Mesenchymal stem cells that located in the electromagnetic fields improves rat model of Parkinson's disease
Objective(s): The main characteristic of mesenchymal stem cells (MSCs) is their ability to produce other cell types. Electromagnetic field (EMF) stimulates differentiation of MSCs into other cells. In this study, we investigated whether EMF can effect on the differentiation of MSCs into dopaminergic...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2016-07-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_7358_2eae75e9bb96b343a7bf8de4579c873c.pdf |
| _version_ | 1818824304830185472 |
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| author | Majid Jadidi Saeed Moghadas Biat Hamid Reza Sameni Manouchehr Safari Abbas Ali Vafaei Laya Ghahari |
| author_facet | Majid Jadidi Saeed Moghadas Biat Hamid Reza Sameni Manouchehr Safari Abbas Ali Vafaei Laya Ghahari |
| author_sort | Majid Jadidi |
| collection | DOAJ |
| description | Objective(s): The main characteristic of mesenchymal stem cells (MSCs) is their ability to produce other cell types. Electromagnetic field (EMF) stimulates differentiation of MSCs into other cells. In this study, we investigated whether EMF can effect on the differentiation of MSCs into dopaminergic (DA) neurons. Materials and Methods: An EMF with a frequency of 50 Hz and two intensities of 40 and 400 µT 1hr/day was generated around the cells for a week. Afterwards, these cells were injected into the left ventricle of Parkinsonian rats. The rats survived for 2 weeks, and then sampling was performed. Results: The injected cells differentiated into DA neurons and sporadically settled in the substantia nigra pars compacta (SNpc). Transplanted rats exhibited significant partial correction apomorphine-induced rotational behavior compared to Parkinsonian rats (5.0±0.1 vs 7.57±0.08). Results demonstrated that endogenous serum and brain derived neurotrophic factor (BDNF) were altered in all experimental groups. The greatest increase was in group of 400 µT EMF in comparison with Parkinsonian rats (398±15 vs. 312±11.79 pg ⁄ mg). Current study have shown that 6-Hydroxydopamine can cause severe loss of dopaminergic neurons (68±6.58), but injected MSCs that exposed to 40 and 400 µT EMF increased dopaminergic neurons in SNpc ( 108±2.33 & 126±3.89) (P |
| first_indexed | 2024-12-18T23:53:45Z |
| format | Article |
| id | doaj.art-6b12b9efae80496fb4381e998ef15940 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-18T23:53:45Z |
| publishDate | 2016-07-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-6b12b9efae80496fb4381e998ef159402022-12-21T20:46:49ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742016-07-011977417487358Mesenchymal stem cells that located in the electromagnetic fields improves rat model of Parkinson's diseaseMajid Jadidi0Saeed Moghadas Biat1Hamid Reza Sameni2Manouchehr Safari3Abbas Ali Vafaei4Laya Ghahari5Department of Medical Physics, School of Medicine, Semnan University of Medical Sciences, Semnan, IranResearch Center of Nervous System Stem Cells, Department of Anatomy, School of Medicine, Semnan University of Medical Sciences, Semnan, IranResearch Center of Nervous System Stem Cells, Department of Anatomy, School of Medicine, Semnan University of Medical Sciences, Semnan, IranResearch Center of Nervous System Stem Cells, Department of Anatomy, School of Medicine, Semnan University of Medical Sciences, Semnan, IranDepartment of Physiology, School of Medicine, Semnan University of Medical Sciences, Semnan, IranDepartment of Anatomy, School of Medicine, AJA Medical University, Tehran, IranObjective(s): The main characteristic of mesenchymal stem cells (MSCs) is their ability to produce other cell types. Electromagnetic field (EMF) stimulates differentiation of MSCs into other cells. In this study, we investigated whether EMF can effect on the differentiation of MSCs into dopaminergic (DA) neurons. Materials and Methods: An EMF with a frequency of 50 Hz and two intensities of 40 and 400 µT 1hr/day was generated around the cells for a week. Afterwards, these cells were injected into the left ventricle of Parkinsonian rats. The rats survived for 2 weeks, and then sampling was performed. Results: The injected cells differentiated into DA neurons and sporadically settled in the substantia nigra pars compacta (SNpc). Transplanted rats exhibited significant partial correction apomorphine-induced rotational behavior compared to Parkinsonian rats (5.0±0.1 vs 7.57±0.08). Results demonstrated that endogenous serum and brain derived neurotrophic factor (BDNF) were altered in all experimental groups. The greatest increase was in group of 400 µT EMF in comparison with Parkinsonian rats (398±15 vs. 312±11.79 pg ⁄ mg). Current study have shown that 6-Hydroxydopamine can cause severe loss of dopaminergic neurons (68±6.58), but injected MSCs that exposed to 40 and 400 µT EMF increased dopaminergic neurons in SNpc ( 108±2.33 & 126±3.89) (Phttp://ijbms.mums.ac.ir/article_7358_2eae75e9bb96b343a7bf8de4579c873c.pdfDopaminergic neuronElectromagnetic fieldMesenchymal stem cellsParkinson’s disease |
| spellingShingle | Majid Jadidi Saeed Moghadas Biat Hamid Reza Sameni Manouchehr Safari Abbas Ali Vafaei Laya Ghahari Mesenchymal stem cells that located in the electromagnetic fields improves rat model of Parkinson's disease Iranian Journal of Basic Medical Sciences Dopaminergic neuron Electromagnetic field Mesenchymal stem cells Parkinson’s disease |
| title | Mesenchymal stem cells that located in the electromagnetic fields improves rat model of Parkinson's disease |
| title_full | Mesenchymal stem cells that located in the electromagnetic fields improves rat model of Parkinson's disease |
| title_fullStr | Mesenchymal stem cells that located in the electromagnetic fields improves rat model of Parkinson's disease |
| title_full_unstemmed | Mesenchymal stem cells that located in the electromagnetic fields improves rat model of Parkinson's disease |
| title_short | Mesenchymal stem cells that located in the electromagnetic fields improves rat model of Parkinson's disease |
| title_sort | mesenchymal stem cells that located in the electromagnetic fields improves rat model of parkinson s disease |
| topic | Dopaminergic neuron Electromagnetic field Mesenchymal stem cells Parkinson’s disease |
| url | http://ijbms.mums.ac.ir/article_7358_2eae75e9bb96b343a7bf8de4579c873c.pdf |
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