Cellular Effects of Cyclodextrins: Studies on HeLa Cells
Cyclodextrins are high molecular weight, hydrophilic, cyclic, non-reducing oligosaccharides, applied as excipients for the improvement of the solubility and permeability of insoluble active pharmaceutical ingredients. On the other hand, beta-cyclodextrins are used as cholesterol sequestering agents...
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MDPI AG
2022-02-01
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author | Ágnes Rusznyák Mercédesz Palicskó Milo Malanga Éva Fenyvesi Lajos Szente Judit Váradi Ildikó Bácskay Miklós Vecsernyés Katalin Szászné Réti-Nagy Gábor Vasvári Ádám Haimhoffer Ferenc Fenyvesi |
author_facet | Ágnes Rusznyák Mercédesz Palicskó Milo Malanga Éva Fenyvesi Lajos Szente Judit Váradi Ildikó Bácskay Miklós Vecsernyés Katalin Szászné Réti-Nagy Gábor Vasvári Ádám Haimhoffer Ferenc Fenyvesi |
author_sort | Ágnes Rusznyák |
collection | DOAJ |
description | Cyclodextrins are high molecular weight, hydrophilic, cyclic, non-reducing oligosaccharides, applied as excipients for the improvement of the solubility and permeability of insoluble active pharmaceutical ingredients. On the other hand, beta-cyclodextrins are used as cholesterol sequestering agents in life sciences. Recently, we demonstrated the cellular internalization and intracellular effects of cyclodextrins on Caco-2 cells. In this study, we aimed to further investigate the endocytosis of (2-hydroxylpropyl)-beta-(HPBCD) and random methylated-beta-cyclodextrin (RAMEB) to test their cytotoxicity, NF-kappa B pathway induction, autophagy, and lysosome formation on HeLa cells. These derivatives were able to enter the cells; however, major differences were revealed in the inhibition of their endocytosis compared to Caco-2 cells. NF-kappa B p65 translocation was not detected in the cell nuclei after HPBCD or RAMEB pre-treatment and cyclodextrin treatment did not enhance the formation of autophagosomes. These cyclodextrin derivates were partially localized in lysosomes after internalization. |
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issn | 1420-3049 |
language | English |
last_indexed | 2024-03-09T20:29:09Z |
publishDate | 2022-02-01 |
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series | Molecules |
spelling | doaj.art-6afc9334d1834ab9bf4988ba85e5c5252023-11-23T23:26:45ZengMDPI AGMolecules1420-30492022-02-01275158910.3390/molecules27051589Cellular Effects of Cyclodextrins: Studies on HeLa CellsÁgnes Rusznyák0Mercédesz Palicskó1Milo Malanga2Éva Fenyvesi3Lajos Szente4Judit Váradi5Ildikó Bácskay6Miklós Vecsernyés7Katalin Szászné Réti-Nagy8Gábor Vasvári9Ádám Haimhoffer10Ferenc Fenyvesi11Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, HungaryCycloLab Cyclodextrin R&D Laboratory Ltd., Illatos St. 7, H-1097 Budapest, HungaryCycloLab Cyclodextrin R&D Laboratory Ltd., Illatos St. 7, H-1097 Budapest, HungaryCycloLab Cyclodextrin R&D Laboratory Ltd., Illatos St. 7, H-1097 Budapest, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, HungaryDepartment of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei St. 98, H-4032 Debrecen, HungaryCyclodextrins are high molecular weight, hydrophilic, cyclic, non-reducing oligosaccharides, applied as excipients for the improvement of the solubility and permeability of insoluble active pharmaceutical ingredients. On the other hand, beta-cyclodextrins are used as cholesterol sequestering agents in life sciences. Recently, we demonstrated the cellular internalization and intracellular effects of cyclodextrins on Caco-2 cells. In this study, we aimed to further investigate the endocytosis of (2-hydroxylpropyl)-beta-(HPBCD) and random methylated-beta-cyclodextrin (RAMEB) to test their cytotoxicity, NF-kappa B pathway induction, autophagy, and lysosome formation on HeLa cells. These derivatives were able to enter the cells; however, major differences were revealed in the inhibition of their endocytosis compared to Caco-2 cells. NF-kappa B p65 translocation was not detected in the cell nuclei after HPBCD or RAMEB pre-treatment and cyclodextrin treatment did not enhance the formation of autophagosomes. These cyclodextrin derivates were partially localized in lysosomes after internalization.https://www.mdpi.com/1420-3049/27/5/1589HPBCDRAMEBendocytosislysosomesautophagyNF-κB |
spellingShingle | Ágnes Rusznyák Mercédesz Palicskó Milo Malanga Éva Fenyvesi Lajos Szente Judit Váradi Ildikó Bácskay Miklós Vecsernyés Katalin Szászné Réti-Nagy Gábor Vasvári Ádám Haimhoffer Ferenc Fenyvesi Cellular Effects of Cyclodextrins: Studies on HeLa Cells Molecules HPBCD RAMEB endocytosis lysosomes autophagy NF-κB |
title | Cellular Effects of Cyclodextrins: Studies on HeLa Cells |
title_full | Cellular Effects of Cyclodextrins: Studies on HeLa Cells |
title_fullStr | Cellular Effects of Cyclodextrins: Studies on HeLa Cells |
title_full_unstemmed | Cellular Effects of Cyclodextrins: Studies on HeLa Cells |
title_short | Cellular Effects of Cyclodextrins: Studies on HeLa Cells |
title_sort | cellular effects of cyclodextrins studies on hela cells |
topic | HPBCD RAMEB endocytosis lysosomes autophagy NF-κB |
url | https://www.mdpi.com/1420-3049/27/5/1589 |
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