Genome-Wide Identification and Expression Analysis of GATA Gene Family under Different Nitrogen Levels in <i>Arachis hypogaea</i> L.
Nitrogen, one of the essential elements, is a key determinant for improving peanut growth and yield. GATA zinc finger transcription factors have been found to be involved in regulation of nitrogen metabolism. However, a systematic characterization of the GATA gene family and patterns of their expres...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/1/215 |
| _version_ | 1797447110431342592 |
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| author | Xiujie Li Xiaoxu Deng Suoyi Han Xinyou Zhang Tingbo Dai |
| author_facet | Xiujie Li Xiaoxu Deng Suoyi Han Xinyou Zhang Tingbo Dai |
| author_sort | Xiujie Li |
| collection | DOAJ |
| description | Nitrogen, one of the essential elements, is a key determinant for improving peanut growth and yield. GATA zinc finger transcription factors have been found to be involved in regulation of nitrogen metabolism. However, a systematic characterization of the GATA gene family and patterns of their expression under different nitrogen levels remains elusive. In this study, a total of 45 GATA genes distributed among 17 chromosomes were identified in the peanut genome and classified into three subfamilies I, II and III with 26, 13 and 6 members, respectively, whose physicochemical characteristics, gene structures and conserved motifs were also analyzed. Furthermore, the optimal level of nitrogen fertilizer on the growth of peanut cultivar Yuhua 23 was determined by pod yield and value cost ratio from 2020 to 2022, and the results revealed that 150 kg hm<sup>−2</sup> nitrogen was the best for cultivation of peanut Yuhua 23 because of its highest pod yield and relatively higher VCR of more than four. In addition, expression patterns of peanut GATA genes under different nitrogen levels were detected by real-time quantitative PCR and several GATA genes were significantly changed under a nitrogen level of 150 kg hm<sup>−2</sup>. Overall, the above results would be helpful for further understanding biological functions of the GATA gene family in cultivated peanut. |
| first_indexed | 2024-03-09T13:51:07Z |
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| id | doaj.art-6b14343b1f654429b235a0d91a8ef9e9 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T13:51:07Z |
| publishDate | 2023-01-01 |
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| series | Agronomy |
| spelling | doaj.art-6b14343b1f654429b235a0d91a8ef9e92023-11-30T20:50:37ZengMDPI AGAgronomy2073-43952023-01-0113121510.3390/agronomy13010215Genome-Wide Identification and Expression Analysis of GATA Gene Family under Different Nitrogen Levels in <i>Arachis hypogaea</i> L.Xiujie Li0Xiaoxu Deng1Suoyi Han2Xinyou Zhang3Tingbo Dai4College of Agriculture, Nanjing Agricultural University, Nanjing 210095, ChinaHenan Academy of Agricultural Sciences, Zhengzhou 450002, ChinaHenan Academy of Agricultural Sciences, Zhengzhou 450002, ChinaHenan Academy of Agricultural Sciences, Zhengzhou 450002, ChinaCollege of Agriculture, Nanjing Agricultural University, Nanjing 210095, ChinaNitrogen, one of the essential elements, is a key determinant for improving peanut growth and yield. GATA zinc finger transcription factors have been found to be involved in regulation of nitrogen metabolism. However, a systematic characterization of the GATA gene family and patterns of their expression under different nitrogen levels remains elusive. In this study, a total of 45 GATA genes distributed among 17 chromosomes were identified in the peanut genome and classified into three subfamilies I, II and III with 26, 13 and 6 members, respectively, whose physicochemical characteristics, gene structures and conserved motifs were also analyzed. Furthermore, the optimal level of nitrogen fertilizer on the growth of peanut cultivar Yuhua 23 was determined by pod yield and value cost ratio from 2020 to 2022, and the results revealed that 150 kg hm<sup>−2</sup> nitrogen was the best for cultivation of peanut Yuhua 23 because of its highest pod yield and relatively higher VCR of more than four. In addition, expression patterns of peanut GATA genes under different nitrogen levels were detected by real-time quantitative PCR and several GATA genes were significantly changed under a nitrogen level of 150 kg hm<sup>−2</sup>. Overall, the above results would be helpful for further understanding biological functions of the GATA gene family in cultivated peanut.https://www.mdpi.com/2073-4395/13/1/215peanutGATAgene familynitrogen levelexpression analysis |
| spellingShingle | Xiujie Li Xiaoxu Deng Suoyi Han Xinyou Zhang Tingbo Dai Genome-Wide Identification and Expression Analysis of GATA Gene Family under Different Nitrogen Levels in <i>Arachis hypogaea</i> L. Agronomy peanut GATA gene family nitrogen level expression analysis |
| title | Genome-Wide Identification and Expression Analysis of GATA Gene Family under Different Nitrogen Levels in <i>Arachis hypogaea</i> L. |
| title_full | Genome-Wide Identification and Expression Analysis of GATA Gene Family under Different Nitrogen Levels in <i>Arachis hypogaea</i> L. |
| title_fullStr | Genome-Wide Identification and Expression Analysis of GATA Gene Family under Different Nitrogen Levels in <i>Arachis hypogaea</i> L. |
| title_full_unstemmed | Genome-Wide Identification and Expression Analysis of GATA Gene Family under Different Nitrogen Levels in <i>Arachis hypogaea</i> L. |
| title_short | Genome-Wide Identification and Expression Analysis of GATA Gene Family under Different Nitrogen Levels in <i>Arachis hypogaea</i> L. |
| title_sort | genome wide identification and expression analysis of gata gene family under different nitrogen levels in i arachis hypogaea i l |
| topic | peanut GATA gene family nitrogen level expression analysis |
| url | https://www.mdpi.com/2073-4395/13/1/215 |
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