ROS-Mediated Fragmentation Alters the Effects of Hyaluronan on Corneal Epithelial Wound Healing
A buildup of reactive oxygen species (ROS) occurs in virtually all pathological conditions. Hyaluronan (HA) is a major extracellular matrix component and is susceptible to oxidation by reactive oxygen species (ROS), yet the precise chemical structures of oxidized HA products (oxHA) and their physiol...
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MDPI AG
2023-09-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/9/1385 |
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| author | Xiao Lin Isabel Y. Moreno Lawrence Nguyen Tarsis F. Gesteira Vivien J. Coulson-Thomas |
| author_facet | Xiao Lin Isabel Y. Moreno Lawrence Nguyen Tarsis F. Gesteira Vivien J. Coulson-Thomas |
| author_sort | Xiao Lin |
| collection | DOAJ |
| description | A buildup of reactive oxygen species (ROS) occurs in virtually all pathological conditions. Hyaluronan (HA) is a major extracellular matrix component and is susceptible to oxidation by reactive oxygen species (ROS), yet the precise chemical structures of oxidized HA products (oxHA) and their physiological properties remain largely unknown. This study characterized the molecular weight (MW), structures, and physiological properties of oxHA. For this, high-molecular-weight HA (HMWHA) was oxidized using increasing molar ratios of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) or hypochlorous acid (HOCl). ROS lead to the fragmentation of HA, with the oxHA products produced by HOCl exhibiting an altered chemical structure while those produced by H<sub>2</sub>O<sub>2</sub> do not. HMWHA promotes the viability of human corneal epithelial cells (hTCEpi), while low MWHA (LMWHA), ultra-LMWHA (ULMWHA), and most forms of oxHA do not. HMWHA and LMWHA promote hTCEpi proliferation, while ULMWHA and all forms of oxHA do not. LMWHA and some forms of oxHA promote hTCEpi migration, while HMWHA does not. Finally, all native forms of HA and oxHA produced by HOCl promote in vivo corneal wound healing, while oxHA produced by H<sub>2</sub>O<sub>2</sub> does not. Taken together, our results show that HA fragmentation by ROS can alter the physiological activity of HA by altering its MW and structure. |
| first_indexed | 2024-03-10T22:59:21Z |
| format | Article |
| id | doaj.art-6adcde4d5fb345f5b4f2414e08b9ab7b |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T22:59:21Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-6adcde4d5fb345f5b4f2414e08b9ab7b2023-11-19T09:46:15ZengMDPI AGBiomolecules2218-273X2023-09-01139138510.3390/biom13091385ROS-Mediated Fragmentation Alters the Effects of Hyaluronan on Corneal Epithelial Wound HealingXiao Lin0Isabel Y. Moreno1Lawrence Nguyen2Tarsis F. Gesteira3Vivien J. Coulson-Thomas4College of Optometry, University of Houston, 4401 Martin Luther King Boulevard, Houston, TX 77204-2020, USACollege of Optometry, University of Houston, 4401 Martin Luther King Boulevard, Houston, TX 77204-2020, USACollege of Optometry, University of Houston, 4401 Martin Luther King Boulevard, Houston, TX 77204-2020, USACollege of Optometry, University of Houston, 4401 Martin Luther King Boulevard, Houston, TX 77204-2020, USACollege of Optometry, University of Houston, 4401 Martin Luther King Boulevard, Houston, TX 77204-2020, USAA buildup of reactive oxygen species (ROS) occurs in virtually all pathological conditions. Hyaluronan (HA) is a major extracellular matrix component and is susceptible to oxidation by reactive oxygen species (ROS), yet the precise chemical structures of oxidized HA products (oxHA) and their physiological properties remain largely unknown. This study characterized the molecular weight (MW), structures, and physiological properties of oxHA. For this, high-molecular-weight HA (HMWHA) was oxidized using increasing molar ratios of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) or hypochlorous acid (HOCl). ROS lead to the fragmentation of HA, with the oxHA products produced by HOCl exhibiting an altered chemical structure while those produced by H<sub>2</sub>O<sub>2</sub> do not. HMWHA promotes the viability of human corneal epithelial cells (hTCEpi), while low MWHA (LMWHA), ultra-LMWHA (ULMWHA), and most forms of oxHA do not. HMWHA and LMWHA promote hTCEpi proliferation, while ULMWHA and all forms of oxHA do not. LMWHA and some forms of oxHA promote hTCEpi migration, while HMWHA does not. Finally, all native forms of HA and oxHA produced by HOCl promote in vivo corneal wound healing, while oxHA produced by H<sub>2</sub>O<sub>2</sub> does not. Taken together, our results show that HA fragmentation by ROS can alter the physiological activity of HA by altering its MW and structure.https://www.mdpi.com/2218-273X/13/9/1385high-molecular-weight hyaluronanlow-molecular-weight hyaluronanoxidative stresswound healingcorneal epithelium |
| spellingShingle | Xiao Lin Isabel Y. Moreno Lawrence Nguyen Tarsis F. Gesteira Vivien J. Coulson-Thomas ROS-Mediated Fragmentation Alters the Effects of Hyaluronan on Corneal Epithelial Wound Healing Biomolecules high-molecular-weight hyaluronan low-molecular-weight hyaluronan oxidative stress wound healing corneal epithelium |
| title | ROS-Mediated Fragmentation Alters the Effects of Hyaluronan on Corneal Epithelial Wound Healing |
| title_full | ROS-Mediated Fragmentation Alters the Effects of Hyaluronan on Corneal Epithelial Wound Healing |
| title_fullStr | ROS-Mediated Fragmentation Alters the Effects of Hyaluronan on Corneal Epithelial Wound Healing |
| title_full_unstemmed | ROS-Mediated Fragmentation Alters the Effects of Hyaluronan on Corneal Epithelial Wound Healing |
| title_short | ROS-Mediated Fragmentation Alters the Effects of Hyaluronan on Corneal Epithelial Wound Healing |
| title_sort | ros mediated fragmentation alters the effects of hyaluronan on corneal epithelial wound healing |
| topic | high-molecular-weight hyaluronan low-molecular-weight hyaluronan oxidative stress wound healing corneal epithelium |
| url | https://www.mdpi.com/2218-273X/13/9/1385 |
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