Detection of antimicrobial resistance genes in Lactobacillus spp. from poultry probiotic products and their horizontal transfer among Escherichia coli
The study was conducted to identify the antimicrobial resistance genes (ARGs) in Lactobacillus spp. from poultry probiotic products and their potential to spread among Escherichia coli. Lactobacillus spp. were isolated and identified from 35 poultry probiotic samples based on the cultural, biochemic...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Veterinary and Animal Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2451943X23000091 |
| _version_ | 1797818643604570112 |
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| author | Md. Rokon-Uz-Zaman Anica Bushra Tanjida Akter Pospo Monika Akter Runa Sadia Tasnuva Mst. Sonia Parvin Md. Taohidul Islam |
| author_facet | Md. Rokon-Uz-Zaman Anica Bushra Tanjida Akter Pospo Monika Akter Runa Sadia Tasnuva Mst. Sonia Parvin Md. Taohidul Islam |
| author_sort | Md. Rokon-Uz-Zaman |
| collection | DOAJ |
| description | The study was conducted to identify the antimicrobial resistance genes (ARGs) in Lactobacillus spp. from poultry probiotic products and their potential to spread among Escherichia coli. Lactobacillus spp. were isolated and identified from 35 poultry probiotic samples based on the cultural, biochemical, and molecular findings. All the isolates (n = 35) were screened for the presence of some ARGs such as β-lactamases encoding genes (blaTEM, blaCTXM-1, and blaCTXM-2), plasmid-mediated quinolone resistance gene (qnrA, qnrB, and qnrS), and tetracycline resistance genes (tetA and tetB). Five Lactobacillus spp. isolates from three brands were positive for one or more ARGs. The qnrS was detected in four isolates. The blaTEM and tetB were detected in two isolates. One isolate contained blaCTX-M-1, blaCTX-M-2, and tetA genes. Brand-wise analysis revealed that one isolate from Brand 4 contained blaTEM, blaCTX-M-1, blaCTX-M-2, qnrS, and tetA genes, one isolate from Brand 2 contained blaTEM gene, and three isolates from Brand 7 harbored qnrS gene. The co-culture of Lactobacillus spp. and E. coli resulted in the transmission of qnrS, CTX-M-1, and tetA from Lactobacillus spp. to E. coli. Results of antimicrobial susceptibility test revealed that the highest resistance was observed to cefepime and cefotaxime followed by penicillin G, oxacillin, cefuroxime, and ofloxacin. The findings of the present study indicate the potential risk of horizontal spread of antimicrobial resistance through probiotic bacteria among the poultry population. Therefore, it is very necessary to check for ARGs along with other attributes of probiotic bacteria to avoid the inclusion of resistant strains in probiotics. |
| first_indexed | 2024-03-13T09:10:59Z |
| format | Article |
| id | doaj.art-6b09bb5fb4fe4240b193a69d317d1e33 |
| institution | Directory Open Access Journal |
| issn | 2451-943X |
| language | English |
| last_indexed | 2024-03-13T09:10:59Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Veterinary and Animal Science |
| spelling | doaj.art-6b09bb5fb4fe4240b193a69d317d1e332023-05-27T04:26:16ZengElsevierVeterinary and Animal Science2451-943X2023-06-0120100292Detection of antimicrobial resistance genes in Lactobacillus spp. from poultry probiotic products and their horizontal transfer among Escherichia coliMd. Rokon-Uz-Zaman0Anica Bushra1Tanjida Akter Pospo2Monika Akter Runa3Sadia Tasnuva4Mst. Sonia Parvin5Md. Taohidul Islam6Population Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshPopulation Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshPopulation Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshPopulation Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshPopulation Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshPopulation Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshCorresponding author at: Population Medicine and AMR Laboratory, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.; Population Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshThe study was conducted to identify the antimicrobial resistance genes (ARGs) in Lactobacillus spp. from poultry probiotic products and their potential to spread among Escherichia coli. Lactobacillus spp. were isolated and identified from 35 poultry probiotic samples based on the cultural, biochemical, and molecular findings. All the isolates (n = 35) were screened for the presence of some ARGs such as β-lactamases encoding genes (blaTEM, blaCTXM-1, and blaCTXM-2), plasmid-mediated quinolone resistance gene (qnrA, qnrB, and qnrS), and tetracycline resistance genes (tetA and tetB). Five Lactobacillus spp. isolates from three brands were positive for one or more ARGs. The qnrS was detected in four isolates. The blaTEM and tetB were detected in two isolates. One isolate contained blaCTX-M-1, blaCTX-M-2, and tetA genes. Brand-wise analysis revealed that one isolate from Brand 4 contained blaTEM, blaCTX-M-1, blaCTX-M-2, qnrS, and tetA genes, one isolate from Brand 2 contained blaTEM gene, and three isolates from Brand 7 harbored qnrS gene. The co-culture of Lactobacillus spp. and E. coli resulted in the transmission of qnrS, CTX-M-1, and tetA from Lactobacillus spp. to E. coli. Results of antimicrobial susceptibility test revealed that the highest resistance was observed to cefepime and cefotaxime followed by penicillin G, oxacillin, cefuroxime, and ofloxacin. The findings of the present study indicate the potential risk of horizontal spread of antimicrobial resistance through probiotic bacteria among the poultry population. Therefore, it is very necessary to check for ARGs along with other attributes of probiotic bacteria to avoid the inclusion of resistant strains in probiotics.http://www.sciencedirect.com/science/article/pii/S2451943X23000091ProbioticsLactobacillusAntimicrobial resistance geneHorizontal transferPoultry |
| spellingShingle | Md. Rokon-Uz-Zaman Anica Bushra Tanjida Akter Pospo Monika Akter Runa Sadia Tasnuva Mst. Sonia Parvin Md. Taohidul Islam Detection of antimicrobial resistance genes in Lactobacillus spp. from poultry probiotic products and their horizontal transfer among Escherichia coli Veterinary and Animal Science Probiotics Lactobacillus Antimicrobial resistance gene Horizontal transfer Poultry |
| title | Detection of antimicrobial resistance genes in Lactobacillus spp. from poultry probiotic products and their horizontal transfer among Escherichia coli |
| title_full | Detection of antimicrobial resistance genes in Lactobacillus spp. from poultry probiotic products and their horizontal transfer among Escherichia coli |
| title_fullStr | Detection of antimicrobial resistance genes in Lactobacillus spp. from poultry probiotic products and their horizontal transfer among Escherichia coli |
| title_full_unstemmed | Detection of antimicrobial resistance genes in Lactobacillus spp. from poultry probiotic products and their horizontal transfer among Escherichia coli |
| title_short | Detection of antimicrobial resistance genes in Lactobacillus spp. from poultry probiotic products and their horizontal transfer among Escherichia coli |
| title_sort | detection of antimicrobial resistance genes in lactobacillus spp from poultry probiotic products and their horizontal transfer among escherichia coli |
| topic | Probiotics Lactobacillus Antimicrobial resistance gene Horizontal transfer Poultry |
| url | http://www.sciencedirect.com/science/article/pii/S2451943X23000091 |
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