Traditional Tibetan Medicine Twenty-Five Wei’er Tea Pills Ameliorate Rheumatoid Arthritis Based on Chemical Crosstalk Between Gut Microbiota and the Host
Twenty-Five Wei’er Tea Pills (TFP), a traditional Tibetan medicine, has shown to have a promising therapeutic effect in patients with Rheumatoid arthritis (RA), as well as being safe. Nonetheless, there have been limited pharmacological studies that have explored this therapeutic option. As gut micr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.828920/full |
| _version_ | 1818897480572469248 |
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| author | Zixuan Li Lijuan Nie Yong Li Lu Yang Lulu Jin Baozhong Du Juan Yang Xulin Zhang Huantian Cui Ouzhu Luobu Ouzhu Luobu |
| author_facet | Zixuan Li Lijuan Nie Yong Li Lu Yang Lulu Jin Baozhong Du Juan Yang Xulin Zhang Huantian Cui Ouzhu Luobu Ouzhu Luobu |
| author_sort | Zixuan Li |
| collection | DOAJ |
| description | Twenty-Five Wei’er Tea Pills (TFP), a traditional Tibetan medicine, has shown to have a promising therapeutic effect in patients with Rheumatoid arthritis (RA), as well as being safe. Nonetheless, there have been limited pharmacological studies that have explored this therapeutic option. As gut microbiota has been proven to have a critical role in the pathogenesis of RA, this study aims to explore and reveal relevant ways by which TFP interacts with the chemical crosstalk between the gut microbiome and its host. 16S rRNA sequencing, combined with un-targeted metabolomics, were conducted on collagen-induced arthritis (CIA) rats. CIA model rats treated with TFP showed significant improvement in weight gain, pathological phenomena in joints, as well as decreased serum levels of TNF-α, IL-6 and increased level of IL-4 and IL-10. Significant dysfunction in the gut microbiome and alteration in serum metabolites were observed in CIA model rats, which were restored by TFP treatment. Coherence analysis indicated that TFP modulated the pathways of histidine metabolism, phenylalanine metabolism, alanine, aspartate, glutamate metabolism, amino sugar and nucleotide sugar metabolism owing to the abundances of Lactobacillus, Bacteroides, Prevotellaceae_UCG-001 and Christensenellaceae_R-7_group in the gut microflora. The corresponding metabolites involved L-histidine, histamine, phenylethylamine, asparagine, L-aspartic acid, D-fructose 1-phosphate, D-Mannose 6-phosphate, D-Glucose 6-phosphate, and Glucose 1-phosphate. In conclusion, this study reveals the ameliorative effects of TFP on RA through the chemical crosstalk that exists between the gut microbiota and its host, and also further enriches our understandings of the pathogenesis of RA. |
| first_indexed | 2024-12-19T19:16:51Z |
| format | Article |
| id | doaj.art-6ae00d9859fb4360b06f3af13d354694 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-19T19:16:51Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-6ae00d9859fb4360b06f3af13d3546942022-12-21T20:09:07ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-02-011310.3389/fphar.2022.828920828920Traditional Tibetan Medicine Twenty-Five Wei’er Tea Pills Ameliorate Rheumatoid Arthritis Based on Chemical Crosstalk Between Gut Microbiota and the HostZixuan Li0Lijuan Nie1Yong Li2Lu Yang3Lulu Jin4Baozhong Du5Juan Yang6Xulin Zhang7Huantian Cui8Ouzhu Luobu9Ouzhu Luobu10Department of Basic Medicine, Medical College of Tibet University, Lhasa, ChinaDepartment of Pharmacy, Medical College of Tibet University, Lhasa, ChinaInstitute of Oxygen Supply, Center of Tibetan Studies (Everest Research Institute), Tibet University, Lhasa, ChinaTianjin University of Traditional Chinese Medicine, Tianjin, ChinaTianjin University of Traditional Chinese Medicine, Tianjin, ChinaDepartment of Basic Medicine, Medical College of Tibet University, Lhasa, ChinaDepartment of Basic Medicine, Medical College of Tibet University, Lhasa, ChinaSecond Affiliated Hospital of University of South China, Hengyang, ChinaShandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, ChinaMedical College of Tibet University, Lhasa, ChinaAffiliated Fukang Hospital of Tibet University, Lhasa, ChinaTwenty-Five Wei’er Tea Pills (TFP), a traditional Tibetan medicine, has shown to have a promising therapeutic effect in patients with Rheumatoid arthritis (RA), as well as being safe. Nonetheless, there have been limited pharmacological studies that have explored this therapeutic option. As gut microbiota has been proven to have a critical role in the pathogenesis of RA, this study aims to explore and reveal relevant ways by which TFP interacts with the chemical crosstalk between the gut microbiome and its host. 16S rRNA sequencing, combined with un-targeted metabolomics, were conducted on collagen-induced arthritis (CIA) rats. CIA model rats treated with TFP showed significant improvement in weight gain, pathological phenomena in joints, as well as decreased serum levels of TNF-α, IL-6 and increased level of IL-4 and IL-10. Significant dysfunction in the gut microbiome and alteration in serum metabolites were observed in CIA model rats, which were restored by TFP treatment. Coherence analysis indicated that TFP modulated the pathways of histidine metabolism, phenylalanine metabolism, alanine, aspartate, glutamate metabolism, amino sugar and nucleotide sugar metabolism owing to the abundances of Lactobacillus, Bacteroides, Prevotellaceae_UCG-001 and Christensenellaceae_R-7_group in the gut microflora. The corresponding metabolites involved L-histidine, histamine, phenylethylamine, asparagine, L-aspartic acid, D-fructose 1-phosphate, D-Mannose 6-phosphate, D-Glucose 6-phosphate, and Glucose 1-phosphate. In conclusion, this study reveals the ameliorative effects of TFP on RA through the chemical crosstalk that exists between the gut microbiota and its host, and also further enriches our understandings of the pathogenesis of RA.https://www.frontiersin.org/articles/10.3389/fphar.2022.828920/fullTibetan medicinetwenty-five Wei’er tea pillsrheumatoid arthritisgut microbiotametabolomics |
| spellingShingle | Zixuan Li Lijuan Nie Yong Li Lu Yang Lulu Jin Baozhong Du Juan Yang Xulin Zhang Huantian Cui Ouzhu Luobu Ouzhu Luobu Traditional Tibetan Medicine Twenty-Five Wei’er Tea Pills Ameliorate Rheumatoid Arthritis Based on Chemical Crosstalk Between Gut Microbiota and the Host Frontiers in Pharmacology Tibetan medicine twenty-five Wei’er tea pills rheumatoid arthritis gut microbiota metabolomics |
| title | Traditional Tibetan Medicine Twenty-Five Wei’er Tea Pills Ameliorate Rheumatoid Arthritis Based on Chemical Crosstalk Between Gut Microbiota and the Host |
| title_full | Traditional Tibetan Medicine Twenty-Five Wei’er Tea Pills Ameliorate Rheumatoid Arthritis Based on Chemical Crosstalk Between Gut Microbiota and the Host |
| title_fullStr | Traditional Tibetan Medicine Twenty-Five Wei’er Tea Pills Ameliorate Rheumatoid Arthritis Based on Chemical Crosstalk Between Gut Microbiota and the Host |
| title_full_unstemmed | Traditional Tibetan Medicine Twenty-Five Wei’er Tea Pills Ameliorate Rheumatoid Arthritis Based on Chemical Crosstalk Between Gut Microbiota and the Host |
| title_short | Traditional Tibetan Medicine Twenty-Five Wei’er Tea Pills Ameliorate Rheumatoid Arthritis Based on Chemical Crosstalk Between Gut Microbiota and the Host |
| title_sort | traditional tibetan medicine twenty five wei er tea pills ameliorate rheumatoid arthritis based on chemical crosstalk between gut microbiota and the host |
| topic | Tibetan medicine twenty-five Wei’er tea pills rheumatoid arthritis gut microbiota metabolomics |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.828920/full |
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