A Restriction Enzyme from Escherichia coli Purification and General Properties
An endonuclease restriction enzyme has been purified from E. coli about 40-fold withDNAse and RNAse recoveries of about 3%. The purification steps included precipitationof the enzyme with ammonium sulphate, and reclaimed it through Sephadex G-100 andDEAE-cellulose chromatography. The purified endonu...
Main Authors: | Mukaram Shikara, Nadia Tariq Barakat, Maysem Modaffer Al-Obaidy |
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Format: | Article |
Language: | English |
Published: |
Unviversity of Technology- Iraq
2009-03-01
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Series: | Engineering and Technology Journal |
Subjects: | |
Online Access: | https://etj.uotechnology.edu.iq/article_29309_4b1b6f4648f4387c4cdf6146dfff1428.pdf |
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