MiR-140 downregulates fatty acid synthesis by targeting transforming growth factor alpha (TGFA) in bovine mammary epithelial cells
Fat is an indispensable nutrient and basic metabolite for sustaining life, and milk is particularly rich in fatty acids, including a variety of saturated and unsaturated fatty acids. MicroRNA (miRNA) and mRNA play an important role in the regulation of milk fat metabolism in mammary gland tissue. It...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S209531192200048X |
| _version_ | 1811208682749296640 |
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| author | Shuang-feng CHU Tian-qi ZHAO Abdelaziz Adam Idriss ARBAB Yi YANG Zhi CHEN Zhang-ping YANG |
| author_facet | Shuang-feng CHU Tian-qi ZHAO Abdelaziz Adam Idriss ARBAB Yi YANG Zhi CHEN Zhang-ping YANG |
| author_sort | Shuang-feng CHU |
| collection | DOAJ |
| description | Fat is an indispensable nutrient and basic metabolite for sustaining life, and milk is particularly rich in fatty acids, including a variety of saturated and unsaturated fatty acids. MicroRNA (miRNA) and mRNA play an important role in the regulation of milk fat metabolism in mammary gland tissue. It has been shown that lipid metabolism has a complex transcriptional regulation, but the mechanism by which milk fat synthesis is regulated through miRNA–mRNA interactions is poorly understood. In this study, we performed transcriptome sequencing with bovine mammary gland tissue in the late lactation (270 and 315 days after parturition) to identify the key gene that regulating milk fat metabolism. A total of 1 207 differentially coexpressed genes were selected, 828 upregulated genes and 379 downregulated genes were identified. The transforming growth factor alpha (TGFA) gene was selected as the target gene, and luciferase reporter assay, Western blotting and qRT-PCR were used for further study. The results demonstrated that miR-140 was an upstream regulator of TGFA, and miR-140 could inhibit (P<0.01) unsaturated fatty acid and triglyceride (TAGs) production in bovine mammary epithelial cells (BMECs). In contrast, TGFA promoted (P<0.01) unsaturated fatty acid and TAG production. Rescue experiments further indicated the miR-140/TGFA regulatory mechanism. Taken together, these results suggest that the miR-140/TGFA pathway can inhibit (P<0.01) milk fat metabolism and improve milk quality by genetic means. |
| first_indexed | 2024-04-12T04:26:46Z |
| format | Article |
| id | doaj.art-6b251e097c0b4f2eb8d820c53a4c2a7e |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-04-12T04:26:46Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-6b251e097c0b4f2eb8d820c53a4c2a7e2022-12-22T03:48:04ZengElsevierJournal of Integrative Agriculture2095-31192022-10-01211030043016MiR-140 downregulates fatty acid synthesis by targeting transforming growth factor alpha (TGFA) in bovine mammary epithelial cellsShuang-feng CHU0Tian-qi ZHAO1Abdelaziz Adam Idriss ARBAB2Yi YANG3Zhi CHEN4Zhang-ping YANG5College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, P.R.China; Correspondence YANG Zhang-ping, Tel: +86-514-87979307College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, P.R.ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, P.R.ChinaJoint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, P.R.China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, P.R.ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, P.R.China; Correspondence CHEN ZhiCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, P.R.China; Correspondence YANG Zhang-ping, Tel: +86-514-87979307Fat is an indispensable nutrient and basic metabolite for sustaining life, and milk is particularly rich in fatty acids, including a variety of saturated and unsaturated fatty acids. MicroRNA (miRNA) and mRNA play an important role in the regulation of milk fat metabolism in mammary gland tissue. It has been shown that lipid metabolism has a complex transcriptional regulation, but the mechanism by which milk fat synthesis is regulated through miRNA–mRNA interactions is poorly understood. In this study, we performed transcriptome sequencing with bovine mammary gland tissue in the late lactation (270 and 315 days after parturition) to identify the key gene that regulating milk fat metabolism. A total of 1 207 differentially coexpressed genes were selected, 828 upregulated genes and 379 downregulated genes were identified. The transforming growth factor alpha (TGFA) gene was selected as the target gene, and luciferase reporter assay, Western blotting and qRT-PCR were used for further study. The results demonstrated that miR-140 was an upstream regulator of TGFA, and miR-140 could inhibit (P<0.01) unsaturated fatty acid and triglyceride (TAGs) production in bovine mammary epithelial cells (BMECs). In contrast, TGFA promoted (P<0.01) unsaturated fatty acid and TAG production. Rescue experiments further indicated the miR-140/TGFA regulatory mechanism. Taken together, these results suggest that the miR-140/TGFA pathway can inhibit (P<0.01) milk fat metabolism and improve milk quality by genetic means.http://www.sciencedirect.com/science/article/pii/S209531192200048Xbovine mammary epithelial cellsTGFAtranscriptome sequencingmiR-140fatty acid metabolism |
| spellingShingle | Shuang-feng CHU Tian-qi ZHAO Abdelaziz Adam Idriss ARBAB Yi YANG Zhi CHEN Zhang-ping YANG MiR-140 downregulates fatty acid synthesis by targeting transforming growth factor alpha (TGFA) in bovine mammary epithelial cells Journal of Integrative Agriculture bovine mammary epithelial cells TGFA transcriptome sequencing miR-140 fatty acid metabolism |
| title | MiR-140 downregulates fatty acid synthesis by targeting transforming growth factor alpha (TGFA) in bovine mammary epithelial cells |
| title_full | MiR-140 downregulates fatty acid synthesis by targeting transforming growth factor alpha (TGFA) in bovine mammary epithelial cells |
| title_fullStr | MiR-140 downregulates fatty acid synthesis by targeting transforming growth factor alpha (TGFA) in bovine mammary epithelial cells |
| title_full_unstemmed | MiR-140 downregulates fatty acid synthesis by targeting transforming growth factor alpha (TGFA) in bovine mammary epithelial cells |
| title_short | MiR-140 downregulates fatty acid synthesis by targeting transforming growth factor alpha (TGFA) in bovine mammary epithelial cells |
| title_sort | mir 140 downregulates fatty acid synthesis by targeting transforming growth factor alpha tgfa in bovine mammary epithelial cells |
| topic | bovine mammary epithelial cells TGFA transcriptome sequencing miR-140 fatty acid metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S209531192200048X |
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