MicroRNA-96-5p is negatively regulating GPC3 in the metastasis of papillary thyroid cancer
Backgrounds: Papillary thyroid cancer is the most common pathological type of thyroid cancer. miR-96-5p, a member of the miR-183 family, constitute a polycistronic miRNA cluster. In breast cancer, miR-96-5p promotes cell invasion, migration, and proliferation in vitro by inhibiting PTPN9. Moreover,...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2023-10-01
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| Series: | SAGE Open Medicine |
| Online Access: | https://doi.org/10.1177/20503121231205710 |
| _version_ | 1827778452115161088 |
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| author | Haibei Hu Guangqian Quan Feng Yang Shan Du Siqin Ding Yongzhi Lun Qiang Chen |
| author_facet | Haibei Hu Guangqian Quan Feng Yang Shan Du Siqin Ding Yongzhi Lun Qiang Chen |
| author_sort | Haibei Hu |
| collection | DOAJ |
| description | Backgrounds: Papillary thyroid cancer is the most common pathological type of thyroid cancer. miR-96-5p, a member of the miR-183 family, constitute a polycistronic miRNA cluster. In breast cancer, miR-96-5p promotes cell invasion, migration, and proliferation in vitro by inhibiting PTPN9. Moreover, miR-96-5p was reported to function as an oncogene in many cancers. However, whether miR-96-5p is involved in the development of papillary thyroid cancers and its potential mechanism is still unknown. The present study aims to explore the relationship between miR-96-5p and GPC3 expression in the development of papillary thyroid cancers. Methods: Transcriptomic sequencing was carried out using six pairs of papillary thyroid cancer and adjacent normal tissues. Quantitative real-time polymerase chain reaction (PCR) experiments were performed to examine the expression of genes. Results: In total, there were 1588 up-regulated and 1803 down-regulated differentially expressed genes between papillary thyroid cancer and normal tissues. Gene ontology and Kyoto encyclopedia of genes and genomes analysis revealed that extracellular matrix structure and proteoglycans were mainly involved in papillary thyroid cancer. Among the cluster of proteoglycans, GPC3 was significantly down-regulated in papillary thyroid cancer and is a target of miR-96. Conclusion: miR-96-5p participates in the development of papillary thyroid cancer by regulating the expression of GPC3. Thus, targeting miR-96-5p may be a potential therapeutic approach for preventing and treating papillary thyroid cancer. |
| first_indexed | 2024-03-11T14:34:18Z |
| format | Article |
| id | doaj.art-6af3bb15ff56416c9b0f10c8bd8ffefd |
| institution | Directory Open Access Journal |
| issn | 2050-3121 |
| language | English |
| last_indexed | 2024-03-11T14:34:18Z |
| publishDate | 2023-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | SAGE Open Medicine |
| spelling | doaj.art-6af3bb15ff56416c9b0f10c8bd8ffefd2023-10-31T05:35:58ZengSAGE PublishingSAGE Open Medicine2050-31212023-10-011110.1177/20503121231205710MicroRNA-96-5p is negatively regulating GPC3 in the metastasis of papillary thyroid cancerHaibei Hu0Guangqian Quan1Feng Yang2Shan Du3Siqin Ding4Yongzhi Lun5Qiang Chen6Key Laboratory of Medical Microecology, Fujian Province University, School of Pharmacy and Medical Technology, Putian University, Putian, Fujian, ChinaDepartment of Breast Surgery, Nanping First Hospital, Fujian Medical University, Nanping, Fujian, ChinaDepartment of General Surgery, The Third People’s Hospital of Fujian Province, Fuzhou, Fujian, ChinaDepartment of Pathology, Shenzhen Hospital (Guangming), University of Chinese Academy of Sciences, Shenzhen, Guangdong, ChinaDepartment of Thyroid and Breast Surgery, Shenzhen Hospital (Guangming), University of Chinese Academy of Sciences, Shenzhen, Guangdong, ChinaKey Laboratory of Medical Microecology, Fujian Province University, School of Pharmacy and Medical Technology, Putian University, Putian, Fujian, ChinaDepartment of Thyroid and Breast Surgery, Shenzhen Hospital (Guangming), University of Chinese Academy of Sciences, Shenzhen, Guangdong, ChinaBackgrounds: Papillary thyroid cancer is the most common pathological type of thyroid cancer. miR-96-5p, a member of the miR-183 family, constitute a polycistronic miRNA cluster. In breast cancer, miR-96-5p promotes cell invasion, migration, and proliferation in vitro by inhibiting PTPN9. Moreover, miR-96-5p was reported to function as an oncogene in many cancers. However, whether miR-96-5p is involved in the development of papillary thyroid cancers and its potential mechanism is still unknown. The present study aims to explore the relationship between miR-96-5p and GPC3 expression in the development of papillary thyroid cancers. Methods: Transcriptomic sequencing was carried out using six pairs of papillary thyroid cancer and adjacent normal tissues. Quantitative real-time polymerase chain reaction (PCR) experiments were performed to examine the expression of genes. Results: In total, there were 1588 up-regulated and 1803 down-regulated differentially expressed genes between papillary thyroid cancer and normal tissues. Gene ontology and Kyoto encyclopedia of genes and genomes analysis revealed that extracellular matrix structure and proteoglycans were mainly involved in papillary thyroid cancer. Among the cluster of proteoglycans, GPC3 was significantly down-regulated in papillary thyroid cancer and is a target of miR-96. Conclusion: miR-96-5p participates in the development of papillary thyroid cancer by regulating the expression of GPC3. Thus, targeting miR-96-5p may be a potential therapeutic approach for preventing and treating papillary thyroid cancer.https://doi.org/10.1177/20503121231205710 |
| spellingShingle | Haibei Hu Guangqian Quan Feng Yang Shan Du Siqin Ding Yongzhi Lun Qiang Chen MicroRNA-96-5p is negatively regulating GPC3 in the metastasis of papillary thyroid cancer SAGE Open Medicine |
| title | MicroRNA-96-5p is negatively regulating GPC3 in the metastasis of papillary thyroid cancer |
| title_full | MicroRNA-96-5p is negatively regulating GPC3 in the metastasis of papillary thyroid cancer |
| title_fullStr | MicroRNA-96-5p is negatively regulating GPC3 in the metastasis of papillary thyroid cancer |
| title_full_unstemmed | MicroRNA-96-5p is negatively regulating GPC3 in the metastasis of papillary thyroid cancer |
| title_short | MicroRNA-96-5p is negatively regulating GPC3 in the metastasis of papillary thyroid cancer |
| title_sort | microrna 96 5p is negatively regulating gpc3 in the metastasis of papillary thyroid cancer |
| url | https://doi.org/10.1177/20503121231205710 |
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