Polymorphisms of the PRLR Gene and Their Association with Milk Production Traits in Egyptian Buffaloes
Prolactin (PRL) and its receptor (PRLR) were considered as potential genetic markers for milk production and quality traits in cattle. However, little information is available regarding <i>PRLR</i> genetic diversity and association studies with milk traits in Egyptian water buffaloes. Th...
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MDPI AG
2021-04-01
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| author | Mohammed A. El-Magd Aziza Fathy Khaled A. Kahilo Ayman A. Saleh Ahmed I. El Sheikh Salah AL-Shami Shymaa M. El-Komy |
| author_facet | Mohammed A. El-Magd Aziza Fathy Khaled A. Kahilo Ayman A. Saleh Ahmed I. El Sheikh Salah AL-Shami Shymaa M. El-Komy |
| author_sort | Mohammed A. El-Magd |
| collection | DOAJ |
| description | Prolactin (PRL) and its receptor (PRLR) were considered as potential genetic markers for milk production and quality traits in cattle. However, little information is available regarding <i>PRLR</i> genetic diversity and association studies with milk traits in Egyptian water buffaloes. Therefore, the present study was conducted to search for mutations in <i>PRLR</i> and determine their associations with milk performance in these animals. Exon3 (E3) and E10 of <i>PRLR</i> were screened for polymorphisms using single strand conformation polymorphism (SSCP) and sequencing in 400 buffaloes. The associations between haplotypes and milk production (fat%, protein%, lactose%, and solid%) traits as well as mRNA and protein levels of PRL and PRLR were studied. Two single nucleotide polymorphisms (SNPs) in E10 were detected: g.11685G>A (p.Ala494Thr) and g.11773T>C (p.Val523Aal). The G and T alleles were wild (ancestral) alleles, while the A and C alleles were mutant alleles. These SNPs resulted in four haplotypes; AC, AT, GC, and GT. Buffaloes with wild GT haplotypes showed significantly higher milk yield, fat% and protein%, mRNA and protein levels of PRL and PRLR in milk somatic cells than other animals. Animals carrying mutant AC haplotype had inferior milk traits and lowest levels of associated mRNAs and proteins. With these results, we could conclude that the selection of buffaloes with wild GT haplotypes for g.11685G>A and g.11773T>C SNPs of the <i>PRLR</i> gene might improve the milk production traits of Egyptian water buffaloes. |
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| last_indexed | 2024-03-10T11:58:57Z |
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| spelling | doaj.art-6b040c906af84adc8917295f2cc37e6b2023-11-21T17:04:42ZengMDPI AGAnimals2076-26152021-04-01115123710.3390/ani11051237Polymorphisms of the PRLR Gene and Their Association with Milk Production Traits in Egyptian BuffaloesMohammed A. El-Magd0Aziza Fathy1Khaled A. Kahilo2Ayman A. Saleh3Ahmed I. El Sheikh4Salah AL-Shami5Shymaa M. El-Komy6Department of Anatomy & Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, EgyptDepartment of Biochemistry, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, EgyptDepartment of Biochemistry, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, EgyptDepartment of Animal Wealth Development, Veterinary Genetics & Genetic Engineering, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, EgyptDepartment of Public Health, Faculty of Veterinary Medicine, King Faisal University, Alahssa 31982, Saudi ArabiaDepartment of Public Health, Faculty of Veterinary Medicine, King Faisal University, Alahssa 31982, Saudi ArabiaDepartment of Animal Production, Faculty of Agriculture, Tanta University, Tanta 31527, EgyptProlactin (PRL) and its receptor (PRLR) were considered as potential genetic markers for milk production and quality traits in cattle. However, little information is available regarding <i>PRLR</i> genetic diversity and association studies with milk traits in Egyptian water buffaloes. Therefore, the present study was conducted to search for mutations in <i>PRLR</i> and determine their associations with milk performance in these animals. Exon3 (E3) and E10 of <i>PRLR</i> were screened for polymorphisms using single strand conformation polymorphism (SSCP) and sequencing in 400 buffaloes. The associations between haplotypes and milk production (fat%, protein%, lactose%, and solid%) traits as well as mRNA and protein levels of PRL and PRLR were studied. Two single nucleotide polymorphisms (SNPs) in E10 were detected: g.11685G>A (p.Ala494Thr) and g.11773T>C (p.Val523Aal). The G and T alleles were wild (ancestral) alleles, while the A and C alleles were mutant alleles. These SNPs resulted in four haplotypes; AC, AT, GC, and GT. Buffaloes with wild GT haplotypes showed significantly higher milk yield, fat% and protein%, mRNA and protein levels of PRL and PRLR in milk somatic cells than other animals. Animals carrying mutant AC haplotype had inferior milk traits and lowest levels of associated mRNAs and proteins. With these results, we could conclude that the selection of buffaloes with wild GT haplotypes for g.11685G>A and g.11773T>C SNPs of the <i>PRLR</i> gene might improve the milk production traits of Egyptian water buffaloes.https://www.mdpi.com/2076-2615/11/5/1237prolactin receptormutationsEgyptian buffalomilk performance |
| spellingShingle | Mohammed A. El-Magd Aziza Fathy Khaled A. Kahilo Ayman A. Saleh Ahmed I. El Sheikh Salah AL-Shami Shymaa M. El-Komy Polymorphisms of the PRLR Gene and Their Association with Milk Production Traits in Egyptian Buffaloes Animals prolactin receptor mutations Egyptian buffalo milk performance |
| title | Polymorphisms of the PRLR Gene and Their Association with Milk Production Traits in Egyptian Buffaloes |
| title_full | Polymorphisms of the PRLR Gene and Their Association with Milk Production Traits in Egyptian Buffaloes |
| title_fullStr | Polymorphisms of the PRLR Gene and Their Association with Milk Production Traits in Egyptian Buffaloes |
| title_full_unstemmed | Polymorphisms of the PRLR Gene and Their Association with Milk Production Traits in Egyptian Buffaloes |
| title_short | Polymorphisms of the PRLR Gene and Their Association with Milk Production Traits in Egyptian Buffaloes |
| title_sort | polymorphisms of the prlr gene and their association with milk production traits in egyptian buffaloes |
| topic | prolactin receptor mutations Egyptian buffalo milk performance |
| url | https://www.mdpi.com/2076-2615/11/5/1237 |
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