Metallothionein Cup1 attenuates nitrosative stress in the yeast Saccharomyces cerevisiae
Metallothionein (MT), which is a small metal-binding protein with cysteine-rich motifs, functions in the detoxification of heavy metals in a variety of organisms. Even though previous studies suggest that MT is in-volved in the tolerance mechanisms against nitrosative stress induced by toxic levels...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Shared Science Publishers OG
2022-07-01
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| Series: | Microbial Cell |
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| Online Access: | http://microbialcell.com/researcharticles/2023a-yoshikawa-microbial-cell/ |
| _version_ | 1826531970650210304 |
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| author | Yuki Yoshikawa Ryo Nasuno Naoki Takaya Hiroshi Takagi |
| author_facet | Yuki Yoshikawa Ryo Nasuno Naoki Takaya Hiroshi Takagi |
| author_sort | Yuki Yoshikawa |
| collection | DOAJ |
| description | Metallothionein (MT), which is a small metal-binding protein with cysteine-rich motifs, functions in the detoxification of heavy metals in a variety of organisms. Even though previous studies suggest that MT is in-volved in the tolerance mechanisms against nitrosative stress induced by toxic levels of nitric oxide (NO) in mammalian cells, the physiological functions of MT in relation to NO have not been fully understood. In this study, we analyzed the functions of MT in nitrosative stress tolerance in the yeast Saccharomyces cerevisiae. Our phenotypic analyses showed that deletion or overexpression of the MT-encoding gene, CUP1, led to higher sensitivity or tolerance to nitrosative stress in S. cerevisiae cells, respectively. We further examined whether the yeast MT Cup1 in the cell-free lysate scavenges NO. These results showed that the cell-free lysate containing a higher level of Cup1 degraded NO more efficiently. On the other hand, the transcription level of CUP1 was not affected by nitrosative stress treatment. Our findings suggest that the yeast MT Cup1 contributes to nitrosative stress tolerance, possibly as a constitutive rather than an inducible defense mechanism. |
| first_indexed | 2024-03-12T20:35:13Z |
| format | Article |
| id | doaj.art-6aeb16eb2a84451db8526a4bc88018e4 |
| institution | Directory Open Access Journal |
| issn | 2311-2638 |
| language | English |
| last_indexed | 2025-03-14T01:43:48Z |
| publishDate | 2022-07-01 |
| publisher | Shared Science Publishers OG |
| record_format | Article |
| series | Microbial Cell |
| spelling | doaj.art-6aeb16eb2a84451db8526a4bc88018e42025-03-12T13:46:45ZengShared Science Publishers OGMicrobial Cell2311-26382022-07-0110817017710.15698/mic2023.08.802Metallothionein Cup1 attenuates nitrosative stress in the yeast Saccharomyces cerevisiaeYuki Yoshikawa0Ryo Nasuno1Naoki Takaya2Hiroshi Takagi3Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.Faculty of Life and Environmental Sciences, Microbiology Research Center for Sustainability, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.Metallothionein (MT), which is a small metal-binding protein with cysteine-rich motifs, functions in the detoxification of heavy metals in a variety of organisms. Even though previous studies suggest that MT is in-volved in the tolerance mechanisms against nitrosative stress induced by toxic levels of nitric oxide (NO) in mammalian cells, the physiological functions of MT in relation to NO have not been fully understood. In this study, we analyzed the functions of MT in nitrosative stress tolerance in the yeast Saccharomyces cerevisiae. Our phenotypic analyses showed that deletion or overexpression of the MT-encoding gene, CUP1, led to higher sensitivity or tolerance to nitrosative stress in S. cerevisiae cells, respectively. We further examined whether the yeast MT Cup1 in the cell-free lysate scavenges NO. These results showed that the cell-free lysate containing a higher level of Cup1 degraded NO more efficiently. On the other hand, the transcription level of CUP1 was not affected by nitrosative stress treatment. Our findings suggest that the yeast MT Cup1 contributes to nitrosative stress tolerance, possibly as a constitutive rather than an inducible defense mechanism.http://microbialcell.com/researcharticles/2023a-yoshikawa-microbial-cell/metallothioneinnitrosative stressyeastfission yeaststress tolerance |
| spellingShingle | Yuki Yoshikawa Ryo Nasuno Naoki Takaya Hiroshi Takagi Metallothionein Cup1 attenuates nitrosative stress in the yeast Saccharomyces cerevisiae Microbial Cell metallothionein nitrosative stress yeast fission yeast stress tolerance |
| title | Metallothionein Cup1 attenuates nitrosative stress in the yeast Saccharomyces cerevisiae |
| title_full | Metallothionein Cup1 attenuates nitrosative stress in the yeast Saccharomyces cerevisiae |
| title_fullStr | Metallothionein Cup1 attenuates nitrosative stress in the yeast Saccharomyces cerevisiae |
| title_full_unstemmed | Metallothionein Cup1 attenuates nitrosative stress in the yeast Saccharomyces cerevisiae |
| title_short | Metallothionein Cup1 attenuates nitrosative stress in the yeast Saccharomyces cerevisiae |
| title_sort | metallothionein cup1 attenuates nitrosative stress in the yeast saccharomyces cerevisiae |
| topic | metallothionein nitrosative stress yeast fission yeast stress tolerance |
| url | http://microbialcell.com/researcharticles/2023a-yoshikawa-microbial-cell/ |
| work_keys_str_mv | AT yukiyoshikawa metallothioneincup1attenuatesnitrosativestressintheyeastsaccharomycescerevisiae AT ryonasuno metallothioneincup1attenuatesnitrosativestressintheyeastsaccharomycescerevisiae AT naokitakaya metallothioneincup1attenuatesnitrosativestressintheyeastsaccharomycescerevisiae AT hiroshitakagi metallothioneincup1attenuatesnitrosativestressintheyeastsaccharomycescerevisiae |