An Outer Membrane Vesicle-Adjuvanted Oral Vaccine Protects Against Lethal, Oral <i>Salmonella</i> Infection
Non-typhoidal salmonellosis, caused by <i>Salmonella enterica</i> serovar Typhimurium is a common fecal-oral disease characterized by mild gastrointestinal distress resulting in diarrhea, chills, fever, abdominal cramps, head and body aches, nausea, and vomiting. Increasing incidences of...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/5/616 |
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| author | Jaikin E. Harrell Jonathan R. Kurtz David L. Bauer J. Timothy Prior Patrick S. Gellings Lisa A. Morici James B. McLachlan |
| author_facet | Jaikin E. Harrell Jonathan R. Kurtz David L. Bauer J. Timothy Prior Patrick S. Gellings Lisa A. Morici James B. McLachlan |
| author_sort | Jaikin E. Harrell |
| collection | DOAJ |
| description | Non-typhoidal salmonellosis, caused by <i>Salmonella enterica</i> serovar Typhimurium is a common fecal-oral disease characterized by mild gastrointestinal distress resulting in diarrhea, chills, fever, abdominal cramps, head and body aches, nausea, and vomiting. Increasing incidences of antibiotic resistant invasive non-typhoidal <i>Salmonella</i> infections makes this a global threat requiring novel treatment strategies including next-generation vaccines. The goal of the current study was to formulate a novel vaccine platform against <i>Salmonella</i> infection that could be delivered orally. To accomplish this, we created a <i>Salmonella</i>-specific vaccine adjuvanted with <i>Burkholderia pseudomallei</i> outer membrane vesicles (OMVs). We show that adding OMVs to a heat-killed oral <i>Salmonella</i> vaccine (HKST + OMVs) protects against a lethal, oral challenge with <i>Salmonella</i>. Further, we show that opsonizing anti-<i>Salmonella</i> antibodies are induced in response to immunization and that CD4 T cells and B cells can be induced when OMVs are used as the oral adjuvant. This study represents a novel oral vaccine approach to combatting the increasing problem of invasive <i>Salmonella</i> infections. |
| first_indexed | 2024-03-10T11:19:04Z |
| format | Article |
| id | doaj.art-6aef9d7d4536401c8b64aa1b108a6708 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T11:19:04Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-6aef9d7d4536401c8b64aa1b108a67082023-11-21T20:10:22ZengMDPI AGPathogens2076-08172021-05-0110561610.3390/pathogens10050616An Outer Membrane Vesicle-Adjuvanted Oral Vaccine Protects Against Lethal, Oral <i>Salmonella</i> InfectionJaikin E. Harrell0Jonathan R. Kurtz1David L. Bauer2J. Timothy Prior3Patrick S. Gellings4Lisa A. Morici5James B. McLachlan6Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA 70112, USADepartment of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA 70112, USADepartment of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA 70112, USADepartment of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA 70112, USADepartment of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA 70112, USADepartment of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA 70112, USADepartment of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA 70112, USANon-typhoidal salmonellosis, caused by <i>Salmonella enterica</i> serovar Typhimurium is a common fecal-oral disease characterized by mild gastrointestinal distress resulting in diarrhea, chills, fever, abdominal cramps, head and body aches, nausea, and vomiting. Increasing incidences of antibiotic resistant invasive non-typhoidal <i>Salmonella</i> infections makes this a global threat requiring novel treatment strategies including next-generation vaccines. The goal of the current study was to formulate a novel vaccine platform against <i>Salmonella</i> infection that could be delivered orally. To accomplish this, we created a <i>Salmonella</i>-specific vaccine adjuvanted with <i>Burkholderia pseudomallei</i> outer membrane vesicles (OMVs). We show that adding OMVs to a heat-killed oral <i>Salmonella</i> vaccine (HKST + OMVs) protects against a lethal, oral challenge with <i>Salmonella</i>. Further, we show that opsonizing anti-<i>Salmonella</i> antibodies are induced in response to immunization and that CD4 T cells and B cells can be induced when OMVs are used as the oral adjuvant. This study represents a novel oral vaccine approach to combatting the increasing problem of invasive <i>Salmonella</i> infections.https://www.mdpi.com/2076-0817/10/5/616<i>Salmonella</i>mucosal immunityouter membrane vesiclesvaccine |
| spellingShingle | Jaikin E. Harrell Jonathan R. Kurtz David L. Bauer J. Timothy Prior Patrick S. Gellings Lisa A. Morici James B. McLachlan An Outer Membrane Vesicle-Adjuvanted Oral Vaccine Protects Against Lethal, Oral <i>Salmonella</i> Infection Pathogens <i>Salmonella</i> mucosal immunity outer membrane vesicles vaccine |
| title | An Outer Membrane Vesicle-Adjuvanted Oral Vaccine Protects Against Lethal, Oral <i>Salmonella</i> Infection |
| title_full | An Outer Membrane Vesicle-Adjuvanted Oral Vaccine Protects Against Lethal, Oral <i>Salmonella</i> Infection |
| title_fullStr | An Outer Membrane Vesicle-Adjuvanted Oral Vaccine Protects Against Lethal, Oral <i>Salmonella</i> Infection |
| title_full_unstemmed | An Outer Membrane Vesicle-Adjuvanted Oral Vaccine Protects Against Lethal, Oral <i>Salmonella</i> Infection |
| title_short | An Outer Membrane Vesicle-Adjuvanted Oral Vaccine Protects Against Lethal, Oral <i>Salmonella</i> Infection |
| title_sort | outer membrane vesicle adjuvanted oral vaccine protects against lethal oral i salmonella i infection |
| topic | <i>Salmonella</i> mucosal immunity outer membrane vesicles vaccine |
| url | https://www.mdpi.com/2076-0817/10/5/616 |
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