Development of an Ex Vivo Assay for Identification of Infectious Hepatitis E Virus in Different Kinds of Food Samples
Hepatitis E virus (HEV) is a positive-sense single-stranded RNA virus and a major cause of acute viral hepatitis. HEV is responsible for 20 million infections worldwide in humans every year. HEV-3 and HEV-4 are zoonotic and are responsible for most of the HEV cases in developed countries. Consumptio...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/10/1231 |
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| author | Renate W. Hakze-van der Honing Sophie van Oort René A. M. Dirks Wim H. M. van der Poel |
| author_facet | Renate W. Hakze-van der Honing Sophie van Oort René A. M. Dirks Wim H. M. van der Poel |
| author_sort | Renate W. Hakze-van der Honing |
| collection | DOAJ |
| description | Hepatitis E virus (HEV) is a positive-sense single-stranded RNA virus and a major cause of acute viral hepatitis. HEV is responsible for 20 million infections worldwide in humans every year. HEV-3 and HEV-4 are zoonotic and are responsible for most of the HEV cases in developed countries. Consumption of contaminated pig meat or pig products is considered to be the main transmission route of HEV HEV-3 in Europe. Prevalence studies for HEV generally use PCR methods to detect the presence or absence of genomic RNA. However, these methods do not discriminate infectious virus particles from non-infectious material. Previously developed HEV cell culture systems only worked with high efficiency after cell line adaptation of the subjected virus strains. In this manuscript, the development of a culture system for the detection of infectious HEV strains is described. For this purpose, we optimized the isolation and the growth of primary hepatocytes from young piglets. Subsequently, the isolated hepatocytes were used to culture HEV of different origins, such as liver tissue samples and sausage samples. This method can be applied to better assess the risk of infection through consumption of food products associated with HEV RNA contamination. |
| first_indexed | 2024-03-10T20:59:37Z |
| format | Article |
| id | doaj.art-6b2b91fd044d4835b7606a1fe9a28a76 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T20:59:37Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-6b2b91fd044d4835b7606a1fe9a28a762023-11-19T17:40:21ZengMDPI AGPathogens2076-08172023-10-011210123110.3390/pathogens12101231Development of an Ex Vivo Assay for Identification of Infectious Hepatitis E Virus in Different Kinds of Food SamplesRenate W. Hakze-van der Honing0Sophie van Oort1René A. M. Dirks2Wim H. M. van der Poel3Wageningen Bioveterinary Research (WBVR), Wageningen University and Research, Houtribweg 39, 8221 Lelystad, The NetherlandsWageningen Bioveterinary Research (WBVR), Wageningen University and Research, Houtribweg 39, 8221 Lelystad, The NetherlandsWageningen Food Safety Research (WFSR), Wageningen University and Research, Akkermaalsbos 2, 6708 Wageningen, The NetherlandsWageningen Bioveterinary Research (WBVR), Wageningen University and Research, Houtribweg 39, 8221 Lelystad, The NetherlandsHepatitis E virus (HEV) is a positive-sense single-stranded RNA virus and a major cause of acute viral hepatitis. HEV is responsible for 20 million infections worldwide in humans every year. HEV-3 and HEV-4 are zoonotic and are responsible for most of the HEV cases in developed countries. Consumption of contaminated pig meat or pig products is considered to be the main transmission route of HEV HEV-3 in Europe. Prevalence studies for HEV generally use PCR methods to detect the presence or absence of genomic RNA. However, these methods do not discriminate infectious virus particles from non-infectious material. Previously developed HEV cell culture systems only worked with high efficiency after cell line adaptation of the subjected virus strains. In this manuscript, the development of a culture system for the detection of infectious HEV strains is described. For this purpose, we optimized the isolation and the growth of primary hepatocytes from young piglets. Subsequently, the isolated hepatocytes were used to culture HEV of different origins, such as liver tissue samples and sausage samples. This method can be applied to better assess the risk of infection through consumption of food products associated with HEV RNA contamination.https://www.mdpi.com/2076-0817/12/10/1231Hepatitis E virusHEVcell cultureinfectivityprimary hepatocytes |
| spellingShingle | Renate W. Hakze-van der Honing Sophie van Oort René A. M. Dirks Wim H. M. van der Poel Development of an Ex Vivo Assay for Identification of Infectious Hepatitis E Virus in Different Kinds of Food Samples Pathogens Hepatitis E virus HEV cell culture infectivity primary hepatocytes |
| title | Development of an Ex Vivo Assay for Identification of Infectious Hepatitis E Virus in Different Kinds of Food Samples |
| title_full | Development of an Ex Vivo Assay for Identification of Infectious Hepatitis E Virus in Different Kinds of Food Samples |
| title_fullStr | Development of an Ex Vivo Assay for Identification of Infectious Hepatitis E Virus in Different Kinds of Food Samples |
| title_full_unstemmed | Development of an Ex Vivo Assay for Identification of Infectious Hepatitis E Virus in Different Kinds of Food Samples |
| title_short | Development of an Ex Vivo Assay for Identification of Infectious Hepatitis E Virus in Different Kinds of Food Samples |
| title_sort | development of an ex vivo assay for identification of infectious hepatitis e virus in different kinds of food samples |
| topic | Hepatitis E virus HEV cell culture infectivity primary hepatocytes |
| url | https://www.mdpi.com/2076-0817/12/10/1231 |
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