Treatment of Human HeLa Cells with Black Raspberry Extracts Enhances the Removal of DNA Lesions by the Nucleotide Excision Repair Mechanism
As demonstrated by us earlier and by other researchers, a diet containing freeze-dried black raspberries (BRB) inhibits DNA damage and carcinogenesis in animal models. We tested the hypothesis that the inhibition of DNA damage by BRB is due, in part, to the enhancement of DNA repair capacity evaluat...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/11/2110 |
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| author | Ana H. Sales Marina Kolbanovskiy Nicholas E. Geacintov Kun-Ming Chen Yuan-Wan Sun Karam El-Bayoumy |
| author_facet | Ana H. Sales Marina Kolbanovskiy Nicholas E. Geacintov Kun-Ming Chen Yuan-Wan Sun Karam El-Bayoumy |
| author_sort | Ana H. Sales |
| collection | DOAJ |
| description | As demonstrated by us earlier and by other researchers, a diet containing freeze-dried black raspberries (BRB) inhibits DNA damage and carcinogenesis in animal models. We tested the hypothesis that the inhibition of DNA damage by BRB is due, in part, to the enhancement of DNA repair capacity evaluated in the human HeLa cell extract system, an established in vitro system for the assessment of cellular DNA repair activity. The pre-treatment of intact HeLa cells with BRB extracts (BRBE) enhances the nucleotide excision repair (NER) of a bulky deoxyguanosine adduct derived from the polycyclic aromatic carcinogen benzo[<i>a</i>]pyrene (BP-dG) by ~24%. The NER activity of an oxidatively-derived non-bulky DNA lesion, guanidinohydantoin (Gh), is also enhanced by ~24%, while its base excision repair activity is enhanced by only ~6%. Western Blot experiments indicate that the expression of selected, NER factors is also increased by BRBE treatment by ~73% (XPA), ~55% (XPB), while its effects on XPD was modest (<14%). These results demonstrate that BRBE significantly enhances the NER yields of a bulky and a non-bulky DNA lesion, and that this effect is correlated with an enhancement of expression of the critically important NER factor XPA and the helicase XPB, but not the helicase XPD. |
| first_indexed | 2024-03-09T19:19:05Z |
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| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T19:19:05Z |
| publishDate | 2022-10-01 |
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| series | Antioxidants |
| spelling | doaj.art-6affea84318e48c6a9ff316d2ca9194b2023-11-24T03:29:23ZengMDPI AGAntioxidants2076-39212022-10-011111211010.3390/antiox11112110Treatment of Human HeLa Cells with Black Raspberry Extracts Enhances the Removal of DNA Lesions by the Nucleotide Excision Repair MechanismAna H. Sales0Marina Kolbanovskiy1Nicholas E. Geacintov2Kun-Ming Chen3Yuan-Wan Sun4Karam El-Bayoumy5Department of Chemistry, New York University, New York, NY 10003, USADepartment of Chemistry, New York University, New York, NY 10003, USADepartment of Chemistry, New York University, New York, NY 10003, USADepartment of Biochemistry and Molecular Biology, College of Medicine, Pennsylvania State University, Hershey, PA 17033, USADepartment of Biochemistry and Molecular Biology, College of Medicine, Pennsylvania State University, Hershey, PA 17033, USADepartment of Biochemistry and Molecular Biology, College of Medicine, Pennsylvania State University, Hershey, PA 17033, USAAs demonstrated by us earlier and by other researchers, a diet containing freeze-dried black raspberries (BRB) inhibits DNA damage and carcinogenesis in animal models. We tested the hypothesis that the inhibition of DNA damage by BRB is due, in part, to the enhancement of DNA repair capacity evaluated in the human HeLa cell extract system, an established in vitro system for the assessment of cellular DNA repair activity. The pre-treatment of intact HeLa cells with BRB extracts (BRBE) enhances the nucleotide excision repair (NER) of a bulky deoxyguanosine adduct derived from the polycyclic aromatic carcinogen benzo[<i>a</i>]pyrene (BP-dG) by ~24%. The NER activity of an oxidatively-derived non-bulky DNA lesion, guanidinohydantoin (Gh), is also enhanced by ~24%, while its base excision repair activity is enhanced by only ~6%. Western Blot experiments indicate that the expression of selected, NER factors is also increased by BRBE treatment by ~73% (XPA), ~55% (XPB), while its effects on XPD was modest (<14%). These results demonstrate that BRBE significantly enhances the NER yields of a bulky and a non-bulky DNA lesion, and that this effect is correlated with an enhancement of expression of the critically important NER factor XPA and the helicase XPB, but not the helicase XPD.https://www.mdpi.com/2076-3921/11/11/2110black raspberry extractsoxidative DNA damage and repairbulky benzo[<i>a</i>]pyrene DNA adductsexpression of nucleotide excision repair proteinswestern blots |
| spellingShingle | Ana H. Sales Marina Kolbanovskiy Nicholas E. Geacintov Kun-Ming Chen Yuan-Wan Sun Karam El-Bayoumy Treatment of Human HeLa Cells with Black Raspberry Extracts Enhances the Removal of DNA Lesions by the Nucleotide Excision Repair Mechanism Antioxidants black raspberry extracts oxidative DNA damage and repair bulky benzo[<i>a</i>]pyrene DNA adducts expression of nucleotide excision repair proteins western blots |
| title | Treatment of Human HeLa Cells with Black Raspberry Extracts Enhances the Removal of DNA Lesions by the Nucleotide Excision Repair Mechanism |
| title_full | Treatment of Human HeLa Cells with Black Raspberry Extracts Enhances the Removal of DNA Lesions by the Nucleotide Excision Repair Mechanism |
| title_fullStr | Treatment of Human HeLa Cells with Black Raspberry Extracts Enhances the Removal of DNA Lesions by the Nucleotide Excision Repair Mechanism |
| title_full_unstemmed | Treatment of Human HeLa Cells with Black Raspberry Extracts Enhances the Removal of DNA Lesions by the Nucleotide Excision Repair Mechanism |
| title_short | Treatment of Human HeLa Cells with Black Raspberry Extracts Enhances the Removal of DNA Lesions by the Nucleotide Excision Repair Mechanism |
| title_sort | treatment of human hela cells with black raspberry extracts enhances the removal of dna lesions by the nucleotide excision repair mechanism |
| topic | black raspberry extracts oxidative DNA damage and repair bulky benzo[<i>a</i>]pyrene DNA adducts expression of nucleotide excision repair proteins western blots |
| url | https://www.mdpi.com/2076-3921/11/11/2110 |
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