The Dose-Related Efficacy of Human Placenta-Derived Mesenchymal Stem Cell Transplantation on Antioxidant Effects in a Rat Model with Ovariectomy
Oxidative stress initiates various degenerative diseases, and it is caused by excessive reactive oxygen species (ROS) production. Oxidative stress is a key factor that causes infertility by inducing ovarian dysfunction, characterized by irregular hormone levels, lower quality of mature follicles, an...
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MDPI AG
2023-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/8/1575 |
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| author | Jin Seok Hyeri Park Dae-Hyun Lee Jun Hyeong You Gi Jin Kim |
| author_facet | Jin Seok Hyeri Park Dae-Hyun Lee Jun Hyeong You Gi Jin Kim |
| author_sort | Jin Seok |
| collection | DOAJ |
| description | Oxidative stress initiates various degenerative diseases, and it is caused by excessive reactive oxygen species (ROS) production. Oxidative stress is a key factor that causes infertility by inducing ovarian dysfunction, characterized by irregular hormone levels, lower quality of mature follicles, and loss of follicles. Hence, stem cell therapy has been actively studied as an approach to overcome the side effects of hormone replacement therapy (HRT) on ovarian dysfunction. However, there is a lack of evidence about the appropriate number of cells required for stem cell therapy. Therefore, based on the antioxidant effects investigated in this study, we focused on determining the appropriate dose of stem cells for transplantation in an animal model with ovarian dysfunction. One week after half-ovariectomy, placenta-derived mesenchymal stem cells (PD-MSCs, 1 × 10<sup>5</sup> cells, 5 × 10<sup>5</sup> cells, or 2.5 × 10<sup>6</sup> cells) were injected intravenously into the Tx groups through the tail vein. As a result, the mRNA expression of hAlu gradually increased as the transplanted cell concentration increased. Compared with no transplantation (NTx), the transplantation of PD-MSCs improved folliculogenesis, including the levels of secreted hormones and numbers of follicles, by exerting antioxidant effects. Also, the levels of oxidized glutathione in the serum of animal models after transplantation were significantly increased (* <i>p</i> < 0.05). These results indicated that PD-MSC transplantation improved ovarian function in half-ovariectomized rats by exerting antioxidant effects. According to our data, increasing the number of transplanted cells did not proportionally increase the effectiveness of the treatment. We suggest that low-dose PD-MSC transplantation has the same therapeutic effect as described in previous studies. These findings provide new insights for further understanding reproductive systems and provide evidence for related clinical trials. |
| first_indexed | 2024-03-11T00:10:33Z |
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| last_indexed | 2024-03-11T00:10:33Z |
| publishDate | 2023-08-01 |
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| series | Antioxidants |
| spelling | doaj.art-6af036a0d00545cb83f790cb266754a32023-11-19T00:02:07ZengMDPI AGAntioxidants2076-39212023-08-01128157510.3390/antiox12081575The Dose-Related Efficacy of Human Placenta-Derived Mesenchymal Stem Cell Transplantation on Antioxidant Effects in a Rat Model with OvariectomyJin Seok0Hyeri Park1Dae-Hyun Lee2Jun Hyeong You3Gi Jin Kim4Department of Bioinspired Science, CHA University, Seongnam-si 13488, Republic of KoreaDepartment of Bioinspired Science, CHA University, Seongnam-si 13488, Republic of KoreaDepartment of Bioinspired Science, CHA University, Seongnam-si 13488, Republic of KoreaDepartment of Bioinspired Science, CHA University, Seongnam-si 13488, Republic of KoreaDepartment of Bioinspired Science, CHA University, Seongnam-si 13488, Republic of KoreaOxidative stress initiates various degenerative diseases, and it is caused by excessive reactive oxygen species (ROS) production. Oxidative stress is a key factor that causes infertility by inducing ovarian dysfunction, characterized by irregular hormone levels, lower quality of mature follicles, and loss of follicles. Hence, stem cell therapy has been actively studied as an approach to overcome the side effects of hormone replacement therapy (HRT) on ovarian dysfunction. However, there is a lack of evidence about the appropriate number of cells required for stem cell therapy. Therefore, based on the antioxidant effects investigated in this study, we focused on determining the appropriate dose of stem cells for transplantation in an animal model with ovarian dysfunction. One week after half-ovariectomy, placenta-derived mesenchymal stem cells (PD-MSCs, 1 × 10<sup>5</sup> cells, 5 × 10<sup>5</sup> cells, or 2.5 × 10<sup>6</sup> cells) were injected intravenously into the Tx groups through the tail vein. As a result, the mRNA expression of hAlu gradually increased as the transplanted cell concentration increased. Compared with no transplantation (NTx), the transplantation of PD-MSCs improved folliculogenesis, including the levels of secreted hormones and numbers of follicles, by exerting antioxidant effects. Also, the levels of oxidized glutathione in the serum of animal models after transplantation were significantly increased (* <i>p</i> < 0.05). These results indicated that PD-MSC transplantation improved ovarian function in half-ovariectomized rats by exerting antioxidant effects. According to our data, increasing the number of transplanted cells did not proportionally increase the effectiveness of the treatment. We suggest that low-dose PD-MSC transplantation has the same therapeutic effect as described in previous studies. These findings provide new insights for further understanding reproductive systems and provide evidence for related clinical trials.https://www.mdpi.com/2076-3921/12/8/1575ovariectomized rat modelplacenta-derived mesenchymal stem cellsovaryfolliculogenesisantioxidants |
| spellingShingle | Jin Seok Hyeri Park Dae-Hyun Lee Jun Hyeong You Gi Jin Kim The Dose-Related Efficacy of Human Placenta-Derived Mesenchymal Stem Cell Transplantation on Antioxidant Effects in a Rat Model with Ovariectomy Antioxidants ovariectomized rat model placenta-derived mesenchymal stem cells ovary folliculogenesis antioxidants |
| title | The Dose-Related Efficacy of Human Placenta-Derived Mesenchymal Stem Cell Transplantation on Antioxidant Effects in a Rat Model with Ovariectomy |
| title_full | The Dose-Related Efficacy of Human Placenta-Derived Mesenchymal Stem Cell Transplantation on Antioxidant Effects in a Rat Model with Ovariectomy |
| title_fullStr | The Dose-Related Efficacy of Human Placenta-Derived Mesenchymal Stem Cell Transplantation on Antioxidant Effects in a Rat Model with Ovariectomy |
| title_full_unstemmed | The Dose-Related Efficacy of Human Placenta-Derived Mesenchymal Stem Cell Transplantation on Antioxidant Effects in a Rat Model with Ovariectomy |
| title_short | The Dose-Related Efficacy of Human Placenta-Derived Mesenchymal Stem Cell Transplantation on Antioxidant Effects in a Rat Model with Ovariectomy |
| title_sort | dose related efficacy of human placenta derived mesenchymal stem cell transplantation on antioxidant effects in a rat model with ovariectomy |
| topic | ovariectomized rat model placenta-derived mesenchymal stem cells ovary folliculogenesis antioxidants |
| url | https://www.mdpi.com/2076-3921/12/8/1575 |
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