Transcriptome profiling of a <it>Sinorhizobium meliloti fadD </it>mutant reveals the role of rhizobactin 1021 biosynthesis and regulation genes in the control of swarming
<p>Abstract</p> <p>Background</p> <p>Swarming is a multicellular phenomenom characterized by the coordinated and rapid movement of bacteria across semisolid surfaces. In <it>Sinorhizobium meliloti </it>this type of motility has been described in a <it>...
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| Format: | Article |
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BMC
2010-03-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/11/157 |
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| author | Olivares José Sanjuán Juan Cuéllar Virginia van Dillewijn Pieter Amaya-Gómez Carol V Domínguez-Ferreras Ana Nogales Joaquina Soto María J |
| author_facet | Olivares José Sanjuán Juan Cuéllar Virginia van Dillewijn Pieter Amaya-Gómez Carol V Domínguez-Ferreras Ana Nogales Joaquina Soto María J |
| author_sort | Olivares José |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Swarming is a multicellular phenomenom characterized by the coordinated and rapid movement of bacteria across semisolid surfaces. In <it>Sinorhizobium meliloti </it>this type of motility has been described in a <it>fadD </it>mutant. To gain insights into the mechanisms underlying the process of swarming in rhizobia, we compared the transcriptome of a <it>S. meliloti fadD </it>mutant grown under swarming inducing conditions (semisolid medium) to those of cells grown under non-swarming conditions (broth and solid medium).</p> <p>Results</p> <p>More than a thousand genes were identified as differentially expressed in response to growth on agar surfaces including genes for several metabolic activities, iron uptake, chemotaxis, motility and stress-related genes. Under swarming-specific conditions, the most remarkable response was the up-regulation of iron-related genes. We demonstrate that the pSymA plasmid and specifically genes required for the biosynthesis of the siderophore rhizobactin 1021 are essential for swarming of a <it>S. meliloti </it>wild-type strain but not in a <it>fadD </it>mutant. Moreover, high iron conditions inhibit swarming of the wild-type strain but not in mutants lacking either the iron limitation response regulator RirA or FadD.</p> <p>Conclusions</p> <p>The present work represents the first transcriptomic study of rhizobium growth on surfaces including swarming inducing conditions. The results have revealed major changes in the physiology of <it>S. meliloti </it>cells grown on a surface relative to liquid cultures. Moreover, analysis of genes responding to swarming inducing conditions led to the demonstration that iron and genes involved in rhizobactin 1021 synthesis play a role in the surface motility shown by <it>S. meliloti </it>which can be circumvented in a <it>fadD </it>mutant. This work opens a way to the identification of new traits and regulatory networks involved in swarming by rhizobia.</p> |
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| id | doaj.art-6afb1dad8a5f4c1eb9ee725f083be795 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-13T02:23:03Z |
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| spelling | doaj.art-6afb1dad8a5f4c1eb9ee725f083be7952022-12-22T03:06:53ZengBMCBMC Genomics1471-21642010-03-0111115710.1186/1471-2164-11-157Transcriptome profiling of a <it>Sinorhizobium meliloti fadD </it>mutant reveals the role of rhizobactin 1021 biosynthesis and regulation genes in the control of swarmingOlivares JoséSanjuán JuanCuéllar Virginiavan Dillewijn PieterAmaya-Gómez Carol VDomínguez-Ferreras AnaNogales JoaquinaSoto María J<p>Abstract</p> <p>Background</p> <p>Swarming is a multicellular phenomenom characterized by the coordinated and rapid movement of bacteria across semisolid surfaces. In <it>Sinorhizobium meliloti </it>this type of motility has been described in a <it>fadD </it>mutant. To gain insights into the mechanisms underlying the process of swarming in rhizobia, we compared the transcriptome of a <it>S. meliloti fadD </it>mutant grown under swarming inducing conditions (semisolid medium) to those of cells grown under non-swarming conditions (broth and solid medium).</p> <p>Results</p> <p>More than a thousand genes were identified as differentially expressed in response to growth on agar surfaces including genes for several metabolic activities, iron uptake, chemotaxis, motility and stress-related genes. Under swarming-specific conditions, the most remarkable response was the up-regulation of iron-related genes. We demonstrate that the pSymA plasmid and specifically genes required for the biosynthesis of the siderophore rhizobactin 1021 are essential for swarming of a <it>S. meliloti </it>wild-type strain but not in a <it>fadD </it>mutant. Moreover, high iron conditions inhibit swarming of the wild-type strain but not in mutants lacking either the iron limitation response regulator RirA or FadD.</p> <p>Conclusions</p> <p>The present work represents the first transcriptomic study of rhizobium growth on surfaces including swarming inducing conditions. The results have revealed major changes in the physiology of <it>S. meliloti </it>cells grown on a surface relative to liquid cultures. Moreover, analysis of genes responding to swarming inducing conditions led to the demonstration that iron and genes involved in rhizobactin 1021 synthesis play a role in the surface motility shown by <it>S. meliloti </it>which can be circumvented in a <it>fadD </it>mutant. This work opens a way to the identification of new traits and regulatory networks involved in swarming by rhizobia.</p>http://www.biomedcentral.com/1471-2164/11/157 |
| spellingShingle | Olivares José Sanjuán Juan Cuéllar Virginia van Dillewijn Pieter Amaya-Gómez Carol V Domínguez-Ferreras Ana Nogales Joaquina Soto María J Transcriptome profiling of a <it>Sinorhizobium meliloti fadD </it>mutant reveals the role of rhizobactin 1021 biosynthesis and regulation genes in the control of swarming BMC Genomics |
| title | Transcriptome profiling of a <it>Sinorhizobium meliloti fadD </it>mutant reveals the role of rhizobactin 1021 biosynthesis and regulation genes in the control of swarming |
| title_full | Transcriptome profiling of a <it>Sinorhizobium meliloti fadD </it>mutant reveals the role of rhizobactin 1021 biosynthesis and regulation genes in the control of swarming |
| title_fullStr | Transcriptome profiling of a <it>Sinorhizobium meliloti fadD </it>mutant reveals the role of rhizobactin 1021 biosynthesis and regulation genes in the control of swarming |
| title_full_unstemmed | Transcriptome profiling of a <it>Sinorhizobium meliloti fadD </it>mutant reveals the role of rhizobactin 1021 biosynthesis and regulation genes in the control of swarming |
| title_short | Transcriptome profiling of a <it>Sinorhizobium meliloti fadD </it>mutant reveals the role of rhizobactin 1021 biosynthesis and regulation genes in the control of swarming |
| title_sort | transcriptome profiling of a it sinorhizobium meliloti fadd it mutant reveals the role of rhizobactin 1021 biosynthesis and regulation genes in the control of swarming |
| url | http://www.biomedcentral.com/1471-2164/11/157 |
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