Partitioning of fluoxetine into mixed lipid bilayer containing 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)
In this study, the partitioning of fluoxetine, an antidepressant of selective serotonin reuptake inhibitor class into a mixture containing anionic and zwitterionic lipid vesicles was evaluated using second derivative spectrophotometry. The partition coefficients (Kp) of fluoxetine into the large un...
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| Format: | Article |
| Language: | English |
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Vietnam Ministry of Science and Technology
2022-03-01
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| Series: | Vietnam Journal of Science, Technology and Engineering |
| Subjects: | |
| Online Access: | https://vietnamscience.vjst.vn/index.php/vjste/article/view/165 |
| _version_ | 1797937756709584896 |
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| author | Anh T. Sy Vy T. Pham Trang T. Nguyen |
| author_facet | Anh T. Sy Vy T. Pham Trang T. Nguyen |
| author_sort | Anh T. Sy |
| collection | DOAJ |
| description |
In this study, the partitioning of fluoxetine, an antidepressant of selective serotonin reuptake inhibitor class into a mixture containing anionic and zwitterionic lipid vesicles was evaluated using second derivative spectrophotometry. The partition coefficients (Kp) of fluoxetine into the large unilamellar vesicles (LUVs) composed of zwitterionic 1,2-distearoyl-sn-glycero-3- phosphocholine (DSPC) containing 0 mol%, 10 mol%, 20 mol%, and 30 mol% of anionic 1,2-dipalmitoyl-snglycero-3-phosphoglycerol (DPPG) were measured in HEPES buffer at pH 7.4. The result revealed that when more negatively charged lipids incorporated into the LUVs, the condensing effect on the binary phospholipid membrane impeded the partitioning of positively charged fluoxetine, resulting in the decrease in the Kp values. This study adds a deeper understanding of how antidepressant fluoxetine exerts its effect on anioniccontaining biological membranes, shedding light onto drug delivery systems in the pharmaceutical field.
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| first_indexed | 2024-04-10T18:50:23Z |
| format | Article |
| id | doaj.art-6b272e254aac44ea8b81a4c5df75fc96 |
| institution | Directory Open Access Journal |
| issn | 2525-2461 2615-9937 |
| language | English |
| last_indexed | 2024-04-10T18:50:23Z |
| publishDate | 2022-03-01 |
| publisher | Vietnam Ministry of Science and Technology |
| record_format | Article |
| series | Vietnam Journal of Science, Technology and Engineering |
| spelling | doaj.art-6b272e254aac44ea8b81a4c5df75fc962023-02-01T08:13:04ZengVietnam Ministry of Science and TechnologyVietnam Journal of Science, Technology and Engineering2525-24612615-99372022-03-0161310.31276/VJSTE.61(3).16-24Partitioning of fluoxetine into mixed lipid bilayer containing 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)Anh T. Sy0Vy T. Pham1Trang T. Nguyen2School of Biotechnology, International University, Vietnam National University, Ho Chi Minh citySchool of Biotechnology, International University, Vietnam National University, Ho Chi Minh citySchool of Biotechnology, International University, Vietnam National University, Ho Chi Minh city In this study, the partitioning of fluoxetine, an antidepressant of selective serotonin reuptake inhibitor class into a mixture containing anionic and zwitterionic lipid vesicles was evaluated using second derivative spectrophotometry. The partition coefficients (Kp) of fluoxetine into the large unilamellar vesicles (LUVs) composed of zwitterionic 1,2-distearoyl-sn-glycero-3- phosphocholine (DSPC) containing 0 mol%, 10 mol%, 20 mol%, and 30 mol% of anionic 1,2-dipalmitoyl-snglycero-3-phosphoglycerol (DPPG) were measured in HEPES buffer at pH 7.4. The result revealed that when more negatively charged lipids incorporated into the LUVs, the condensing effect on the binary phospholipid membrane impeded the partitioning of positively charged fluoxetine, resulting in the decrease in the Kp values. This study adds a deeper understanding of how antidepressant fluoxetine exerts its effect on anioniccontaining biological membranes, shedding light onto drug delivery systems in the pharmaceutical field. https://vietnamscience.vjst.vn/index.php/vjste/article/view/165binary phospholipid membraneelectrostatic interactionfluoxetinepartition coefficientsecond derivative spectrophotometry |
| spellingShingle | Anh T. Sy Vy T. Pham Trang T. Nguyen Partitioning of fluoxetine into mixed lipid bilayer containing 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) Vietnam Journal of Science, Technology and Engineering binary phospholipid membrane electrostatic interaction fluoxetine partition coefficient second derivative spectrophotometry |
| title | Partitioning of fluoxetine into mixed lipid bilayer containing 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) |
| title_full | Partitioning of fluoxetine into mixed lipid bilayer containing 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) |
| title_fullStr | Partitioning of fluoxetine into mixed lipid bilayer containing 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) |
| title_full_unstemmed | Partitioning of fluoxetine into mixed lipid bilayer containing 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) |
| title_short | Partitioning of fluoxetine into mixed lipid bilayer containing 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) |
| title_sort | partitioning of fluoxetine into mixed lipid bilayer containing 1 2 dipalmitoyl sn glycero 3 phosphoglycerol dppg and 1 2 distearoyl sn glycero 3 phosphocholine dspc |
| topic | binary phospholipid membrane electrostatic interaction fluoxetine partition coefficient second derivative spectrophotometry |
| url | https://vietnamscience.vjst.vn/index.php/vjste/article/view/165 |
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