Butyrate Conversion by Sulfate-Reducing and Methanogenic Communities from Anoxic Sediments of Aarhus Bay, Denmark
The conventional perception that the zone of sulfate reduction and methanogenesis are separated in high- and low-sulfate-containing marine sediments has recently been changed by studies demonstrating their co-occurrence in sediments. The presence of methanogens was linked to the presence of substrat...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/8/4/606 |
| _version_ | 1827718152259108864 |
|---|---|
| author | Derya Ozuolmez Elisha K. Moore Ellen C. Hopmans Jaap S. Sinninghe Damsté Alfons J. M. Stams Caroline M. Plugge |
| author_facet | Derya Ozuolmez Elisha K. Moore Ellen C. Hopmans Jaap S. Sinninghe Damsté Alfons J. M. Stams Caroline M. Plugge |
| author_sort | Derya Ozuolmez |
| collection | DOAJ |
| description | The conventional perception that the zone of sulfate reduction and methanogenesis are separated in high- and low-sulfate-containing marine sediments has recently been changed by studies demonstrating their co-occurrence in sediments. The presence of methanogens was linked to the presence of substrates that are not used by sulfate reducers. In the current study, we hypothesized that both groups can co-exist, consuming common substrates (H<sub>2</sub> and/or acetate) in sediments. We enriched butyrate-degrading communities in sediment slurries originating from the sulfate, sulfate–methane transition, and methane zone of Aarhus Bay, Denmark. Sulfate was added at different concentrations (0, 3, 20 mM), and the slurries were incubated at 10 °C and 25 °C. During butyrate conversion, sulfate reduction and methanogenesis occurred simultaneously. The syntrophic butyrate degrader <i>Syntrophomonas</i> was enriched both in sulfate-amended and in sulfate-free slurries, indicating the occurrence of syntrophic conversions at both conditions. Archaeal community analysis revealed a dominance of <i>Methanomicrobiaceae</i>. The acetoclastic <i>Methanosaetaceae</i> reached high relative abundance in the absence of sulfate, while presence of acetoclastic <i>Methanosarcinaceae</i> was independent of the sulfate concentration, temperature, and the initial zone of the sediment. This study shows that there is no vertical separation of sulfate reducers, syntrophs, and methanogens in the sediment and that they all participate in the conversion of butyrate. |
| first_indexed | 2024-03-10T20:18:22Z |
| format | Article |
| id | doaj.art-6afdc78ba03948fdbe1f00b5224b51b4 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T20:18:22Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-6afdc78ba03948fdbe1f00b5224b51b42023-11-19T22:23:06ZengMDPI AGMicroorganisms2076-26072020-04-018460610.3390/microorganisms8040606Butyrate Conversion by Sulfate-Reducing and Methanogenic Communities from Anoxic Sediments of Aarhus Bay, DenmarkDerya Ozuolmez0Elisha K. Moore1Ellen C. Hopmans2Jaap S. Sinninghe Damsté3Alfons J. M. Stams4Caroline M. Plugge5Laboratory of Microbiology, Wageningen University & Research, 6708 WE Wageningen, The NetherlandsDepartment of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, and Utrecht University, P.O. Box 59, 1790 AB Den Burg, The NetherlandsDepartment of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, and Utrecht University, P.O. Box 59, 1790 AB Den Burg, The NetherlandsDepartment of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, and Utrecht University, P.O. Box 59, 1790 AB Den Burg, The NetherlandsLaboratory of Microbiology, Wageningen University & Research, 6708 WE Wageningen, The NetherlandsLaboratory of Microbiology, Wageningen University & Research, 6708 WE Wageningen, The NetherlandsThe conventional perception that the zone of sulfate reduction and methanogenesis are separated in high- and low-sulfate-containing marine sediments has recently been changed by studies demonstrating their co-occurrence in sediments. The presence of methanogens was linked to the presence of substrates that are not used by sulfate reducers. In the current study, we hypothesized that both groups can co-exist, consuming common substrates (H<sub>2</sub> and/or acetate) in sediments. We enriched butyrate-degrading communities in sediment slurries originating from the sulfate, sulfate–methane transition, and methane zone of Aarhus Bay, Denmark. Sulfate was added at different concentrations (0, 3, 20 mM), and the slurries were incubated at 10 °C and 25 °C. During butyrate conversion, sulfate reduction and methanogenesis occurred simultaneously. The syntrophic butyrate degrader <i>Syntrophomonas</i> was enriched both in sulfate-amended and in sulfate-free slurries, indicating the occurrence of syntrophic conversions at both conditions. Archaeal community analysis revealed a dominance of <i>Methanomicrobiaceae</i>. The acetoclastic <i>Methanosaetaceae</i> reached high relative abundance in the absence of sulfate, while presence of acetoclastic <i>Methanosarcinaceae</i> was independent of the sulfate concentration, temperature, and the initial zone of the sediment. This study shows that there is no vertical separation of sulfate reducers, syntrophs, and methanogens in the sediment and that they all participate in the conversion of butyrate.https://www.mdpi.com/2076-2607/8/4/606marine sedimentsulfate-reducing bacteriamethanogenic archaeasyntrophyintact polar lipidsAarhus Bay |
| spellingShingle | Derya Ozuolmez Elisha K. Moore Ellen C. Hopmans Jaap S. Sinninghe Damsté Alfons J. M. Stams Caroline M. Plugge Butyrate Conversion by Sulfate-Reducing and Methanogenic Communities from Anoxic Sediments of Aarhus Bay, Denmark Microorganisms marine sediment sulfate-reducing bacteria methanogenic archaea syntrophy intact polar lipids Aarhus Bay |
| title | Butyrate Conversion by Sulfate-Reducing and Methanogenic Communities from Anoxic Sediments of Aarhus Bay, Denmark |
| title_full | Butyrate Conversion by Sulfate-Reducing and Methanogenic Communities from Anoxic Sediments of Aarhus Bay, Denmark |
| title_fullStr | Butyrate Conversion by Sulfate-Reducing and Methanogenic Communities from Anoxic Sediments of Aarhus Bay, Denmark |
| title_full_unstemmed | Butyrate Conversion by Sulfate-Reducing and Methanogenic Communities from Anoxic Sediments of Aarhus Bay, Denmark |
| title_short | Butyrate Conversion by Sulfate-Reducing and Methanogenic Communities from Anoxic Sediments of Aarhus Bay, Denmark |
| title_sort | butyrate conversion by sulfate reducing and methanogenic communities from anoxic sediments of aarhus bay denmark |
| topic | marine sediment sulfate-reducing bacteria methanogenic archaea syntrophy intact polar lipids Aarhus Bay |
| url | https://www.mdpi.com/2076-2607/8/4/606 |
| work_keys_str_mv | AT deryaozuolmez butyrateconversionbysulfatereducingandmethanogeniccommunitiesfromanoxicsedimentsofaarhusbaydenmark AT elishakmoore butyrateconversionbysulfatereducingandmethanogeniccommunitiesfromanoxicsedimentsofaarhusbaydenmark AT ellenchopmans butyrateconversionbysulfatereducingandmethanogeniccommunitiesfromanoxicsedimentsofaarhusbaydenmark AT jaapssinninghedamste butyrateconversionbysulfatereducingandmethanogeniccommunitiesfromanoxicsedimentsofaarhusbaydenmark AT alfonsjmstams butyrateconversionbysulfatereducingandmethanogeniccommunitiesfromanoxicsedimentsofaarhusbaydenmark AT carolinemplugge butyrateconversionbysulfatereducingandmethanogeniccommunitiesfromanoxicsedimentsofaarhusbaydenmark |