Keberadaan Anisakis typica (Anisakidae) dari Ikan Tongkol dan Ikan Layang dari perairan Sulawesi Barat
Anisakis (L3-stage) is a parasitic nematode commonly found in marine fish or squid serve as intermediate or paratenic host. The purpose of this research was to identify Anisakis larvae using PCR-RFLP and sequencing methods. A total of 30 individuals of each species: bullet tuna (Auxis rochei) and In...
| Main Authors: | , |
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| Format: | Article |
| Language: | Indonesian |
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Universitas Gadjah Mada
2017-02-01
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| Series: | Jurnal Sain Veteriner |
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| Online Access: | https://jurnal.ugm.ac.id/jsv/article/view/22822 |
| _version_ | 1819012636504752128 |
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| author | Muhammad Dusil Hafid Hilal Anshary |
| author_facet | Muhammad Dusil Hafid Hilal Anshary |
| author_sort | Muhammad Dusil Hafid |
| collection | DOAJ |
| description | Anisakis (L3-stage) is a parasitic nematode commonly found in marine fish or squid serve as intermediate or paratenic host. The purpose of this research was to identify Anisakis larvae using PCR-RFLP and sequencing methods. A total of 30 individuals of each species: bullet tuna (Auxis rochei) and Indian scad
(Decapterus russeli) were examined for presence of Anisakis spp. Fish samples caught by fishermen around Mamuju waters were purchased at a fish market. The fish was necropsied and the organs: liver, intestine wall, heart and muscle were removed and put on separated petri dishes and examined for parasites under a stereo microscope. Anisakis found was cleaned and fixed in 70% alcohol. Initial identification was based on ventriculus shape and presence of mucron to distinguish Anisakis type I and Anisakis type II. All Anisakis found belong to Anisakis type I. Ten Anisakis type I were isolated, cleaned and stored in microfuge tubes containing 70% alcohol. The parasite gDNA was extracted using Wizard genomic DNA extraction and purification kit (Promega).
Species of Anisakis was determined by PCR-RFLP at ITS1-5.8S-ITS2 region using two restriction enzymes: Taq I and Hinf I and PCR-sequencing in mtDNA cox2 region. Analysis by PCR-RFLP showed that all Anisakis type I
examined was in the same pattern as Anisakis typica. Sequencing in mtDNAcox-2 region and the phylogenetic analysis showed that all samples were in the same cluster as A. typica. Based on PCR-RFLP and sequencing
analysis, all Anisakis found in this study belong to A. typica. |
| first_indexed | 2024-12-21T01:47:12Z |
| format | Article |
| id | doaj.art-6b07e4dbe991464f8e6468ea69978ca7 |
| institution | Directory Open Access Journal |
| issn | 0126-0421 2407-3733 |
| language | Indonesian |
| last_indexed | 2024-12-21T01:47:12Z |
| publishDate | 2017-02-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Jurnal Sain Veteriner |
| spelling | doaj.art-6b07e4dbe991464f8e6468ea69978ca72022-12-21T19:19:59ZindUniversitas Gadjah MadaJurnal Sain Veteriner0126-04212407-37332017-02-0134110211110.22146/jsv.2282215543Keberadaan Anisakis typica (Anisakidae) dari Ikan Tongkol dan Ikan Layang dari perairan Sulawesi BaratMuhammad Dusil Hafid0Hilal Anshary1Fish Quarantine and Inspection Agency, Fish quarantine station at West Sulawesi, Mamuju, IndonesiaLaboratory of Fish Parasites and Diseases, Faculty of Marine Science and Fisheries, Hasanuddin University, IndonesiaAnisakis (L3-stage) is a parasitic nematode commonly found in marine fish or squid serve as intermediate or paratenic host. The purpose of this research was to identify Anisakis larvae using PCR-RFLP and sequencing methods. A total of 30 individuals of each species: bullet tuna (Auxis rochei) and Indian scad (Decapterus russeli) were examined for presence of Anisakis spp. Fish samples caught by fishermen around Mamuju waters were purchased at a fish market. The fish was necropsied and the organs: liver, intestine wall, heart and muscle were removed and put on separated petri dishes and examined for parasites under a stereo microscope. Anisakis found was cleaned and fixed in 70% alcohol. Initial identification was based on ventriculus shape and presence of mucron to distinguish Anisakis type I and Anisakis type II. All Anisakis found belong to Anisakis type I. Ten Anisakis type I were isolated, cleaned and stored in microfuge tubes containing 70% alcohol. The parasite gDNA was extracted using Wizard genomic DNA extraction and purification kit (Promega). Species of Anisakis was determined by PCR-RFLP at ITS1-5.8S-ITS2 region using two restriction enzymes: Taq I and Hinf I and PCR-sequencing in mtDNA cox2 region. Analysis by PCR-RFLP showed that all Anisakis type I examined was in the same pattern as Anisakis typica. Sequencing in mtDNAcox-2 region and the phylogenetic analysis showed that all samples were in the same cluster as A. typica. Based on PCR-RFLP and sequencing analysis, all Anisakis found in this study belong to A. typica.https://jurnal.ugm.ac.id/jsv/article/view/22822Anisakismarine fishmtDNA cox-2PCR-RFLPSulawesi |
| spellingShingle | Muhammad Dusil Hafid Hilal Anshary Keberadaan Anisakis typica (Anisakidae) dari Ikan Tongkol dan Ikan Layang dari perairan Sulawesi Barat Jurnal Sain Veteriner Anisakis marine fish mtDNA cox-2 PCR-RFLP Sulawesi |
| title | Keberadaan Anisakis typica (Anisakidae) dari Ikan Tongkol dan Ikan Layang dari perairan Sulawesi Barat |
| title_full | Keberadaan Anisakis typica (Anisakidae) dari Ikan Tongkol dan Ikan Layang dari perairan Sulawesi Barat |
| title_fullStr | Keberadaan Anisakis typica (Anisakidae) dari Ikan Tongkol dan Ikan Layang dari perairan Sulawesi Barat |
| title_full_unstemmed | Keberadaan Anisakis typica (Anisakidae) dari Ikan Tongkol dan Ikan Layang dari perairan Sulawesi Barat |
| title_short | Keberadaan Anisakis typica (Anisakidae) dari Ikan Tongkol dan Ikan Layang dari perairan Sulawesi Barat |
| title_sort | keberadaan anisakis typica anisakidae dari ikan tongkol dan ikan layang dari perairan sulawesi barat |
| topic | Anisakis marine fish mtDNA cox-2 PCR-RFLP Sulawesi |
| url | https://jurnal.ugm.ac.id/jsv/article/view/22822 |
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