Bioflocculants’ production in a biomass-degrading bacterium using untreated corn stover as carbon source and use of bioflocculants for microalgae harvest
Abstract Background Bioflocculation has been developed as a cost-effective and environment-friendly method to harvest multiple microalgae. However, the high production cost of bioflocculants makes it difficult to scale up. In the current study, low-cost bioflocculants were produced from untreated co...
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| Format: | Article |
| Language: | English |
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BMC
2017-12-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-017-0987-6 |
| _version_ | 1811336945521917952 |
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| author | Haipeng Guo Chuntao Hong Bingsong Zheng Fan Lu Dean Jiang Wensheng Qin |
| author_facet | Haipeng Guo Chuntao Hong Bingsong Zheng Fan Lu Dean Jiang Wensheng Qin |
| author_sort | Haipeng Guo |
| collection | DOAJ |
| description | Abstract Background Bioflocculation has been developed as a cost-effective and environment-friendly method to harvest multiple microalgae. However, the high production cost of bioflocculants makes it difficult to scale up. In the current study, low-cost bioflocculants were produced from untreated corn stover by a biomass-degrading bacterium Pseudomonas sp. GO2. Results Pseudomonas sp. GO2 showed excellent production ability of bioflocculants through directly hydrolyzing various biomasses. The untreated corn stover was selected as carbon source for bioflocculants’ production due to its highest flocculating efficiency compared to that when using other biomasses as carbon source. The effects of fermentation parameters on bioflocculants’ production were optimized via response surface methodology. According to the optimal model, an ideal flocculating efficiency of 99.8% was obtained with the fermentation time of 130.46 h, initial pH of 7.46, and biomass content of 0.64%. The relative importance of carboxymethyl cellulase and xylanase accounted for 51.8% in the process of bioflocculants’ production by boosted regression tree analysis, further indicating that the bioflocculants were mainly from the hydrolysates of biomass. Biochemical analysis showed that it contained 59.0% polysaccharides with uronic acid (34.2%), 32.1% protein, and 6.1% nucleic acid in the bioflocculants, which had an average molecular weight as 1.33 × 106 Da. In addition, the bioflocculants showed the highest flocculating efficiency at a concentration of 12.5 mg L−1 and were stable over broad ranges of pH and temperature. The highest flocculating efficiencies obtained for Chlorella zofingiensis and Neochloris oleoabundans were 77.9 and 88.9%, respectively. Conclusions The results indicated that Pseudomonas sp. GO2 can directly utilize various untreated lignocellulolytic biomasses to produce low-cost bioflocculants, which showed the high efficiency to harvest two green microalgae in a low GO2 fermentation broth/algal culture ratio. |
| first_indexed | 2024-04-13T17:46:55Z |
| format | Article |
| id | doaj.art-6b07c99bef2a47cabf345dda014b9630 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-13T17:46:55Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-6b07c99bef2a47cabf345dda014b96302022-12-22T02:36:55ZengBMCBiotechnology for Biofuels1754-68342017-12-0110111210.1186/s13068-017-0987-6Bioflocculants’ production in a biomass-degrading bacterium using untreated corn stover as carbon source and use of bioflocculants for microalgae harvestHaipeng Guo0Chuntao Hong1Bingsong Zheng2Fan Lu3Dean Jiang4Wensheng Qin5Department of Biology, Lakehead UniversityAcademy of Agricultural Sciences of Ningbo CityState Key Laboratory of Subtropical Silviculture, Zhejiang A & F UniversitySchool of Biological Engineering, Hubei University of TechnologyState Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang UniversityDepartment of Biology, Lakehead UniversityAbstract Background Bioflocculation has been developed as a cost-effective and environment-friendly method to harvest multiple microalgae. However, the high production cost of bioflocculants makes it difficult to scale up. In the current study, low-cost bioflocculants were produced from untreated corn stover by a biomass-degrading bacterium Pseudomonas sp. GO2. Results Pseudomonas sp. GO2 showed excellent production ability of bioflocculants through directly hydrolyzing various biomasses. The untreated corn stover was selected as carbon source for bioflocculants’ production due to its highest flocculating efficiency compared to that when using other biomasses as carbon source. The effects of fermentation parameters on bioflocculants’ production were optimized via response surface methodology. According to the optimal model, an ideal flocculating efficiency of 99.8% was obtained with the fermentation time of 130.46 h, initial pH of 7.46, and biomass content of 0.64%. The relative importance of carboxymethyl cellulase and xylanase accounted for 51.8% in the process of bioflocculants’ production by boosted regression tree analysis, further indicating that the bioflocculants were mainly from the hydrolysates of biomass. Biochemical analysis showed that it contained 59.0% polysaccharides with uronic acid (34.2%), 32.1% protein, and 6.1% nucleic acid in the bioflocculants, which had an average molecular weight as 1.33 × 106 Da. In addition, the bioflocculants showed the highest flocculating efficiency at a concentration of 12.5 mg L−1 and were stable over broad ranges of pH and temperature. The highest flocculating efficiencies obtained for Chlorella zofingiensis and Neochloris oleoabundans were 77.9 and 88.9%, respectively. Conclusions The results indicated that Pseudomonas sp. GO2 can directly utilize various untreated lignocellulolytic biomasses to produce low-cost bioflocculants, which showed the high efficiency to harvest two green microalgae in a low GO2 fermentation broth/algal culture ratio.http://link.springer.com/article/10.1186/s13068-017-0987-6Biomass-degrading bacteriumPseudomonas sp. GO2Corn stoverBioflocculantsMicroalgae harvest |
| spellingShingle | Haipeng Guo Chuntao Hong Bingsong Zheng Fan Lu Dean Jiang Wensheng Qin Bioflocculants’ production in a biomass-degrading bacterium using untreated corn stover as carbon source and use of bioflocculants for microalgae harvest Biotechnology for Biofuels Biomass-degrading bacterium Pseudomonas sp. GO2 Corn stover Bioflocculants Microalgae harvest |
| title | Bioflocculants’ production in a biomass-degrading bacterium using untreated corn stover as carbon source and use of bioflocculants for microalgae harvest |
| title_full | Bioflocculants’ production in a biomass-degrading bacterium using untreated corn stover as carbon source and use of bioflocculants for microalgae harvest |
| title_fullStr | Bioflocculants’ production in a biomass-degrading bacterium using untreated corn stover as carbon source and use of bioflocculants for microalgae harvest |
| title_full_unstemmed | Bioflocculants’ production in a biomass-degrading bacterium using untreated corn stover as carbon source and use of bioflocculants for microalgae harvest |
| title_short | Bioflocculants’ production in a biomass-degrading bacterium using untreated corn stover as carbon source and use of bioflocculants for microalgae harvest |
| title_sort | bioflocculants production in a biomass degrading bacterium using untreated corn stover as carbon source and use of bioflocculants for microalgae harvest |
| topic | Biomass-degrading bacterium Pseudomonas sp. GO2 Corn stover Bioflocculants Microalgae harvest |
| url | http://link.springer.com/article/10.1186/s13068-017-0987-6 |
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