Case Report: Oral and fecal microbiota in a super-donor: the healthy microbiota paradigm for fecal transplantation
Despite the numerous fecal microbiota transplantation trials that have been carried out, knowledge about the actual composition of a “healthy microbiota” remains scarce. The aim of this research was to describe the differences in the composition of oral and fecal microbiotas in a super-donor. The mi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Microbiomes |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/frmbi.2023.1219960/full |
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| author | Nayeli Ortiz-Olvera Edith A. Fernández-Figueroa Jesús Argueta-Donohué Haydee Miranda-Ortíz Erika Ruiz-García Erika Ruiz-García |
| author_facet | Nayeli Ortiz-Olvera Edith A. Fernández-Figueroa Jesús Argueta-Donohué Haydee Miranda-Ortíz Erika Ruiz-García Erika Ruiz-García |
| author_sort | Nayeli Ortiz-Olvera |
| collection | DOAJ |
| description | Despite the numerous fecal microbiota transplantation trials that have been carried out, knowledge about the actual composition of a “healthy microbiota” remains scarce. The aim of this research was to describe the differences in the composition of oral and fecal microbiotas in a super-donor. The microbiota analysis was done using next-generation sequencing of the V3 and V4 regions of the bacterial 16S rRNA gene. The biodiversity found in the mouth was very rich, with 56 species identified, and there was a predominance of the genera Veillonella, Haemophilus, and Streptococcus. It is worth mentioning the presence (2.33%) of Fusobacterium nucleatum in the mouth. In feces, the genera Bacteroides and Faecalibacterium predominated, with the species F. prausnitzii being the most abundant. This analysis shows that the diversity of the microbiota of a super-donor plays a fundamental role in the effectiveness of its product in fecal matter transplantation. This suggests that transplanted gut microorganisms have the ability to maintain or recover health in a dynamic process between the microbiota and the host. Our findings encourage further research which will result in the development of bacterial therapies in infectious and inflammatory diseases. |
| first_indexed | 2024-03-12T01:20:29Z |
| format | Article |
| id | doaj.art-6b055e17a81a4722910ed134b42aca69 |
| institution | Directory Open Access Journal |
| issn | 2813-4338 |
| language | English |
| last_indexed | 2025-03-21T00:30:17Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiomes |
| spelling | doaj.art-6b055e17a81a4722910ed134b42aca692024-08-03T07:05:10ZengFrontiers Media S.A.Frontiers in Microbiomes2813-43382023-09-01210.3389/frmbi.2023.12199601219960Case Report: Oral and fecal microbiota in a super-donor: the healthy microbiota paradigm for fecal transplantationNayeli Ortiz-Olvera0Edith A. Fernández-Figueroa1Jesús Argueta-Donohué2Haydee Miranda-Ortíz3Erika Ruiz-García4Erika Ruiz-García5Departamento de Gastroenterología, UMAE, Hospital de Especialidades, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de Mexico, MexicoNúcleo B de Innovación en Medicina de Precisión. Instituto Nacional de Medicina Genómica, Ciudad de México, MexicoLaboratorio de Neurofarmacología, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Ciudad de México, MexicoUnidad de Secuenciación, Instituto Nacional de Medicina Genómica, Ciudad de México, MexicoDepartamento de Tumores de Tubo Digestivo, Instituto Nacional de Cancerología, Ciudad de México, MexicoLaboratorio de Medicina Traslacional, Instituto Nacional de Cancerología, Ciudad de México, MexicoDespite the numerous fecal microbiota transplantation trials that have been carried out, knowledge about the actual composition of a “healthy microbiota” remains scarce. The aim of this research was to describe the differences in the composition of oral and fecal microbiotas in a super-donor. The microbiota analysis was done using next-generation sequencing of the V3 and V4 regions of the bacterial 16S rRNA gene. The biodiversity found in the mouth was very rich, with 56 species identified, and there was a predominance of the genera Veillonella, Haemophilus, and Streptococcus. It is worth mentioning the presence (2.33%) of Fusobacterium nucleatum in the mouth. In feces, the genera Bacteroides and Faecalibacterium predominated, with the species F. prausnitzii being the most abundant. This analysis shows that the diversity of the microbiota of a super-donor plays a fundamental role in the effectiveness of its product in fecal matter transplantation. This suggests that transplanted gut microorganisms have the ability to maintain or recover health in a dynamic process between the microbiota and the host. Our findings encourage further research which will result in the development of bacterial therapies in infectious and inflammatory diseases.https://www.frontiersin.org/articles/10.3389/frmbi.2023.1219960/fulloral microbiotafecal microbiotaF. prausnitziiF. nucleatumC. difficile |
| spellingShingle | Nayeli Ortiz-Olvera Edith A. Fernández-Figueroa Jesús Argueta-Donohué Haydee Miranda-Ortíz Erika Ruiz-García Erika Ruiz-García Case Report: Oral and fecal microbiota in a super-donor: the healthy microbiota paradigm for fecal transplantation Frontiers in Microbiomes oral microbiota fecal microbiota F. prausnitzii F. nucleatum C. difficile |
| title | Case Report: Oral and fecal microbiota in a super-donor: the healthy microbiota paradigm for fecal transplantation |
| title_full | Case Report: Oral and fecal microbiota in a super-donor: the healthy microbiota paradigm for fecal transplantation |
| title_fullStr | Case Report: Oral and fecal microbiota in a super-donor: the healthy microbiota paradigm for fecal transplantation |
| title_full_unstemmed | Case Report: Oral and fecal microbiota in a super-donor: the healthy microbiota paradigm for fecal transplantation |
| title_short | Case Report: Oral and fecal microbiota in a super-donor: the healthy microbiota paradigm for fecal transplantation |
| title_sort | case report oral and fecal microbiota in a super donor the healthy microbiota paradigm for fecal transplantation |
| topic | oral microbiota fecal microbiota F. prausnitzii F. nucleatum C. difficile |
| url | https://www.frontiersin.org/articles/10.3389/frmbi.2023.1219960/full |
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