Pulmonary Microbial Composition in Sepsis-Induced Acute Respiratory Distress Syndrome
Background: Acute respiratory distress syndrome (ARDS) is an unresolved challenge in the field of respiratory and critical care, and the changes in the lung microbiome during the development of ARDS and their clinical diagnostic value remain unclear. This study aimed to explore the role of the lung...
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2022.862570/full |
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| author | Peng Zhang Baoyi Liu Weihao Zheng Yantang Chen Zhentao Wu Yuting Lu Jie Ma Wenjie Lu Mingzhu Zheng Wanting Wu Zijie Meng Jinhua Wu Yan Zheng Xin Zhang Xin Zhang Xin Zhang Shuang Zhang Yanming Huang Yanming Huang |
| author_facet | Peng Zhang Baoyi Liu Weihao Zheng Yantang Chen Zhentao Wu Yuting Lu Jie Ma Wenjie Lu Mingzhu Zheng Wanting Wu Zijie Meng Jinhua Wu Yan Zheng Xin Zhang Xin Zhang Xin Zhang Shuang Zhang Yanming Huang Yanming Huang |
| author_sort | Peng Zhang |
| collection | DOAJ |
| description | Background: Acute respiratory distress syndrome (ARDS) is an unresolved challenge in the field of respiratory and critical care, and the changes in the lung microbiome during the development of ARDS and their clinical diagnostic value remain unclear. This study aimed to explore the role of the lung microbiome in disease progression in patients with sepsis-induced ARDS and potential therapeutic targets.Methods: Patients with ARDS were divided into two groups according to the initial site of infection, intrapulmonary infection (ARDSp, 111 cases) and extrapulmonary infection (ARDSexp, 45 cases), and a total of 28 patients with mild pulmonary infections were enrolled as the control group. In this study, we sequenced the DNA in the bronchoalveolar lavage fluid collected from patients using metagenomic next-generation sequencing (mNGS) to analyze the changes in the lung microbiome in patients with different infectious site and prognosis and before and after antibiotic treatment.Results: The Shannon–Wiener index indicated a statistically significant reduction in microbial diversity in the ARDSp group compared with the ARDSexp and control groups. The ARDSp group was characterized by a reduction in microbiome diversity, mainly in the normal microbes of the lung, whereas the ARDSexp group was characterized by an increase in microbiome diversity, mainly in conditionally pathogenic bacteria and intestinal microbes. Further analysis showed that an increase in Bilophila is a potential risk factor for death in ARDSexp. An increase in Escherichia coli, Staphylococcus aureus, Candida albicans, enteric microbes, or conditional pathogens may be risk factors for death in ARDSp. In contrast, Hydrobacter may be a protective factor in ARDSp.Conclusion: Different initial sites of infection and prognoses are likely to affect the composition and diversity of the pulmonary microbiome in patients with septic ARDS. This study provides insights into disease development and exploration of potential therapeutic targets. |
| first_indexed | 2024-04-12T13:35:30Z |
| format | Article |
| id | doaj.art-6b1050b29ffa44d19ff449e8d96f0297 |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-04-12T13:35:30Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-6b1050b29ffa44d19ff449e8d96f02972022-12-22T03:31:01ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-06-01910.3389/fmolb.2022.862570862570Pulmonary Microbial Composition in Sepsis-Induced Acute Respiratory Distress SyndromePeng Zhang0Baoyi Liu1Weihao Zheng2Yantang Chen3Zhentao Wu4Yuting Lu5Jie Ma6Wenjie Lu7Mingzhu Zheng8Wanting Wu9Zijie Meng10Jinhua Wu11Yan Zheng12Xin Zhang13Xin Zhang14Xin Zhang15Shuang Zhang16Yanming Huang17Yanming Huang18Department of Critical Care Medicine, Jiangmen Central Hospital, Jiangmen, ChinaClinical Experimental Center, Jiangmen Key Laboratory of Clinical Biobanks and Translational Research, Jiangmen Central Hospital, Jiangmen, ChinaDepartment of Critical Care Medicine, Jiangmen Central Hospital, Jiangmen, ChinaDepartment of Critical Care Medicine, Jiangmen Central Hospital, Jiangmen, ChinaDepartment of Critical Care Medicine, Jiangmen Central Hospital, Jiangmen, ChinaDepartment of Critical Care Medicine, Jiangmen Central Hospital, Jiangmen, ChinaDepartment of Critical Care Medicine, Jiangmen Central Hospital, Jiangmen, ChinaClinical Experimental Center, Jiangmen Key Laboratory of Clinical Biobanks and Translational Research, Jiangmen Central Hospital, Jiangmen, ChinaClinical Experimental Center, Jiangmen Key Laboratory of Clinical Biobanks and Translational Research, Jiangmen Central Hospital, Jiangmen, ChinaClinical Experimental Center, Jiangmen Key Laboratory of Clinical Biobanks and Translational Research, Jiangmen Central Hospital, Jiangmen, ChinaClinical Experimental Center, Jiangmen Key Laboratory of Clinical Biobanks and Translational Research, Jiangmen Central Hospital, Jiangmen, ChinaDepartment of Clinical Laboratory, Jiangmen Central Hospital, Jiangmen, ChinaDepartment of Research and Development, Guangdong Research Institute of Genetic Diagnostic and Engineering Technologies for Thalassemia, Hybribio Limited, Guangzhou, ChinaClinical Experimental Center, Jiangmen Key Laboratory of Clinical Biobanks and Translational Research, Jiangmen Central Hospital, Jiangmen, ChinaDongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational Research, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, Dongguan, ChinaCollaborative Innovation Center for Antitumor Active Substance Research and Development, Guangdong Medical University, Zhanjiang, ChinaDepartment of Critical Care Medicine, Jiangmen Central Hospital, Jiangmen, ChinaClinical Experimental Center, Jiangmen Key Laboratory of Clinical Biobanks and Translational Research, Jiangmen Central Hospital, Jiangmen, ChinaDepartment of Respiratory and Critical Care Medicine, Jiangmen Central Hospital, Jiangmen, ChinaBackground: Acute respiratory distress syndrome (ARDS) is an unresolved challenge in the field of respiratory and critical care, and the changes in the lung microbiome during the development of ARDS and their clinical diagnostic value remain unclear. This study aimed to explore the role of the lung microbiome in disease progression in patients with sepsis-induced ARDS and potential therapeutic targets.Methods: Patients with ARDS were divided into two groups according to the initial site of infection, intrapulmonary infection (ARDSp, 111 cases) and extrapulmonary infection (ARDSexp, 45 cases), and a total of 28 patients with mild pulmonary infections were enrolled as the control group. In this study, we sequenced the DNA in the bronchoalveolar lavage fluid collected from patients using metagenomic next-generation sequencing (mNGS) to analyze the changes in the lung microbiome in patients with different infectious site and prognosis and before and after antibiotic treatment.Results: The Shannon–Wiener index indicated a statistically significant reduction in microbial diversity in the ARDSp group compared with the ARDSexp and control groups. The ARDSp group was characterized by a reduction in microbiome diversity, mainly in the normal microbes of the lung, whereas the ARDSexp group was characterized by an increase in microbiome diversity, mainly in conditionally pathogenic bacteria and intestinal microbes. Further analysis showed that an increase in Bilophila is a potential risk factor for death in ARDSexp. An increase in Escherichia coli, Staphylococcus aureus, Candida albicans, enteric microbes, or conditional pathogens may be risk factors for death in ARDSp. In contrast, Hydrobacter may be a protective factor in ARDSp.Conclusion: Different initial sites of infection and prognoses are likely to affect the composition and diversity of the pulmonary microbiome in patients with septic ARDS. This study provides insights into disease development and exploration of potential therapeutic targets.https://www.frontiersin.org/articles/10.3389/fmolb.2022.862570/fullacute respiratory distress syndrome(ARDS)sepsismetagenomic next-generation sequencing(mNGS)pulmonary microbiomeacute respiratory distress syndrome of pulmonaryacute respiratory distress syndrome of extrapulmonary |
| spellingShingle | Peng Zhang Baoyi Liu Weihao Zheng Yantang Chen Zhentao Wu Yuting Lu Jie Ma Wenjie Lu Mingzhu Zheng Wanting Wu Zijie Meng Jinhua Wu Yan Zheng Xin Zhang Xin Zhang Xin Zhang Shuang Zhang Yanming Huang Yanming Huang Pulmonary Microbial Composition in Sepsis-Induced Acute Respiratory Distress Syndrome Frontiers in Molecular Biosciences acute respiratory distress syndrome(ARDS) sepsis metagenomic next-generation sequencing(mNGS) pulmonary microbiome acute respiratory distress syndrome of pulmonary acute respiratory distress syndrome of extrapulmonary |
| title | Pulmonary Microbial Composition in Sepsis-Induced Acute Respiratory Distress Syndrome |
| title_full | Pulmonary Microbial Composition in Sepsis-Induced Acute Respiratory Distress Syndrome |
| title_fullStr | Pulmonary Microbial Composition in Sepsis-Induced Acute Respiratory Distress Syndrome |
| title_full_unstemmed | Pulmonary Microbial Composition in Sepsis-Induced Acute Respiratory Distress Syndrome |
| title_short | Pulmonary Microbial Composition in Sepsis-Induced Acute Respiratory Distress Syndrome |
| title_sort | pulmonary microbial composition in sepsis induced acute respiratory distress syndrome |
| topic | acute respiratory distress syndrome(ARDS) sepsis metagenomic next-generation sequencing(mNGS) pulmonary microbiome acute respiratory distress syndrome of pulmonary acute respiratory distress syndrome of extrapulmonary |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2022.862570/full |
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