Models for Gut-Mediated Horizontal Gene Transfer by Bacterial Plasmid Conjugation
The emergence of new antimicrobial resistant and virulent bacterial strains may pose a threat to human and animal health. Bacterial plasmid conjugation is a significant contributor to rapid microbial evolutions that results in the emergence and spread of antimicrobial resistance (AR). The gut of ani...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.891548/full |
| _version_ | 1818549369603883008 |
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| author | Logan C. Ott Logan C. Ott Melha Mellata Melha Mellata |
| author_facet | Logan C. Ott Logan C. Ott Melha Mellata Melha Mellata |
| author_sort | Logan C. Ott |
| collection | DOAJ |
| description | The emergence of new antimicrobial resistant and virulent bacterial strains may pose a threat to human and animal health. Bacterial plasmid conjugation is a significant contributor to rapid microbial evolutions that results in the emergence and spread of antimicrobial resistance (AR). The gut of animals is believed to be a potent reservoir for the spread of AR and virulence genes through the horizontal exchange of mobile genetic elements such as plasmids. The study of the plasmid transfer process in the complex gut environment is limited due to the confounding factors that affect colonization, persistence, and plasmid conjugation. Furthermore, study of plasmid transfer in the gut of humans is limited to observational studies, leading to the need to identify alternate models that provide insight into the factors regulating conjugation in the gut. This review discusses key studies on the current models for in silico, in vitro, and in vivo modeling of bacterial conjugation, and their ability to reflect the gut of animals. We particularly emphasize the use of computational and in vitro models that may approximate aspects of the gut, as well as animal models that represent in vivo conditions to a greater extent. Directions on future research studies in the field are provided. |
| first_indexed | 2024-12-12T08:32:27Z |
| format | Article |
| id | doaj.art-6af268e8febd4d73b0f3c63436ce1469 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T08:32:27Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6af268e8febd4d73b0f3c63436ce14692022-12-22T00:31:04ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-06-011310.3389/fmicb.2022.891548891548Models for Gut-Mediated Horizontal Gene Transfer by Bacterial Plasmid ConjugationLogan C. Ott0Logan C. Ott1Melha Mellata2Melha Mellata3Department of Food Science and Human Nutrition, Iowa State University, Ames, IA, United StatesInterdepartmental Microbiology Graduate Program, Iowa State University, Ames, IA, United StatesDepartment of Food Science and Human Nutrition, Iowa State University, Ames, IA, United StatesInterdepartmental Microbiology Graduate Program, Iowa State University, Ames, IA, United StatesThe emergence of new antimicrobial resistant and virulent bacterial strains may pose a threat to human and animal health. Bacterial plasmid conjugation is a significant contributor to rapid microbial evolutions that results in the emergence and spread of antimicrobial resistance (AR). The gut of animals is believed to be a potent reservoir for the spread of AR and virulence genes through the horizontal exchange of mobile genetic elements such as plasmids. The study of the plasmid transfer process in the complex gut environment is limited due to the confounding factors that affect colonization, persistence, and plasmid conjugation. Furthermore, study of plasmid transfer in the gut of humans is limited to observational studies, leading to the need to identify alternate models that provide insight into the factors regulating conjugation in the gut. This review discusses key studies on the current models for in silico, in vitro, and in vivo modeling of bacterial conjugation, and their ability to reflect the gut of animals. We particularly emphasize the use of computational and in vitro models that may approximate aspects of the gut, as well as animal models that represent in vivo conditions to a greater extent. Directions on future research studies in the field are provided.https://www.frontiersin.org/articles/10.3389/fmicb.2022.891548/fullhorizontal gene transferplasmidsbacterial evolutionin vivoin vitroantimicrobial resistance |
| spellingShingle | Logan C. Ott Logan C. Ott Melha Mellata Melha Mellata Models for Gut-Mediated Horizontal Gene Transfer by Bacterial Plasmid Conjugation Frontiers in Microbiology horizontal gene transfer plasmids bacterial evolution in vivo in vitro antimicrobial resistance |
| title | Models for Gut-Mediated Horizontal Gene Transfer by Bacterial Plasmid Conjugation |
| title_full | Models for Gut-Mediated Horizontal Gene Transfer by Bacterial Plasmid Conjugation |
| title_fullStr | Models for Gut-Mediated Horizontal Gene Transfer by Bacterial Plasmid Conjugation |
| title_full_unstemmed | Models for Gut-Mediated Horizontal Gene Transfer by Bacterial Plasmid Conjugation |
| title_short | Models for Gut-Mediated Horizontal Gene Transfer by Bacterial Plasmid Conjugation |
| title_sort | models for gut mediated horizontal gene transfer by bacterial plasmid conjugation |
| topic | horizontal gene transfer plasmids bacterial evolution in vivo in vitro antimicrobial resistance |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.891548/full |
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