Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells.

Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells.

The low efficiency of differentiation into male germ cell (GC)-like cells and haploid germ cells from human embryonic stem cells (hESCs) reflects the culture method employed in the two-dimensional (2D)-microenvironment. In this study, we applied a three-step media and calcium alginate-based 3D-cultu...

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Bibliographic Details
Main Authors: Jung Jin Lim, Myung Sun Shim, Jeoung Eun Lee, Dong Ryul Lee
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090454&type=printable

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