of Goiás, Brazil |
| title_short | Standardization of in-house polymerase chain reaction for the identification of Mycobacterium tuberculosis at the reference tropical disease hospital in the State of Goiás, Brazil |
| title_sort | standardization of in house polymerase chain reaction for the identification of mycobacterium tuberculosis at the reference tropical disease hospital in the state of goias brazil |
| topic | Mycobacterium tuberculosis polymerase chain reaction tuberculosis culture smear |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000400012 |
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