Anti-Herpes Simplex virus and antibacterial activities of Graptopetalum paraguayense E. Walther leaf extract: a pilot study
Graptopetalum paraguayense E. Walther (GP) belongs to the Crassulaceae family and has several health benefits. In the present study we evaluated the cytotoxic, anti-Herpes Simplex virus (HSV) and antibacterial activities of a total methanol/water GP extract. The results from the cytotoxicity investi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13102818.2019.1656108 |
| _version_ | 1818912039515455488 |
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| author | Maya Margaritova Zaharieva Petia Genova-Kalоu Ivayla Dincheva Ilian Badjakov Stefka Krumova Venelin Enchev Hristo Najdenski Nadezhda Markova |
| author_facet | Maya Margaritova Zaharieva Petia Genova-Kalоu Ivayla Dincheva Ilian Badjakov Stefka Krumova Venelin Enchev Hristo Najdenski Nadezhda Markova |
| author_sort | Maya Margaritova Zaharieva |
| collection | DOAJ |
| description | Graptopetalum paraguayense E. Walther (GP) belongs to the Crassulaceae family and has several health benefits. In the present study we evaluated the cytotoxic, anti-Herpes Simplex virus (HSV) and antibacterial activities of a total methanol/water GP extract. The results from the cytotoxicity investigation of the tested extract showed a high cell tolerable concentration range. The GP extract demonstrated a significant inhibitory effect on the wild-type HSV-1 strain Victoria in a concentration of 0.0001 mg/mL equal to the maximal nontoxic concentration (with 97.5% protection of the cells). Regarding the acyclovir-resistant mutant HSV-2 strain PU and wild-type HSV-2 strain Bja, the antiviral activity and selectivity of the extract were very low, with EC50 values from 0.1 to 0.01 mg/mL, and the selectivity index ranging from 25 to 250, respectively. The GP extract exhibited selective inhibitory activity also on the growth of Gram-positive bacterial pathogens, with Staphylococcus aureus being most sensitive. It also prevented the formation of MRSA biofilm up to 50% at a concentration of 1.6 mg/mL and up to 90% at a concentration of 2.5 mg/mL. The anti-herpetic and antibacterial activities demonstrated in our investigation are probably related to the presence of phenols, and in particular gallic acid. The antimicrobial activity of the GP extract holds a promise for the future development of nutrition supplements based on G. paraguayense and therefore warrants further studies aimed at isolating biologically active compounds or mixtures thereof with subsequent in-depth investigation of their anti-HSV and antibacterial mechanisms of action. |
| first_indexed | 2024-12-19T23:08:15Z |
| format | Article |
| id | doaj.art-6aeb7b90daff411e835e2cb3d2ecb0aa |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-19T23:08:15Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-6aeb7b90daff411e835e2cb3d2ecb0aa2022-12-21T20:02:19ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302019-01-013311251125910.1080/13102818.2019.16561081656108Anti-Herpes Simplex virus and antibacterial activities of Graptopetalum paraguayense E. Walther leaf extract: a pilot studyMaya Margaritova Zaharieva0Petia Genova-Kalоu1Ivayla Dincheva2Ilian Badjakov3Stefka Krumova4Venelin Enchev5Hristo Najdenski6Nadezhda Markova7Bulgarian Academy of SciencesNational Centre of Infectious and Parasitic DiseasesAgrobioinstitute, Agricultural AcademyAgrobioinstitute, Agricultural AcademyNational Centre of Infectious and Parasitic DiseasesBulgarian Academy of SciencesBulgarian Academy of SciencesBulgarian Academy of SciencesGraptopetalum paraguayense E. Walther (GP) belongs to the Crassulaceae family and has several health benefits. In the present study we evaluated the cytotoxic, anti-Herpes Simplex virus (HSV) and antibacterial activities of a total methanol/water GP extract. The results from the cytotoxicity investigation of the tested extract showed a high cell tolerable concentration range. The GP extract demonstrated a significant inhibitory effect on the wild-type HSV-1 strain Victoria in a concentration of 0.0001 mg/mL equal to the maximal nontoxic concentration (with 97.5% protection of the cells). Regarding the acyclovir-resistant mutant HSV-2 strain PU and wild-type HSV-2 strain Bja, the antiviral activity and selectivity of the extract were very low, with EC50 values from 0.1 to 0.01 mg/mL, and the selectivity index ranging from 25 to 250, respectively. The GP extract exhibited selective inhibitory activity also on the growth of Gram-positive bacterial pathogens, with Staphylococcus aureus being most sensitive. It also prevented the formation of MRSA biofilm up to 50% at a concentration of 1.6 mg/mL and up to 90% at a concentration of 2.5 mg/mL. The anti-herpetic and antibacterial activities demonstrated in our investigation are probably related to the presence of phenols, and in particular gallic acid. The antimicrobial activity of the GP extract holds a promise for the future development of nutrition supplements based on G. paraguayense and therefore warrants further studies aimed at isolating biologically active compounds or mixtures thereof with subsequent in-depth investigation of their anti-HSV and antibacterial mechanisms of action.http://dx.doi.org/10.1080/13102818.2019.1656108graptopetalum paraguayense e. walther extractanti-hsv activities anti-bacterial effectminimal inhibitory concentrationeffective concentration 50% (ec50)cytotoxicity |
| spellingShingle | Maya Margaritova Zaharieva Petia Genova-Kalоu Ivayla Dincheva Ilian Badjakov Stefka Krumova Venelin Enchev Hristo Najdenski Nadezhda Markova Anti-Herpes Simplex virus and antibacterial activities of Graptopetalum paraguayense E. Walther leaf extract: a pilot study Biotechnology & Biotechnological Equipment graptopetalum paraguayense e. walther extract anti-hsv activities anti-bacterial effect minimal inhibitory concentration effective concentration 50% (ec50) cytotoxicity |
| title | Anti-Herpes Simplex virus and antibacterial activities of Graptopetalum paraguayense E. Walther leaf extract: a pilot study |
| title_full | Anti-Herpes Simplex virus and antibacterial activities of Graptopetalum paraguayense E. Walther leaf extract: a pilot study |
| title_fullStr | Anti-Herpes Simplex virus and antibacterial activities of Graptopetalum paraguayense E. Walther leaf extract: a pilot study |
| title_full_unstemmed | Anti-Herpes Simplex virus and antibacterial activities of Graptopetalum paraguayense E. Walther leaf extract: a pilot study |
| title_short | Anti-Herpes Simplex virus and antibacterial activities of Graptopetalum paraguayense E. Walther leaf extract: a pilot study |
| title_sort | anti herpes simplex virus and antibacterial activities of graptopetalum paraguayense e walther leaf extract a pilot study |
| topic | graptopetalum paraguayense e. walther extract anti-hsv activities anti-bacterial effect minimal inhibitory concentration effective concentration 50% (ec50) cytotoxicity |
| url | http://dx.doi.org/10.1080/13102818.2019.1656108 |
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