Gender Differences of NLRP1 Inflammasome in Mouse Model of Alzheimer's Disease
Background: There is a significant gender difference in the incidence and symptoms of Alzheimer's disease (AD), but its mechanisms are not completely understood. Recent studies showed that NLRP1 inflammasome was overexpressed in females under some pathological conditions such as nodular melanom...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-11-01
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| Series: | Frontiers in Aging Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnagi.2020.512097/full |
| _version_ | 1819007585716535296 |
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| author | Jinghang Zhang Jinghang Zhang Lihong Pei Dan Zang Yun Xue Xiaohui Wang Yiyang Chen Jinsong Li Jian Yu Qingzu Gao Wenyu Di Chaochu Cui Wei Su Xianwei Wang |
| author_facet | Jinghang Zhang Jinghang Zhang Lihong Pei Dan Zang Yun Xue Xiaohui Wang Yiyang Chen Jinsong Li Jian Yu Qingzu Gao Wenyu Di Chaochu Cui Wei Su Xianwei Wang |
| author_sort | Jinghang Zhang |
| collection | DOAJ |
| description | Background: There is a significant gender difference in the incidence and symptoms of Alzheimer's disease (AD), but its mechanisms are not completely understood. Recent studies showed that NLRP1 inflammasome was overexpressed in females under some pathological conditions such as nodular melanoma. Whether NLRP1 signals have a gender difference in AD has not been elucidated. This study was designed to investigate gender difference on the expressions of NLRP1 signals including NLRP1, Capase-1 and IL-1β in the brains of APP/PS1+/− mice.Methods: Female and male APP/PS1+/− mice (30-weeks-old) were used in this study. Amyloid-β (Aβ) plaques were stained with Congo red dye and cell apoptosis was detected by TUNEL staining. Expressions of NLRP1, Capase-1 and IL-1β were measured by immunofluorescent staining and Western blotting assay.Results: The numbers of Aβ plaques in cortex and hippocampus and neuronal apoptosis in cortex were 4 and 2-folds in females than males, respectively (P < 0.001). The average size of Aβ plaques in both cortex (females: 3527.11 ± 539.88 μm2 vs. males: 1920.44 ± 638.49 μm2) and hippocampus (females: 1931 ± 308.61 μm2 vs. males: 1038.55 ± 220.40 μm2) were also larger in females than males (P < 0.01). More interestingly, expressions of NLRP1, Caspase-1, and IL-1β were markedly increased in the cortex of females as compared with males.Conclusions: These findings show that NLRP1 signals are higher in brains of female APP/PS1+/− mice than males, which may be related to the gender differences of AD. |
| first_indexed | 2024-12-21T00:26:55Z |
| format | Article |
| id | doaj.art-6afd2eb95c6840a8a8ffd4514d365a10 |
| institution | Directory Open Access Journal |
| issn | 1663-4365 |
| language | English |
| last_indexed | 2024-12-21T00:26:55Z |
| publishDate | 2020-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Aging Neuroscience |
| spelling | doaj.art-6afd2eb95c6840a8a8ffd4514d365a102022-12-21T19:21:58ZengFrontiers Media S.A.Frontiers in Aging Neuroscience1663-43652020-11-011210.3389/fnagi.2020.512097512097Gender Differences of NLRP1 Inflammasome in Mouse Model of Alzheimer's DiseaseJinghang Zhang0Jinghang Zhang1Lihong Pei2Dan Zang3Yun Xue4Xiaohui Wang5Yiyang Chen6Jinsong Li7Jian Yu8Qingzu Gao9Wenyu Di10Chaochu Cui11Wei Su12Xianwei Wang13Department of Pathology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, ChinaKey Laboratory of Lymphohematopoietic Tumor in Xinxiang, Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, ChinaHenan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, ChinaDepartment of Pathology, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, ChinaHenan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, ChinaBackground: There is a significant gender difference in the incidence and symptoms of Alzheimer's disease (AD), but its mechanisms are not completely understood. Recent studies showed that NLRP1 inflammasome was overexpressed in females under some pathological conditions such as nodular melanoma. Whether NLRP1 signals have a gender difference in AD has not been elucidated. This study was designed to investigate gender difference on the expressions of NLRP1 signals including NLRP1, Capase-1 and IL-1β in the brains of APP/PS1+/− mice.Methods: Female and male APP/PS1+/− mice (30-weeks-old) were used in this study. Amyloid-β (Aβ) plaques were stained with Congo red dye and cell apoptosis was detected by TUNEL staining. Expressions of NLRP1, Capase-1 and IL-1β were measured by immunofluorescent staining and Western blotting assay.Results: The numbers of Aβ plaques in cortex and hippocampus and neuronal apoptosis in cortex were 4 and 2-folds in females than males, respectively (P < 0.001). The average size of Aβ plaques in both cortex (females: 3527.11 ± 539.88 μm2 vs. males: 1920.44 ± 638.49 μm2) and hippocampus (females: 1931 ± 308.61 μm2 vs. males: 1038.55 ± 220.40 μm2) were also larger in females than males (P < 0.01). More interestingly, expressions of NLRP1, Caspase-1, and IL-1β were markedly increased in the cortex of females as compared with males.Conclusions: These findings show that NLRP1 signals are higher in brains of female APP/PS1+/− mice than males, which may be related to the gender differences of AD.https://www.frontiersin.org/articles/10.3389/fnagi.2020.512097/fullAlzheimer's diseaseNLRP1 inflammasomegender differencesneuronal apoptosisAPP/PS1+/− mice |
| spellingShingle | Jinghang Zhang Jinghang Zhang Lihong Pei Dan Zang Yun Xue Xiaohui Wang Yiyang Chen Jinsong Li Jian Yu Qingzu Gao Wenyu Di Chaochu Cui Wei Su Xianwei Wang Gender Differences of NLRP1 Inflammasome in Mouse Model of Alzheimer's Disease Frontiers in Aging Neuroscience Alzheimer's disease NLRP1 inflammasome gender differences neuronal apoptosis APP/PS1+/− mice |
| title | Gender Differences of NLRP1 Inflammasome in Mouse Model of Alzheimer's Disease |
| title_full | Gender Differences of NLRP1 Inflammasome in Mouse Model of Alzheimer's Disease |
| title_fullStr | Gender Differences of NLRP1 Inflammasome in Mouse Model of Alzheimer's Disease |
| title_full_unstemmed | Gender Differences of NLRP1 Inflammasome in Mouse Model of Alzheimer's Disease |
| title_short | Gender Differences of NLRP1 Inflammasome in Mouse Model of Alzheimer's Disease |
| title_sort | gender differences of nlrp1 inflammasome in mouse model of alzheimer s disease |
| topic | Alzheimer's disease NLRP1 inflammasome gender differences neuronal apoptosis APP/PS1+/− mice |
| url | https://www.frontiersin.org/articles/10.3389/fnagi.2020.512097/full |
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