Cellular Effects of Cyclodextrins: Studies on HeLa Cells

Cyclodextrins are high molecular weight, hydrophilic, cyclic, non-reducing oligosaccharides, applied as excipients for the improvement of the solubility and permeability of insoluble active pharmaceutical ingredients. On the other hand, beta-cyclodextrins are used as cholesterol sequestering agents...

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Main Authors: Ágnes Rusznyák, Mercédesz Palicskó, Milo Malanga, Éva Fenyvesi, Lajos Szente, Judit Váradi, Ildikó Bácskay, Miklós Vecsernyés, Katalin Szászné Réti-Nagy, Gábor Vasvári, Ádám Haimhoffer, Ferenc Fenyvesi
Format: Article
Language:English
Published: MDPI AG 2022-02-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/27/5/1589
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author Ágnes Rusznyák
Mercédesz Palicskó
Milo Malanga
Éva Fenyvesi
Lajos Szente
Judit Váradi
Ildikó Bácskay
Miklós Vecsernyés
Katalin Szászné Réti-Nagy
Gábor Vasvári
Ádám Haimhoffer
Ferenc Fenyvesi
author_facet Ágnes Rusznyák
Mercédesz Palicskó
Milo Malanga
Éva Fenyvesi
Lajos Szente
Judit Váradi
Ildikó Bácskay
Miklós Vecsernyés
Katalin Szászné Réti-Nagy
Gábor Vasvári
Ádám Haimhoffer
Ferenc Fenyvesi
author_sort Ágnes Rusznyák
collection DOAJ
description Cyclodextrins are high molecular weight, hydrophilic, cyclic, non-reducing oligosaccharides, applied as excipients for the improvement of the solubility and permeability of insoluble active pharmaceutical ingredients. On the other hand, beta-cyclodextrins are used as cholesterol sequestering agents in life sciences. Recently, we demonstrated the cellular internalization and intracellular effects of cyclodextrins on Caco-2 cells. In this study, we aimed to further investigate the endocytosis of (2-hydroxylpropyl)-beta-(HPBCD) and random methylated-beta-cyclodextrin (RAMEB) to test their cytotoxicity, NF-kappa B pathway induction, autophagy, and lysosome formation on HeLa cells. These derivatives were able to enter the cells; however, major differences were revealed in the inhibition of their endocytosis compared to Caco-2 cells. NF-kappa B p65 translocation was not detected in the cell nuclei after HPBCD or RAMEB pre-treatment and cyclodextrin treatment did not enhance the formation of autophagosomes. These cyclodextrin derivates were partially localized in lysosomes after internalization.
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spelling doaj.art-6afc9334d1834ab9bf4988ba85e5c5252023-11-23T23:26:45ZengMDPI AGMolecules1420-30492022-02-01275158910.3390/molecules27051589Cellular Effects of Cyclodextrins: Studies on HeLa CellsÁgnes Rusznyák0Mercédesz Palicskó1Milo Malanga2Éva Fenyvesi3Lajos Szente4Judit Váradi5Ildikó Bácskay6Miklós Vecsernyés7Katalin Szászné Réti-Nagy8Gábor Vasvári9Ádám Haimhoffer10Ferenc Fenyvesi11Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, HungaryCycloLab Cyclodextrin R&D Laboratory Ltd., Illatos St. 7, H-1097 Budapest, HungaryCycloLab Cyclodextrin R&D Laboratory Ltd., Illatos St. 7, H-1097 Budapest, HungaryCycloLab Cyclodextrin R&D Laboratory Ltd., Illatos St. 7, H-1097 Budapest, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, HungaryCyclodextrins are high molecular weight, hydrophilic, cyclic, non-reducing oligosaccharides, applied as excipients for the improvement of the solubility and permeability of insoluble active pharmaceutical ingredients. On the other hand, beta-cyclodextrins are used as cholesterol sequestering agents in life sciences. Recently, we demonstrated the cellular internalization and intracellular effects of cyclodextrins on Caco-2 cells. In this study, we aimed to further investigate the endocytosis of (2-hydroxylpropyl)-beta-(HPBCD) and random methylated-beta-cyclodextrin (RAMEB) to test their cytotoxicity, NF-kappa B pathway induction, autophagy, and lysosome formation on HeLa cells. These derivatives were able to enter the cells; however, major differences were revealed in the inhibition of their endocytosis compared to Caco-2 cells. NF-kappa B p65 translocation was not detected in the cell nuclei after HPBCD or RAMEB pre-treatment and cyclodextrin treatment did not enhance the formation of autophagosomes. These cyclodextrin derivates were partially localized in lysosomes after internalization.https://www.mdpi.com/1420-3049/27/5/1589HPBCDRAMEBendocytosislysosomesautophagyNF-κB
spellingShingle Ágnes Rusznyák
Mercédesz Palicskó
Milo Malanga
Éva Fenyvesi
Lajos Szente
Judit Váradi
Ildikó Bácskay
Miklós Vecsernyés
Katalin Szászné Réti-Nagy
Gábor Vasvári
Ádám Haimhoffer
Ferenc Fenyvesi
Cellular Effects of Cyclodextrins: Studies on HeLa Cells
Molecules
HPBCD
RAMEB
endocytosis
lysosomes
autophagy
NF-κB
title Cellular Effects of Cyclodextrins: Studies on HeLa Cells
title_full Cellular Effects of Cyclodextrins: Studies on HeLa Cells
title_fullStr Cellular Effects of Cyclodextrins: Studies on HeLa Cells
title_full_unstemmed Cellular Effects of Cyclodextrins: Studies on HeLa Cells
title_short Cellular Effects of Cyclodextrins: Studies on HeLa Cells
title_sort cellular effects of cyclodextrins studies on hela cells
topic HPBCD
RAMEB
endocytosis
lysosomes
autophagy
NF-κB
url https://www.mdpi.com/1420-3049/27/5/1589
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