A panel data set on harvest and perfusion decellularization of porcine rectus abdominis

In this dataset, we particularly depicted the harvest and perfusion decellularization of porcine rectus abdominis (RA), accompanied with displaying of the retained vascular trees within the perfusion-decellularized skeletal muscle matrix (pM-ECM) using vascular corrosion casting. In addition, severa...

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Main Authors: Jian Zhang, Wen Yue Cheng, Zhi Qian Hu, Neill J. Turner, Li Zhang, Qiang Wang, Stephen F. Badylak
Format: Article
Language:English
Published: Elsevier 2016-06-01
Series:Data in Brief
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340916302323
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author Jian Zhang
Wen Yue Cheng
Zhi Qian Hu
Neill J. Turner
Li Zhang
Qiang Wang
Stephen F. Badylak
author_facet Jian Zhang
Wen Yue Cheng
Zhi Qian Hu
Neill J. Turner
Li Zhang
Qiang Wang
Stephen F. Badylak
author_sort Jian Zhang
collection DOAJ
description In this dataset, we particularly depicted the harvest and perfusion decellularization of porcine rectus abdominis (RA), accompanied with displaying of the retained vascular trees within the perfusion-decellularized skeletal muscle matrix (pM-ECM) using vascular corrosion casting. In addition, several important tips for successful pM-ECM preparation were emphasized, which including using anatomically isolated skeletal muscle as tissue source with all main feeding and draining vessels perfused, preserving the internal microcirculation availability, aseptic technique and pyrogen free in all steps, sequential perfusion via artery or vein, and longtime washing after decellularization. The data are supplemental to our original research article describing detailed associations of pM-ECM as a clinically relevant scale, three-dimensional scaffold with a vascular network template for tissue-specific regeneration, “Perfusion-decellularized skeletal muscle as a three-dimensional scaffold with a vascular network template” Zhang et al. (2016) [1].
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spelling doaj.art-6b0bce97996d478ea5af6186994748132022-12-21T18:57:10ZengElsevierData in Brief2352-34092016-06-01713751382A panel data set on harvest and perfusion decellularization of porcine rectus abdominisJian Zhang0Wen Yue Cheng1Zhi Qian Hu2Neill J. Turner3Li Zhang4Qiang Wang5Stephen F. Badylak6Department of Surgery, Shanghai Chang Zheng Hospital, Second Military Medical University, Shanghai 200003, PR China; Department of Regenerative Medicine, Shanghai Zhabei District Central Hospital, Shanghai 200072, PR ChinaDepartment of Regenerative Medicine, Shanghai Zhabei District Central Hospital, Shanghai 200072, PR ChinaDepartment of Surgery, Shanghai Chang Zheng Hospital, Second Military Medical University, Shanghai 200003, PR ChinaMcGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USAMcGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USADepartment of Surgery, Shanghai Chang Zheng Hospital, Second Military Medical University, Shanghai 200003, PR China; Department of Regenerative Medicine, Shanghai Zhabei District Central Hospital, Shanghai 200072, PR China; Department of General Surgery, Shanghai Zhabei District Central Hospital, Shanghai 200072, PR China; Corresponding author at: Department of Surgery, Shanghai Chang Zheng Hospital, Second Military Medical University, 415 Feng Yang Road, Shanghai 200003, PR China. Tel.: + 86 21 5662 8584; fax: + 86 21 56316699.McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USA; Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15129, USA; Corresponding author at: University of Pittsburgh, McGowan Institute for Regenerative Medicine, 450 Technology Drive Suite 300, Pittsburgh, PA 15219, USA. Tel.: + 1 412 624 5253; fax: + 1 412 624 5256.In this dataset, we particularly depicted the harvest and perfusion decellularization of porcine rectus abdominis (RA), accompanied with displaying of the retained vascular trees within the perfusion-decellularized skeletal muscle matrix (pM-ECM) using vascular corrosion casting. In addition, several important tips for successful pM-ECM preparation were emphasized, which including using anatomically isolated skeletal muscle as tissue source with all main feeding and draining vessels perfused, preserving the internal microcirculation availability, aseptic technique and pyrogen free in all steps, sequential perfusion via artery or vein, and longtime washing after decellularization. The data are supplemental to our original research article describing detailed associations of pM-ECM as a clinically relevant scale, three-dimensional scaffold with a vascular network template for tissue-specific regeneration, “Perfusion-decellularized skeletal muscle as a three-dimensional scaffold with a vascular network template” Zhang et al. (2016) [1].http://www.sciencedirect.com/science/article/pii/S2352340916302323
spellingShingle Jian Zhang
Wen Yue Cheng
Zhi Qian Hu
Neill J. Turner
Li Zhang
Qiang Wang
Stephen F. Badylak
A panel data set on harvest and perfusion decellularization of porcine rectus abdominis
Data in Brief
title A panel data set on harvest and perfusion decellularization of porcine rectus abdominis
title_full A panel data set on harvest and perfusion decellularization of porcine rectus abdominis
title_fullStr A panel data set on harvest and perfusion decellularization of porcine rectus abdominis
title_full_unstemmed A panel data set on harvest and perfusion decellularization of porcine rectus abdominis
title_short A panel data set on harvest and perfusion decellularization of porcine rectus abdominis
title_sort panel data set on harvest and perfusion decellularization of porcine rectus abdominis
url http://www.sciencedirect.com/science/article/pii/S2352340916302323
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