The blue light signal transduction module FaCRY1-FaCOP1-FaHY5 regulates anthocyanin accumulation in cultivated strawberry
Anthocyanins have important physiological functions and are beneficial to the improvement of fruit quality in strawberry. Light is important for anthocyanin biosynthesis, and specific light quality was identified to promote anthocyanin accumulation in many fruits. However, research on the molecular...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1144273/full |
| _version_ | 1797808334304182272 |
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| author | Yongqiang Liu Yongqiang Liu Li Tang Yiping Wang Lianxi Zhang Shiqiong Xu Xiao Wang Wen He Yunting Zhang Yuanxiu Lin Yan Wang Mengyao Li Xiaorong Wang Yong Zhang Ya Luo Qing Chen Haoru Tang Haoru Tang |
| author_facet | Yongqiang Liu Yongqiang Liu Li Tang Yiping Wang Lianxi Zhang Shiqiong Xu Xiao Wang Wen He Yunting Zhang Yuanxiu Lin Yan Wang Mengyao Li Xiaorong Wang Yong Zhang Ya Luo Qing Chen Haoru Tang Haoru Tang |
| author_sort | Yongqiang Liu |
| collection | DOAJ |
| description | Anthocyanins have important physiological functions and are beneficial to the improvement of fruit quality in strawberry. Light is important for anthocyanin biosynthesis, and specific light quality was identified to promote anthocyanin accumulation in many fruits. However, research on the molecular mechanisms of anthocyanin accumulation regulated by light quality in strawberry remains limited. Here we described the effects of red- and blue-light irradiation on anthocyanin accumulation in strawberry. The results showed that blue light, rather than red light, could lead to the rapid accumulation of anthocyanins after exposure to light for 48 hours. The transcriptional levels of anthocyanin structural and regulatory genes displayed similar trend to the anthocyanin content. To investigate the mechanism of blue light-induced anthocyanin accumulation, the homologs of Arabidopsis blue light signal transduction components, including the blue light photoreceptor FaCRY1, an E3 ubiquitin ligase FaCOP1 and light-responsive factor FaHY5, were cloned from the strawberry cultivar ‘Benihoppe’. The protein-protein interaction of FaCRY1-FaCOP1-FaHY5 was revealed by yeast two-hybrid and fluorescence signal assays. Functional complementation analysis showed that overexpression of either FaCOP1 or FaHY5 restored the anthocyanin content and hypocotyl length in corresponding Arabidopsis mutants under blue light. Moreover, dual-luciferase assays showed that FaHY5 could increase the activity of FaRAP (anthocyanin transport gene) promoter and that this function relied on other, likely B-box protein FaBBX22, factors. The overexpression of FaHY5-VP16 (chimeric activator form of FaHY5) and FaBBX22 promoted the accumulation of anthocyanins in transgenic strawberry plants. Further, transcriptomic profiling indicated that the genes involved in the phenylpropanoid biosynthesis pathway were enriched in both FaHY5-VP16-OX and FaBBX22-OX strawberry plants. In summary, our findings provide insights into a mechanism involving the regulation of blue light-induced anthocyanin accumulation via a FaCRY1-FaCOP1-FaHY5 signal transduction module in strawberry. |
| first_indexed | 2024-03-13T06:36:00Z |
| format | Article |
| id | doaj.art-6ae990fc9cd247c08735ef0923302732 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-13T06:36:00Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-6ae990fc9cd247c08735ef09233027322023-06-09T05:11:29ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-06-011410.3389/fpls.2023.11442731144273The blue light signal transduction module FaCRY1-FaCOP1-FaHY5 regulates anthocyanin accumulation in cultivated strawberryYongqiang Liu0Yongqiang Liu1Li Tang2Yiping Wang3Lianxi Zhang4Shiqiong Xu5Xiao Wang6Wen He7Yunting Zhang8Yuanxiu Lin9Yan Wang10Mengyao Li11Xiaorong Wang12Yong Zhang13Ya Luo14Qing Chen15Haoru Tang16Haoru Tang17College of Horticulture, Sichuan Agricultural University, Chengdu, ChinaInstitute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu, ChinaInstitute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, ChinaInstitute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, ChinaInstitute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, ChinaInstitute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu, ChinaInstitute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu, ChinaInstitute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, ChinaAnthocyanins have important physiological functions and are beneficial to the improvement of fruit quality in strawberry. Light is important for anthocyanin biosynthesis, and specific light quality was identified to promote anthocyanin accumulation in many fruits. However, research on the molecular mechanisms of anthocyanin accumulation regulated by light quality in strawberry remains limited. Here we described the effects of red- and blue-light irradiation on anthocyanin accumulation in strawberry. The results showed that blue light, rather than red light, could lead to the rapid accumulation of anthocyanins after exposure to light for 48 hours. The transcriptional levels of anthocyanin structural and regulatory genes displayed similar trend to the anthocyanin content. To investigate the mechanism of blue light-induced anthocyanin accumulation, the homologs of Arabidopsis blue light signal transduction components, including the blue light photoreceptor FaCRY1, an E3 ubiquitin ligase FaCOP1 and light-responsive factor FaHY5, were cloned from the strawberry cultivar ‘Benihoppe’. The protein-protein interaction of FaCRY1-FaCOP1-FaHY5 was revealed by yeast two-hybrid and fluorescence signal assays. Functional complementation analysis showed that overexpression of either FaCOP1 or FaHY5 restored the anthocyanin content and hypocotyl length in corresponding Arabidopsis mutants under blue light. Moreover, dual-luciferase assays showed that FaHY5 could increase the activity of FaRAP (anthocyanin transport gene) promoter and that this function relied on other, likely B-box protein FaBBX22, factors. The overexpression of FaHY5-VP16 (chimeric activator form of FaHY5) and FaBBX22 promoted the accumulation of anthocyanins in transgenic strawberry plants. Further, transcriptomic profiling indicated that the genes involved in the phenylpropanoid biosynthesis pathway were enriched in both FaHY5-VP16-OX and FaBBX22-OX strawberry plants. In summary, our findings provide insights into a mechanism involving the regulation of blue light-induced anthocyanin accumulation via a FaCRY1-FaCOP1-FaHY5 signal transduction module in strawberry.https://www.frontiersin.org/articles/10.3389/fpls.2023.1144273/fullstrawberryanthocyaninblue lightHY5COP1BBX protein |
| spellingShingle | Yongqiang Liu Yongqiang Liu Li Tang Yiping Wang Lianxi Zhang Shiqiong Xu Xiao Wang Wen He Yunting Zhang Yuanxiu Lin Yan Wang Mengyao Li Xiaorong Wang Yong Zhang Ya Luo Qing Chen Haoru Tang Haoru Tang The blue light signal transduction module FaCRY1-FaCOP1-FaHY5 regulates anthocyanin accumulation in cultivated strawberry Frontiers in Plant Science strawberry anthocyanin blue light HY5 COP1 BBX protein |
| title | The blue light signal transduction module FaCRY1-FaCOP1-FaHY5 regulates anthocyanin accumulation in cultivated strawberry |
| title_full | The blue light signal transduction module FaCRY1-FaCOP1-FaHY5 regulates anthocyanin accumulation in cultivated strawberry |
| title_fullStr | The blue light signal transduction module FaCRY1-FaCOP1-FaHY5 regulates anthocyanin accumulation in cultivated strawberry |
| title_full_unstemmed | The blue light signal transduction module FaCRY1-FaCOP1-FaHY5 regulates anthocyanin accumulation in cultivated strawberry |
| title_short | The blue light signal transduction module FaCRY1-FaCOP1-FaHY5 regulates anthocyanin accumulation in cultivated strawberry |
| title_sort | blue light signal transduction module facry1 facop1 fahy5 regulates anthocyanin accumulation in cultivated strawberry |
| topic | strawberry anthocyanin blue light HY5 COP1 BBX protein |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1144273/full |
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