Influence of Oxidative Stress on Time-Resolved Oxygen Detection by [Ru(Phen)<sub>3</sub>]<sup>2+</sup> In Vivo and In Vitro
Detection of tissue and cell oxygenation is of high importance in fundamental biological and in many medical applications, particularly for monitoring dysfunction in the early stages of cancer. Measurements of the luminescence lifetimes of molecular probes offer a very promising and non-invasive app...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/2/485 |
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| author | Veronika Huntosova Denis Horvath Robert Seliga Georges Wagnieres |
| author_facet | Veronika Huntosova Denis Horvath Robert Seliga Georges Wagnieres |
| author_sort | Veronika Huntosova |
| collection | DOAJ |
| description | Detection of tissue and cell oxygenation is of high importance in fundamental biological and in many medical applications, particularly for monitoring dysfunction in the early stages of cancer. Measurements of the luminescence lifetimes of molecular probes offer a very promising and non-invasive approach to estimate tissue and cell oxygenation in vivo and in vitro. We optimized the evaluation of oxygen detection in vivo by [Ru(Phen)<sub>3</sub>]<sup>2+</sup> in the chicken embryo chorioallantoic membrane model. Its luminescence lifetimes measured in the CAM were analyzed through hierarchical clustering. The detection of the tissue oxygenation at the oxidative stress conditions is still challenging. We applied simultaneous time-resolved recording of the mitochondrial probe MitoTracker<sup>TM</sup> OrangeCMTMRos fluorescence and [Ru(Phen)<sub>3</sub>]<sup>2+</sup> phosphorescence imaging in the intact cell without affecting the sensitivities of these molecular probes. [Ru(Phen)<sub>3</sub>]<sup>2+</sup> was demonstrated to be suitable for in vitro detection of oxygen under various stress factors that mimic oxidative stress: other molecular sensors, H<sub>2</sub>O<sub>2</sub>, and curcumin-mediated photodynamic therapy in glioma cancer cells. Low phototoxicities of the molecular probes were finally observed. Our study offers a high potential for the application and generalization of tissue oxygenation as an innovative approach based on the similarities between interdependent biological influences. It is particularly suitable for therapeutic approaches targeting metabolic alterations as well as oxygen, glucose, or lipid deprivation. |
| first_indexed | 2024-03-09T04:26:49Z |
| format | Article |
| id | doaj.art-6b01f54b81e04ede907bd81ed046677f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T04:26:49Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6b01f54b81e04ede907bd81ed046677f2023-12-03T13:39:49ZengMDPI AGMolecules1420-30492021-01-0126248510.3390/molecules26020485Influence of Oxidative Stress on Time-Resolved Oxygen Detection by [Ru(Phen)<sub>3</sub>]<sup>2+</sup> In Vivo and In VitroVeronika Huntosova0Denis Horvath1Robert Seliga2Georges Wagnieres3Center for Interdisciplinary Biosciences, Technology and Innovation Park, P.J. Safarik University in Kosice, Jesenna 5, 041 54 Kosice, SlovakiaCenter for Interdisciplinary Biosciences, Technology and Innovation Park, P.J. Safarik University in Kosice, Jesenna 5, 041 54 Kosice, SlovakiaCenter for Interdisciplinary Biosciences, Technology and Innovation Park, P.J. Safarik University in Kosice, Jesenna 5, 041 54 Kosice, SlovakiaLaboratory for Functional and Metabolic Imaging, Institute of Physics, Swiss Federal Institute of Technology in Lausanne (EPFL), Station 6, Batiment de Chimie, 1015 Lausanne, SwitzerlandDetection of tissue and cell oxygenation is of high importance in fundamental biological and in many medical applications, particularly for monitoring dysfunction in the early stages of cancer. Measurements of the luminescence lifetimes of molecular probes offer a very promising and non-invasive approach to estimate tissue and cell oxygenation in vivo and in vitro. We optimized the evaluation of oxygen detection in vivo by [Ru(Phen)<sub>3</sub>]<sup>2+</sup> in the chicken embryo chorioallantoic membrane model. Its luminescence lifetimes measured in the CAM were analyzed through hierarchical clustering. The detection of the tissue oxygenation at the oxidative stress conditions is still challenging. We applied simultaneous time-resolved recording of the mitochondrial probe MitoTracker<sup>TM</sup> OrangeCMTMRos fluorescence and [Ru(Phen)<sub>3</sub>]<sup>2+</sup> phosphorescence imaging in the intact cell without affecting the sensitivities of these molecular probes. [Ru(Phen)<sub>3</sub>]<sup>2+</sup> was demonstrated to be suitable for in vitro detection of oxygen under various stress factors that mimic oxidative stress: other molecular sensors, H<sub>2</sub>O<sub>2</sub>, and curcumin-mediated photodynamic therapy in glioma cancer cells. Low phototoxicities of the molecular probes were finally observed. Our study offers a high potential for the application and generalization of tissue oxygenation as an innovative approach based on the similarities between interdependent biological influences. It is particularly suitable for therapeutic approaches targeting metabolic alterations as well as oxygen, glucose, or lipid deprivation.https://www.mdpi.com/1420-3049/26/2/485oxygen detectiondendrogram analysishierarchical clusteringcancer cellsluminescence lifetimehydrogen peroxide |
| spellingShingle | Veronika Huntosova Denis Horvath Robert Seliga Georges Wagnieres Influence of Oxidative Stress on Time-Resolved Oxygen Detection by [Ru(Phen)<sub>3</sub>]<sup>2+</sup> In Vivo and In Vitro Molecules oxygen detection dendrogram analysis hierarchical clustering cancer cells luminescence lifetime hydrogen peroxide |
| title | Influence of Oxidative Stress on Time-Resolved Oxygen Detection by [Ru(Phen)<sub>3</sub>]<sup>2+</sup> In Vivo and In Vitro |
| title_full | Influence of Oxidative Stress on Time-Resolved Oxygen Detection by [Ru(Phen)<sub>3</sub>]<sup>2+</sup> In Vivo and In Vitro |
| title_fullStr | Influence of Oxidative Stress on Time-Resolved Oxygen Detection by [Ru(Phen)<sub>3</sub>]<sup>2+</sup> In Vivo and In Vitro |
| title_full_unstemmed | Influence of Oxidative Stress on Time-Resolved Oxygen Detection by [Ru(Phen)<sub>3</sub>]<sup>2+</sup> In Vivo and In Vitro |
| title_short | Influence of Oxidative Stress on Time-Resolved Oxygen Detection by [Ru(Phen)<sub>3</sub>]<sup>2+</sup> In Vivo and In Vitro |
| title_sort | influence of oxidative stress on time resolved oxygen detection by ru phen sub 3 sub sup 2 sup in vivo and in vitro |
| topic | oxygen detection dendrogram analysis hierarchical clustering cancer cells luminescence lifetime hydrogen peroxide |
| url | https://www.mdpi.com/1420-3049/26/2/485 |
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