Zingiber zerumbet L. (Smith) extract alleviates the ethanol-induced brain damage via its antioxidant activity
Abstract Background Zingiber zerumbet (L.) Smith belongs to the Zingiberaceae family that is widely distributed throughout the tropics, particularly in Southeast Asia. It is locally known as ‘Lempoyang’ and traditionally used to treat fever, constipation and to relieve pain. It is also known to poss...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-03-01
|
| Series: | BMC Complementary and Alternative Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12906-018-2161-5 |
| _version_ | 1819016774901825536 |
|---|---|
| author | Asmah Hamid Farah Wahida Ibrahim Teoh Hooi Ming Mohd Nazir Nasrom Norelina Eusoff Khairana Husain Mazlyzam Abdul Latif |
| author_facet | Asmah Hamid Farah Wahida Ibrahim Teoh Hooi Ming Mohd Nazir Nasrom Norelina Eusoff Khairana Husain Mazlyzam Abdul Latif |
| author_sort | Asmah Hamid |
| collection | DOAJ |
| description | Abstract Background Zingiber zerumbet (L.) Smith belongs to the Zingiberaceae family that is widely distributed throughout the tropics, particularly in Southeast Asia. It is locally known as ‘Lempoyang’ and traditionally used to treat fever, constipation and to relieve pain. It is also known to possess antioxidant and anti-inflammatory activities. Based on these antioxidant and anti-inflammatory activities, this study was conducted to investigate the effects of ethyl-acetate extract of Z. zerumbet rhizomes against ethanol-induced brain damage in male Wistar rats. Method Twenty-four male Wistar rats were divided into four groups which consist of normal, 1.8 g/kg ethanol (40% v/v), 200 mg/kg Z. zerumbet extract plus ethanol and 400 mg/kg Z. zerumbet plus ethanol. The extract of Z. zerumbet was given once daily by oral gavage, 30 min prior to ethanol exposure via intraperitoneal route for 14 consecutive days. The rats were then sacrificed. Blood and brain homogenate were subjected to biochemical tests and part of the brain tissue was sectioned for histological analysis. Result Treatment with ethyl-acetate Z. zerumbet extract at 200 mg/kg and 400 mg/kg significantly reduced the level of malondialdehyde (MDA) and protein carbonyl (p < 0.05) in the brain homogenate. Both doses of extracts also significantly increased the level of serum superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities as well as glutathione (GSH) level (p < 0.05). However, administration of ethyl-acetate Z. zerumbet extract at 400 mg/kg showed better protective effects on the ethanol-induced brain damage as shown with higher levels of SOD, CAT, GPx and GSH in the brain homogenate as compared to 200 mg/kg dose. Histological observation of the cerebellum and cerebral cortex showed that the extract prevented the loss of Purkinje cells and retained the number and the shape of the cells. Conclusion Ethyl-acetate extract of Z. zerumbet has protective effects against ethanol-induced brain damage and this is mediated through its antioxidant properties. Graphical abstract Z. zerumbet extract protects against ethanol-induced brain damage via its antioxidant properties |
| first_indexed | 2024-12-21T02:52:59Z |
| format | Article |
| id | doaj.art-6ade3ee973994cac9f95c48a0a2de844 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-21T02:52:59Z |
| publishDate | 2018-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-6ade3ee973994cac9f95c48a0a2de8442022-12-21T19:18:24ZengBMCBMC Complementary and Alternative Medicine1472-68822018-03-011811910.1186/s12906-018-2161-5Zingiber zerumbet L. (Smith) extract alleviates the ethanol-induced brain damage via its antioxidant activityAsmah Hamid0Farah Wahida Ibrahim1Teoh Hooi Ming2Mohd Nazir Nasrom3Norelina Eusoff4Khairana Husain5Mazlyzam Abdul Latif6Biomedical Science Programme, Centre of Health & Applied Sciences, Faculty of Health Sciences, Universiti Kebangsaan MalaysiaBiomedical Science Programme, Centre of Health & Applied Sciences, Faculty of Health Sciences, Universiti Kebangsaan MalaysiaBiomedical Science Programme, Centre of Health & Applied Sciences, Faculty of Health Sciences, Universiti Kebangsaan MalaysiaBiomedical Science Programme, Centre of Health & Applied Sciences, Faculty of Health Sciences, Universiti Kebangsaan MalaysiaBiomedical Science Programme, Centre of Health & Applied Sciences, Faculty of Health Sciences, Universiti Kebangsaan MalaysiaFaculty of Pharmacy, Universiti Kebangsaan MalaysiaBiomedical Science Programme, Centre of Health & Applied Sciences, Faculty of Health Sciences, Universiti Kebangsaan MalaysiaAbstract Background Zingiber zerumbet (L.) Smith belongs to the Zingiberaceae family that is widely distributed throughout the tropics, particularly in Southeast Asia. It is locally known as ‘Lempoyang’ and traditionally used to treat fever, constipation and to relieve pain. It is also known to possess antioxidant and anti-inflammatory activities. Based on these antioxidant and anti-inflammatory activities, this study was conducted to investigate the effects of ethyl-acetate extract of Z. zerumbet rhizomes against ethanol-induced brain damage in male Wistar rats. Method Twenty-four male Wistar rats were divided into four groups which consist of normal, 1.8 g/kg ethanol (40% v/v), 200 mg/kg Z. zerumbet extract plus ethanol and 400 mg/kg Z. zerumbet plus ethanol. The extract of Z. zerumbet was given once daily by oral gavage, 30 min prior to ethanol exposure via intraperitoneal route for 14 consecutive days. The rats were then sacrificed. Blood and brain homogenate were subjected to biochemical tests and part of the brain tissue was sectioned for histological analysis. Result Treatment with ethyl-acetate Z. zerumbet extract at 200 mg/kg and 400 mg/kg significantly reduced the level of malondialdehyde (MDA) and protein carbonyl (p < 0.05) in the brain homogenate. Both doses of extracts also significantly increased the level of serum superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities as well as glutathione (GSH) level (p < 0.05). However, administration of ethyl-acetate Z. zerumbet extract at 400 mg/kg showed better protective effects on the ethanol-induced brain damage as shown with higher levels of SOD, CAT, GPx and GSH in the brain homogenate as compared to 200 mg/kg dose. Histological observation of the cerebellum and cerebral cortex showed that the extract prevented the loss of Purkinje cells and retained the number and the shape of the cells. Conclusion Ethyl-acetate extract of Z. zerumbet has protective effects against ethanol-induced brain damage and this is mediated through its antioxidant properties. Graphical abstract Z. zerumbet extract protects against ethanol-induced brain damage via its antioxidant propertieshttp://link.springer.com/article/10.1186/s12906-018-2161-5Zingiber zerumbetEthanolBrain damageAntioxidantOxidative stress |
| spellingShingle | Asmah Hamid Farah Wahida Ibrahim Teoh Hooi Ming Mohd Nazir Nasrom Norelina Eusoff Khairana Husain Mazlyzam Abdul Latif Zingiber zerumbet L. (Smith) extract alleviates the ethanol-induced brain damage via its antioxidant activity BMC Complementary and Alternative Medicine Zingiber zerumbet Ethanol Brain damage Antioxidant Oxidative stress |
| title | Zingiber zerumbet L. (Smith) extract alleviates the ethanol-induced brain damage via its antioxidant activity |
| title_full | Zingiber zerumbet L. (Smith) extract alleviates the ethanol-induced brain damage via its antioxidant activity |
| title_fullStr | Zingiber zerumbet L. (Smith) extract alleviates the ethanol-induced brain damage via its antioxidant activity |
| title_full_unstemmed | Zingiber zerumbet L. (Smith) extract alleviates the ethanol-induced brain damage via its antioxidant activity |
| title_short | Zingiber zerumbet L. (Smith) extract alleviates the ethanol-induced brain damage via its antioxidant activity |
| title_sort | zingiber zerumbet l smith extract alleviates the ethanol induced brain damage via its antioxidant activity |
| topic | Zingiber zerumbet Ethanol Brain damage Antioxidant Oxidative stress |
| url | http://link.springer.com/article/10.1186/s12906-018-2161-5 |
| work_keys_str_mv | AT asmahhamid zingiberzerumbetlsmithextractalleviatestheethanolinducedbraindamageviaitsantioxidantactivity AT farahwahidaibrahim zingiberzerumbetlsmithextractalleviatestheethanolinducedbraindamageviaitsantioxidantactivity AT teohhooiming zingiberzerumbetlsmithextractalleviatestheethanolinducedbraindamageviaitsantioxidantactivity AT mohdnazirnasrom zingiberzerumbetlsmithextractalleviatestheethanolinducedbraindamageviaitsantioxidantactivity AT norelinaeusoff zingiberzerumbetlsmithextractalleviatestheethanolinducedbraindamageviaitsantioxidantactivity AT khairanahusain zingiberzerumbetlsmithextractalleviatestheethanolinducedbraindamageviaitsantioxidantactivity AT mazlyzamabdullatif zingiberzerumbetlsmithextractalleviatestheethanolinducedbraindamageviaitsantioxidantactivity |