Collection of samples from women at different stages of pregnancy to search for early biomarkers of preterm birth
Aim. To create a collection of samples from women at different stages of pregnancy to search for early biomarkers of preterm birth.Material and methods. In order to standardize the sample collection, standard operation procedures have been developed with a step-by-step protocol for each research mem...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
«SILICEA-POLIGRAF» LLC
2020-12-01
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| Series: | Кардиоваскулярная терапия и профилактика |
| Subjects: | |
| Online Access: | https://cardiovascular.elpub.ru/jour/article/view/2708 |
| _version_ | 1797882395721990144 |
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| author | R. A. Illarionov O. V. Kosyakova E. S. Vashukova N. O. Yurkina M. O. Bakleicheva Yu. s. Dolgova T. A. Sushko D. R. Eremeeva M. S. Zainulina M. I. Yarmolinskaya O. N. Bespalova A. S. Glotov |
| author_facet | R. A. Illarionov O. V. Kosyakova E. S. Vashukova N. O. Yurkina M. O. Bakleicheva Yu. s. Dolgova T. A. Sushko D. R. Eremeeva M. S. Zainulina M. I. Yarmolinskaya O. N. Bespalova A. S. Glotov |
| author_sort | R. A. Illarionov |
| collection | DOAJ |
| description | Aim. To create a collection of samples from women at different stages of pregnancy to search for early biomarkers of preterm birth.Material and methods. In order to standardize the sample collection, standard operation procedures have been developed with a step-by-step protocol for each research member at the clinical (collection of medical data and biological material) and laboratory (transportation, sample preparation, storage, quality control) stages.Results. As of October 1, 2020, the collection includes peripheral blood samples from 182 women. Whole blood, serum, plasma, buffy coat and urine were collected during pregnancy, and placenta and umbilical cord blood samples — during labor. Clinical and medical history data was obtained about each pregnant woman, which includes data on the woman’s health status, the course and outcome of pregnancy. An electronic catalog has been created with information on samples (data on clinical characteristics and the number of aliquots of each sample type). The quality control (assessment of DNA and microRNA) was carried out, which showed the compliance of the obtained samples with the quality criteria and the preservation of initial characteristics during long-term storage. On the basis of collection, a study has begun to assess the level of microRNA expression in various types of biomaterial, in order to search for early biomarkers of premature birth.Conclusion. The creation of a collection of samples from pregnant women is a significant groundwork for future fundamental and applied research in various fields of biomedicine. This collection may provide an in-depth study of the pathogenesis of various pregnancy complications and the development of new methods for their diagnosis and treatment.- |
| first_indexed | 2024-04-10T03:34:03Z |
| format | Article |
| id | doaj.art-6b287675e23543f7a20d64994be01afc |
| institution | Directory Open Access Journal |
| issn | 1728-8800 2619-0125 |
| language | Russian |
| last_indexed | 2024-04-10T03:34:03Z |
| publishDate | 2020-12-01 |
| publisher | «SILICEA-POLIGRAF» LLC |
| record_format | Article |
| series | Кардиоваскулярная терапия и профилактика |
| spelling | doaj.art-6b287675e23543f7a20d64994be01afc2023-03-13T07:23:30Zrus«SILICEA-POLIGRAF» LLCКардиоваскулярная терапия и профилактика1728-88002619-01252020-12-0119610.15829/1728-8800-2020-27082229Collection of samples from women at different stages of pregnancy to search for early biomarkers of preterm birthR. A. Illarionov0O. V. Kosyakova1E. S. Vashukova2N. O. Yurkina3M. O. Bakleicheva4Yu. s. Dolgova5T. A. Sushko6D. R. Eremeeva7M. S. Zainulina8M. I. Yarmolinskaya9O. N. Bespalova10A. S. Glotov11ФГБНУ “НИИ акушерства, гинекологии и репродуктологии им. Д. О. Отта; Санкт-Петербургский государственный технологический институт (технический университет); Санкт-Петербургский государственный университетФГБНУ “НИИ акушерства, гинекологии и репродуктологии им. Д. О. ОттаФГБНУ “НИИ акушерства, гинекологии и репродуктологии им. Д. О. ОттаФГБНУ “НИИ акушерства, гинекологии и репродуктологии им. Д. О. ОттаФГБНУ “НИИ акушерства, гинекологии и репродуктологии им. Д. О. ОттаСПБ ГБУЗ «Родильный дом №6 им. В.Ф. Снегирева»СПБ ГБУЗ «Родильный дом №6 им. В.Ф. Снегирева»СПБ ГБУЗ «Родильный дом №6 им. В.Ф. Снегирева»СПБ ГБУЗ «Родильный дом №6 им. В.Ф. Снегирева»ФГБНУ “НИИ акушерства, гинекологии и репродуктологии им. Д. О. ОттаФГБНУ “НИИ акушерства, гинекологии и репродуктологии им. Д. О. ОттаФГБНУ “НИИ акушерства, гинекологии и репродуктологии им. Д. О. Отта; Санкт-Петербургский Государственный университетAim. To create a collection of samples from women at different stages of pregnancy to search for early biomarkers of preterm birth.Material and methods. In order to standardize the sample collection, standard operation procedures have been developed with a step-by-step protocol for each research member at the clinical (collection of medical data and biological material) and laboratory (transportation, sample preparation, storage, quality control) stages.Results. As of October 1, 2020, the collection includes peripheral blood samples from 182 women. Whole blood, serum, plasma, buffy coat and urine were collected during pregnancy, and placenta and umbilical cord blood samples — during labor. Clinical and medical history data was obtained about each pregnant woman, which includes data on the woman’s health status, the course and outcome of pregnancy. An electronic catalog has been created with information on samples (data on clinical characteristics and the number of aliquots of each sample type). The quality control (assessment of DNA and microRNA) was carried out, which showed the compliance of the obtained samples with the quality criteria and the preservation of initial characteristics during long-term storage. On the basis of collection, a study has begun to assess the level of microRNA expression in various types of biomaterial, in order to search for early biomarkers of premature birth.Conclusion. The creation of a collection of samples from pregnant women is a significant groundwork for future fundamental and applied research in various fields of biomedicine. This collection may provide an in-depth study of the pathogenesis of various pregnancy complications and the development of new methods for their diagnosis and treatment.-https://cardiovascular.elpub.ru/jour/article/view/2708беременностьбиобанкбиоресурсная коллекцияосложнения гестациипреждевременные роды |
| spellingShingle | R. A. Illarionov O. V. Kosyakova E. S. Vashukova N. O. Yurkina M. O. Bakleicheva Yu. s. Dolgova T. A. Sushko D. R. Eremeeva M. S. Zainulina M. I. Yarmolinskaya O. N. Bespalova A. S. Glotov Collection of samples from women at different stages of pregnancy to search for early biomarkers of preterm birth Кардиоваскулярная терапия и профилактика беременность биобанк биоресурсная коллекция осложнения гестации преждевременные роды |
| title | Collection of samples from women at different stages of pregnancy to search for early biomarkers of preterm birth |
| title_full | Collection of samples from women at different stages of pregnancy to search for early biomarkers of preterm birth |
| title_fullStr | Collection of samples from women at different stages of pregnancy to search for early biomarkers of preterm birth |
| title_full_unstemmed | Collection of samples from women at different stages of pregnancy to search for early biomarkers of preterm birth |
| title_short | Collection of samples from women at different stages of pregnancy to search for early biomarkers of preterm birth |
| title_sort | collection of samples from women at different stages of pregnancy to search for early biomarkers of preterm birth |
| topic | беременность биобанк биоресурсная коллекция осложнения гестации преждевременные роды |
| url | https://cardiovascular.elpub.ru/jour/article/view/2708 |
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