Preparation of Oil Palm Empty Fruit Bunch Hydrolysate
Malaysia is the second largest palm oil producer and exporter globally. When crude palm oil is produced in both plantations and oil processing mills, a large amount of oil palm empty fruit bunch (OPEFB) is simultaneously produced as a waste product. Here, we describe the preparation of hydrolysate f...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/7/2/81 |
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| author | Hironaga Akita Mohd Zulkhairi Mohd Yusoff Shinji Fujimoto |
| author_facet | Hironaga Akita Mohd Zulkhairi Mohd Yusoff Shinji Fujimoto |
| author_sort | Hironaga Akita |
| collection | DOAJ |
| description | Malaysia is the second largest palm oil producer and exporter globally. When crude palm oil is produced in both plantations and oil processing mills, a large amount of oil palm empty fruit bunch (OPEFB) is simultaneously produced as a waste product. Here, we describe the preparation of hydrolysate from OPEFB. After OPEFB was hydrothermally treated at 180–200 °C, the resultant liquid phase was subjected to high-performance liquid chromatography analysis, while the solid phase was used for acidic and enzymatic hydrolysis. Hemicellulose yield from the acid-treated solid phase decreased from 153 mg/g-OPEFB to 27.5 mg/g-OPEFB by increasing the hydrothermal treatment temperature from 180 to 200 °C. Glucose yield from the enzyme-treated solid phase obtained after hydrothermal treatment at 200 °C was the highest (234 ± 1.90 mg/g-OPEFB, 61.7% production efficiency). In contrast, xylose, mannose, galactose, and arabinose yields in the hydrolysate prepared from the solid phase hydrothermally treated at 200 °C were the lowest. Thus, we concluded that the optimum temperature for hydrothermal pretreatment was 200 °C, which was caused by the low hemicellulose yield. Based on these results, we have established an effective method for preparing OPEFB hydrolysates with high glucose content. |
| first_indexed | 2024-03-10T11:03:27Z |
| format | Article |
| id | doaj.art-6b2025a985b84c6a9c9286ca60c34087 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T11:03:27Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-6b2025a985b84c6a9c9286ca60c340872023-11-21T21:17:52ZengMDPI AGFermentation2311-56372021-05-01728110.3390/fermentation7020081Preparation of Oil Palm Empty Fruit Bunch HydrolysateHironaga Akita0Mohd Zulkhairi Mohd Yusoff1Shinji Fujimoto2Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, JapanDepartment of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Putra Malaysia University, Serdang 43400, Selangor, MalaysiaResearch Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, JapanMalaysia is the second largest palm oil producer and exporter globally. When crude palm oil is produced in both plantations and oil processing mills, a large amount of oil palm empty fruit bunch (OPEFB) is simultaneously produced as a waste product. Here, we describe the preparation of hydrolysate from OPEFB. After OPEFB was hydrothermally treated at 180–200 °C, the resultant liquid phase was subjected to high-performance liquid chromatography analysis, while the solid phase was used for acidic and enzymatic hydrolysis. Hemicellulose yield from the acid-treated solid phase decreased from 153 mg/g-OPEFB to 27.5 mg/g-OPEFB by increasing the hydrothermal treatment temperature from 180 to 200 °C. Glucose yield from the enzyme-treated solid phase obtained after hydrothermal treatment at 200 °C was the highest (234 ± 1.90 mg/g-OPEFB, 61.7% production efficiency). In contrast, xylose, mannose, galactose, and arabinose yields in the hydrolysate prepared from the solid phase hydrothermally treated at 200 °C were the lowest. Thus, we concluded that the optimum temperature for hydrothermal pretreatment was 200 °C, which was caused by the low hemicellulose yield. Based on these results, we have established an effective method for preparing OPEFB hydrolysates with high glucose content.https://www.mdpi.com/2311-5637/7/2/81oil palm empty fruit bunchhydrolysatehydrothermal pretreatmentacid hydrolysisenzymatic hydrolysishemicellulose |
| spellingShingle | Hironaga Akita Mohd Zulkhairi Mohd Yusoff Shinji Fujimoto Preparation of Oil Palm Empty Fruit Bunch Hydrolysate Fermentation oil palm empty fruit bunch hydrolysate hydrothermal pretreatment acid hydrolysis enzymatic hydrolysis hemicellulose |
| title | Preparation of Oil Palm Empty Fruit Bunch Hydrolysate |
| title_full | Preparation of Oil Palm Empty Fruit Bunch Hydrolysate |
| title_fullStr | Preparation of Oil Palm Empty Fruit Bunch Hydrolysate |
| title_full_unstemmed | Preparation of Oil Palm Empty Fruit Bunch Hydrolysate |
| title_short | Preparation of Oil Palm Empty Fruit Bunch Hydrolysate |
| title_sort | preparation of oil palm empty fruit bunch hydrolysate |
| topic | oil palm empty fruit bunch hydrolysate hydrothermal pretreatment acid hydrolysis enzymatic hydrolysis hemicellulose |
| url | https://www.mdpi.com/2311-5637/7/2/81 |
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